Genetic Engineering for the Improvement of Oil Content and Associated Traits in Jatropha curcas L.
Interminably increasing petroleum rates and exhaustion of fossil reserves have ignited a global search for substitutes to renewable fuel sources. Many oil-generating plants, crops and trees have been considered for biofuel; among these Jatropha curcas is regarded as one of the most promising oilseed plants as its seeds contain oil content up to 35%. Because fossil oil consumption is increasing day-by-day, there is an urgent need to enhance the oil content. Transgenic technology is one of the advanced techniques that have been applied to enhance oil content and modify the composition of fatty acids in seed oils. Increasing seed oil content can be done by modifying the enzyme’s level expression in the triacylglycerol biosynthetic pathway. In this chapter, an effort is made to highlight the potential of transgenic technology towards the enhancement of the oil content and in altering the candidate gene expression for biosynthesis of triacylglycerol.
KeywordsFatty acids Jatropha Kennedy pathway Renewable biodiesel Triacylglycerols
The manuscript number is PRIS (PRIS- CSIR-CSMCRI - 190/2018).
- Banapurmath NR, Hosmath RS, Girish NM et al (2012) Combustion of Jatropha curcas oil, methyl esters and blends with diesel or ethanol in a CI engine (Ch. 29). In: Carels N, Sujatha M, Bahadur B (eds) Jatropha, challenges for a new energy crop: volume 1: Farming, economics and biofuel. Springer, New York, pp 557–570. https://doi.org/10.1007/978-1-4614-4806-8_29 CrossRefGoogle Scholar
- Brittaine R, Lutaladio N (2010) Jatropha: a smallholder bioenergy crop: the potential for pro-poor development, Integrated Crop Management. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
- Lung SC, Weselake RJ (2006) Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids 41:1073–1088Google Scholar
- Nindita A, Iswari SD, Bambang SP et al (2015) Genetic improvement and biotechnology research of Jatropha curcas Linn. Review: future research opportunity and sustainability challenges in Indonesia. Conference and exhibition Indonesia – new, renewable energy and energy conservation (The 3rd Indo-EBTKE ConEx 2014)Google Scholar
- O’Hara P, Slabas AR, Fawcett T (2002) Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis. Plant Physiol 12:9310–9320Google Scholar
- Raorane M, Populechai S, Gatehouse AMR et al (2013) Proteomic perspectives on understanding and improving Jatropha curcas L. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop volume 2: Genetic improvement and biotechnology. Springer, New York, pp 375–391Google Scholar
- Somerville C, Browse J, Jaworski JG et al (2000) Lipids. In: Buchanan BB, Gruissem W, Jones RL (eds) Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville, pp 456–527Google Scholar
- Stymne SS (1987) Triacylglycerol biosynthesis. In: The biochemistry of plants: a comprehensive treatise. Academic, Orlando, pp 175–214Google Scholar
- Xu J, Francis T, Mietkiewska E et al (2008) Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content. Plant Biotechnol J 6:799–818CrossRefGoogle Scholar
- Ye J, Hong Y, Qu J et al (2013) Improvement of J. curcas oil by genetic transformation. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop volume 2: Genetic improvement and biotechnology. Springer, New York, pp 547–562Google Scholar