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Immobilized β-Galactosidase-Mediated Conversion of Lactose: Process, Kinetics and Modeling Studies pp 1–8Cite as

Introduction

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Abstract

This chapter provides a brief overview of the research background in relation to β-galactosidase-catalyzed conversion of lactose. The essential for lactose conversion is described in this chapter. Lactose can be converted to various types of product, and this research is utilizing β-galactosidase as a biocatalyst which results in the formation of saccharides. Therefore, β-galactosidase-catalyzed reaction with lactose as a substrate is introduced in the following subsection. This section also introduces the problem statement, research objectives, and the scope of study.

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References

  1. Albayrak N, Yang ST (2002) Production of galacto-oligosaccharides from lactose by Aspergillus oryzae β-galactosidase immobilized on cotton cloth. Biotechnol Bioeng 77(1):8–19

    Article  CAS  Google Scholar 

  2. Aronson M (1952) Transgalactosidation during lactose hydrolysis. Arch Biochem Biophys 39(2):370–378

    Article  CAS  Google Scholar 

  3. Carvalho F, Prazeres AR, Rivas J (2013) Cheese whey wastewater: characterization and treatment. Sci Total Environ 445:385–396

    Article  Google Scholar 

  4. Chan C, Bérubé P, Hall E (2011) Relationship between types of surface shear stress profiles and membrane fouling. Water Res 45(19):6403–6416

    Article  CAS  Google Scholar 

  5. Chockchaisawasdee S, Athanasopoulos VI, Niranjan K, Rastall RA (2005) Synthesis of galacto-oligosaccharide from lactose using β-galactosidase from Kluyveromyces lactis: Studies on batch and continuous UF membrane-fitted bioreactors. Biotechnol Bioeng 89(4):434–443

    Article  CAS  Google Scholar 

  6. Cohen Y (1988) Hydrodynamic thickness of adsorbed polymers in steady shear flow. Macromolecules 21(2):494–499

    Article  CAS  Google Scholar 

  7. Crittenden R, Playne MJ (1996) Production, properties and applications of food-grade oligosaccharides. Trends Food Sci Technol 7(11):353–361

    Article  CAS  Google Scholar 

  8. Cuartas-Uribe B, Alcaina-Miranda M, Soriano-Costa E, Mendoza-Roca J, Iborra-Clar M, Lora-García J (2009) A study of the separation of lactose from whey ultrafiltration permeate using nanofiltration. Desalination 241(1):244–255

    Article  CAS  Google Scholar 

  9. Foda MI, Lopez-Leiva M (2000) Continuous production of oligosaccharides from whey using a membrane reactor. Process Biochem 35(6):581–587

    Article  CAS  Google Scholar 

  10. Gropper SS, Smith JL (2012) Advanced nutrition and human metabolism, 6th edn. Wadsworth Publishing, California

    Google Scholar 

  11. Güleç H, Gürdaş S, Albayrak N, Mutlu M (2010) Immobilization of Aspergillus oryzae β-galactosidase on low-pressure plasma-modified cellulose acetate membrane using polyethyleneimine for production of galactooligosaccharide. Biotechnol Bioproc E 15(6):1006–1015

    Article  Google Scholar 

  12. Güleç HA (2013) Immobilization of β-galactosidase from Kluyveromyces lactis onto polymeric membrane surfaces: effect of surface characteristics. Colloids Surf B 104:83–90

    Article  Google Scholar 

  13. Heyman MB (2006) Lactose intolerance in infants, children, and adolescents. Pediatrics 118(3):1279–1286

    Article  Google Scholar 

  14. Hueso P, Martin-Sosa S, Martin M-J (2005) Role of milk carbohydrates in preventing bacterial adhesion. In: Yarema KJ (ed) Handbook of carbohydrate engineering. Taylor & Francis Group, London, p 142

    Google Scholar 

  15. Iwasaki K-I, Nakajima M, Nakao S-I (1996) Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase. Process Biochem 31(1):69–76

    Article  CAS  Google Scholar 

  16. Jovanovic-Malinovska R, Fernandes P, Winkelhausen E, Fonseca L (2012) Galacto-oligosaccharides synthesis from lactose and whey by β-galactosidase immobilized in PVA. Appl Biochem Biotechnol 168(5):1197–1211

    Article  CAS  Google Scholar 

  17. Juers DH, Matthews BW, Huber RE (2012) LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci 21(12):1792–1807

    Article  CAS  Google Scholar 

  18. Keim NL, Levin RJ, Havel PJ (2014) Carbohydrates. In: Ros AC, Caballero B, Cousins RJ, Tucker KL, Ziegler TR (eds) Modern nutrition in health and disease, 11st ed. Lippincott Williams & Wilkins, Philadelphia, p 74

