Abstract
Ex situ conservation of tropical horticultural species is difficult using traditional conservation approaches as most of these produce nonorthodox seeds and many of these are vegetatively propagated. Clonally/vegetatively propagated tropical woody species are rather recalcitrant to in vitro establishment necessitating the need for alternate conservation methods. Cryopreservation of such difficult-to-store species has been attempted using diverse explants with invariably good success. Encouraging results while attempting cryopreservation of these species have further promoted application of vitrification-based cryotechniques to diverse plant germplasm during last two to three decades. Development of suitable cryopreservation protocols and long-term cryobanking using cryoprotocols are the two fundamental aspects of successful plant genetic resources conservation. Strengthening of basic research on cryopreservation of plant tissues, defined policy for prioritization of cryobanking of germplasm and need of quality human resource in this area are the issues which need further attention. Present chapter highlights the present status of cryopreservation of tropical horticulture species using diverse techniques and challenges in long-term cryobanking.
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Malik, S.K., Chaudhury, R. (2019). Cryopreservation Techniques for Conservation of Tropical Horticultural Species Using Various Explants. In: Rajasekharan, P., Rao, V. (eds) Conservation and Utilization of Horticultural Genetic Resources. Springer, Singapore. https://doi.org/10.1007/978-981-13-3669-0_19
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