Abstract
Over the past 30 years, stem cell technologies matured from an attractive option to investigate neurodegenerative diseases to a possible paradigm shift in their treatment through the development of cell-based regenerative medicine (CRM). Implantable cell replacement therapies promise to completely restore function of neural structures possibly changing how we currently perceive the onset of these conditions. One of the major clinical hurdles facing the routine implementation of stem cell therapy is the limited and inconsistent benefit observed thus far. While unclear, numerous pre-clinical and a handful of clinical cell fate imaging studies point to poor cell retention and survival. Coupling the need to better understand these mechanisms while providing scalable approaches to monitor these treatments in both pre-clinical and clinical scenarios, we show a proof of concept bioimpedance electronic platform for the Agile development of smart and mobile biomedical applications like neural implants or highly portable monitoring devices.
A. B. Cunha and C. Schuelke—Both authors contributed equally to this work.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
McAdams, E.T., Jossinet, J.: Tissue impedance: a historical overview. Physiol. Meas. 16(3A), A1. https://doi.org/10.1088/0967-3334/16/3a/001
Grimnes, S., Martinsen, Ø.G.: Bioimpedance and Bioelectricity Basics, 3rd edn. Academic Press is an Imprint of Elsevier, London (2015)
Ronaghi, M., Erceg, S., Moreno-Manzano, V., et al.: Challenges of stem cell therapy for spinal cord injury: human embryonic stem cells, endogenous neural stem cells, or induced pluripotent stem cells? Stem Cells 28(1), 93–99 (2010). https://doi.org/10.1002/stem.253
Nguyen, P.K., Neofytou, E., Rhee, J.-W., et al.: Potential strategies to address the major clinical barriers facing stem cell regenerative therapy for cardiovascular disease: a review. JAMA Cardiol. 1(8), 953–962 (2016). https://doi.org/10.1001/jamacardio.2016.2750
Xiao, C., Luong, J.H.T.: On-line monitoring of cell growth and cytotoxicity using electric cell-substrate impedance sensing (ECIS). Biotechnol. Prog. 19(3), 1000–1005 (2003). https://doi.org/10.1021/bp025733x
Krinke, D., Jahnke, H.-G., Mack, T.G.A., et al.: A novel organotypic tauopathy model on a new microcavity chip for bioelectronic label-free and real time monitoring. Biosens. Bioelectron. 26(1), 162–168 (2010). https://doi.org/10.1016/j.bios.2010.06.002
Hug, T.S.: Biophysical methods for monitoring cell-substrate interactions in drug discovery. Assay Drug Dev. Technol. 1(3), 479–488 (2003). https://doi.org/10.1089/154065803322163795
Jahnke, H.-G., Braesigk, A., Mack, T.G.A., et al.: Impedance spectroscopy based measurement system for quantitative and label-free real-time monitoring of tauopathy in hippocampal slice cultures. Biosens. Bioelectron. 32(1), 250–258 (2012). https://doi.org/10.1016/j.bios.2011.12.026
Haas, S., Jahnke, H.-G., Glass, M., et al.: Real-time monitoring of relaxation and contractility of smooth muscle cells on a novel biohybrid chip. Lab Chip 10(21), 2965–2971 (2010). https://doi.org/10.1039/c0lc00008f
Seidel, D., Obendorf, J., Englich, B., et al.: Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays. Biosens. Bioelectron. 86, 277–286 (2016). https://doi.org/10.1016/j.bios.2016.06.056
Kumar, S.: Reducing complexity and cost for portable medical electronics through system on chip architectures (2010). https://www.cypress.com/file/102926/download. Accessed 09 Jan 2020
Saunders, M.: Software development models for PSoC 6 (2017). https://www.cypress.com/blog/technical/software-development-models-psoc-6. Accessed 09 Jan 2020
CORDIS: European Training Network for Cell-based Regenerative Medicine | Projects | H2020 | CORDIS | European Commission (2019). https://cordis.europa.eu/project/rcn/205439/factsheet/en. Accessed 28 Feb 2019
Villa, A., Liste, I., Courtois, E.T., et al.: Generation and properties of a new human ventral mesencephalic neural stem cell line. Exp. Cell Res. 315(11), 1860–1874 (2009). https://doi.org/10.1016/j.yexcr.2009.03.011
Villa, A., Snyder, E.Y., Vescovi, A., et al.: Establishment and properties of a growth factor-dependent, perpetual neural stem cell line from the human CNS. Exp. Neurol. 161(1), 67–84 (2000). https://doi.org/10.1006/exnr.1999.7237
Tønnesen, J., Seiz, E.G., Ramos, M., et al.: Functional properties of the human ventral mesencephalic neural stem cell line hVM1. Exp. Neurol. 223(2), 653–656 (2010). https://doi.org/10.1016/j.expneurol.2010.01.013
Krabbe, C., Courtois, E., Jensen, P., et al.: Enhanced dopaminergic differentiation of human neural stem cells by synergistic effect of Bcl-xL and reduced oxygen tension. J. Neurochem. 110(6), 1908–1920 (2009). https://doi.org/10.1111/j.1471-4159.2009.06281.x
Courtois, E.T., Castillo, C.G., Seiz, E.G., et al.: In vitro and in vivo enhanced generation of human A9 dopamine neurons from neural stem cells by Bcl-XL. J. Biol. Chem. 285(13), 9881–9897 (2010). https://doi.org/10.1074/jbc.M109.054312
Hassan, Y.M., Caviglia, C., Hemanth, S., et al.: High temperature SU-8 pyrolysis for fabrication of carbon electrodes. J. Anal. Appl. Pyrolysis 125, 91–99 (2017). https://doi.org/10.1016/j.jaap.2017.04.015
Witzel, F., Fritsche-Guenther, R., Lehmann, N., et al.: Analysis of impedance-based cellular growth assays. Bioinformatics 31(16), 2705–2712 (2015). https://doi.org/10.1093/bioinformatics/btv216
Acknowledgment
This project has been funded by the European Union Horizon 2020 Programme (H2020-MSCA-ITN-2016) under the Marie Skłodowska-Curie Innovative Training Network and Grant Agreement No.722779.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Ethics declarations
The authors declare that they have no conflict of interest.
Rights and permissions
Copyright information
© 2020 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Cunha, A.B. et al. (2020). Bioimpedance Measurements on Human Neural Stem Cells as a Benchmark for the Development of Smart Mobile Biomedical Applications. In: Bertemes-Filho, P. (eds) 17th International Conference on Electrical Bioimpedance. ICEBI 2019. IFMBE Proceedings, vol 72. Springer, Singapore. https://doi.org/10.1007/978-981-13-3498-6_6
Download citation
DOI: https://doi.org/10.1007/978-981-13-3498-6_6
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-3497-9
Online ISBN: 978-981-13-3498-6
eBook Packages: EngineeringEngineering (R0)