    Google Scholar 

  19. Kushwaha JP, Srivastava VC, Mall ID (2010) Treatment of dairy wastewater by inorganic coagulants: parametric and disposal studies. Water Res 44(20):5867–5874

    Article  CAS  Google Scholar 

  20. Mahoney RR (1998) Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chem 63(2):147–154

    Article  CAS  Google Scholar 

  21. Mateo C, Monti R, Pessela BC, Fuentes M, Torres R, Manuel Guisán J, Fernández‐Lafuente R (2004) Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose. Full hydrolysis of lactose in milk. Biotechnol Prog 20 (4):1259–1262

    Google Scholar 

  22. Mozaffar Z, Nakanishi K, Matsuno R (1986) Continuous production of galacto-oligosaccharides from lactose using immobilized β-galactosidase from Bacillus circulans. Appl Microbiol Biotechnol 25(3):224–228

    CAS  Google Scholar 

  23. Nagy E (2012) Basic equations of the mass transport through a membrane layer. Elsevier, London

    Google Scholar 

  24. Nath A, Bhattacharjee C, Chowdhury R (2013) Synthesis and separation of galacto-oligosaccharides using membrane bioreactor. Desalination 316:31–41

    Article  CAS  Google Scholar 

  25. Neri DF, Balcão VM, Costa RS, Rocha IC, Ferreira EM, Torres DP, Rodrigues LR, Carvalho LB, Teixeira JA (2009) Galacto-oligosaccharides production during lactose hydrolysis by free Aspergillus oryzae β-galactosidase and immobilized on magnetic polysiloxane-polyvinyl alcohol. Food Chem 115(1):92–99

    Article  CAS  Google Scholar 

  26. Palai T, Bhattacharya PK (2013) Kinetics of lactose conversion to galacto-oligosaccharides by β-galactosidase immobilized on PVDF membrane. J Biosci Bioeng 115(6):668–673

    Article  CAS  Google Scholar 

  27. Panesar PS, Kennedy JF, Gandhi DN, Bunko K (2007) Bioutilisation of whey for lactic acid production. Food Chem 105(1):1–14

    Article  CAS  Google Scholar 

  28. Park A-R, Oh D-K (2010) Galacto-oligosaccharide production using microbial β-galactosidase: Current state and perspectives. Appl Microbiol Biotechnol 85(5):1279–1286

    Article  CAS  Google Scholar 

  29. Sadava DE, Heller HC, Orians GH, Purves WK, Hillis DM (2013) E-book for life: the science of biology, 10th edn. Palgrave Macmillan, London

    Google Scholar 

  30. Saltzman JR, Russell RM (1998) The aging gut: nutritional issues. Gastroenterol Clin North Am 27(2):309–324

    Article  CAS  Google Scholar 

  31. Shin H-J, Yang J-W (1994) Galacto-oligosaccharide production by β-galactosidase in hydrophobic organic media. Biotech Lett 16(11):1157–1162

    Article  CAS  Google Scholar 

  32. Starr C, Evers CA, Starr L (2011) Biology: concepts and applications without physiology. Cengage Learning, Boston

    Google Scholar 

  33. Szczodrak J (2000) Hydrolysis of lactose in whey permeate by immobilized β-galactosidase from Kluyveromyces fragilis. J Mol Catal B Enzym 10(6):631–637

    Article  CAS  Google Scholar 

  34. Ulbricht M, Riedel M, Marx U (1996) Novel photochemical surface functionalization of polysulfone ultrafiltration membranes for covalent immobilization of biomolecules. J Membr Sci 120(2):239–259

    Article  CAS  Google Scholar 

  35. Urrutia P, Rodriguez-Colinas Br, Fernandez-Arrojo L, Ballesteros AO, Wilson L, Illanes As, Plou FJ (2013) Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae. J Agric Food Chem 61(5):1081–1087

    Article  CAS  Google Scholar 

  36. Zheng P, Yu H, Sun Z, Ni Y, Zhang W, Fan Y, Xu Y (2006) Production of galacto-oligosaccharides by immobilized recombinant β-galactosidase from Aspergillus candidus. Biotechnol J 1(12):1464–1470

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Chemical Engineering, Universiti Sains Malaysia, Penang, Malaysia

    Fadzil Noor Gonawan

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  1. Fadzil Noor Gonawan
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Correspondence to Fadzil Noor Gonawan .

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Gonawan, F.N. (2019). Introduction. In: Immobilized β-Galactosidase-Mediated Conversion of Lactose: Process, Kinetics and Modeling Studies. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-3468-9_1

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