Proteomic Studies in Jatropha curcas Seeds
Jatropha curcas (L.) has gained interest when it is realized as a potential source of vegetable oil for biodiesel production, a process that results with a press-cake rich in protein. However, press-cake cannot be used without processing due to its high content of toxic and antinutritional compounds. The Jatropha genetic resources remain poorly characterized; however in Mexico exists in the wild a non-toxic J. curcas genotype with high potential as a source of information for plant breeding in order to obtain varieties with increased oil contents and low amount of toxic compounds that will increase the use of press-cake as feedstock. Then it is necessary to unravel the mechanisms of triacylglycerol and antinutrients biosynthesis pathways. Proteomics is a powerful tool that has been used to identify the proteins that are accumulated in the different seed tissues, e.g. endosperm, integument, oil bodies, and plastids, with the aim to generate information about key enzymes that could be potential targets for the development of new strategies for the selective breeding of Jatropha.
KeywordsEndosperm Integuments Non-toxic genotypes Oil bodies Seed storage proteins Shotgun analysis Two-dimensional gel electrophoresis
We thank Dr. Miguel A. Angulo Escalante-CIAD Culiacan Mexico for providing Jatropha seeds, Iván Takeshi Cerritos Castro for Jatropha pictures, and Ofelia Rico for manuscript reviewing.
- Costa GG, Cardoso KC, Del Bem LE et al (2010) Transcriptome analysis of the oil-rich seed of the bioenergy crop Jatropha curcas L. BMC Genomics 11:462Google Scholar
- Greenwood JS, Helm M, Gleti C (2005) Ricinosomes and endosperm transfer cell structure in programmed cell death of the nucellus during Ricinus seed development. Proc Natl Acad Sci U S A 103:2238–2243Google Scholar
- Liu P, Wang CM, Li L et al (2011a) Mapping QTLs for oil traits and eQTLs for oleosin genes in jatropha. BMC Plant Biol 11:132Google Scholar
- Liu H, Yang Z, Yang M et al (2011b) The differential proteome of endosperm and embryo from mature seed of Jatropha curcas. Plant Sci 181:660–666Google Scholar
- Mandal S, Mandal RK (2000) Seed storage proteins and approaches for improvement of their nutritional quality by genetic engineering. Curr Sci 79:576–589Google Scholar
- Martinez-Herrera J, Siddhuraju P, Francis G et al (2006) Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico. Food Chem 96:80–89Google Scholar
- Ovando-Medina I, Espinosa-García FJ, Núñez-Farfán JS et al (2011) State of the art of genetic diversity research in Jatropha curcas L. Sci Res Essays 6:1709–1719Google Scholar
- Raorane M, Popluechai S, Gatehouse AMR et al (2013) Proteomic perspectives on understanding and improving Jatropha curcas L. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop (Vol 2): Genetic improvement and biotechnology. Springer, New York, pp 375–391Google Scholar
- Rogowska-Wrzesinska A, Le Bihan MC, Thaysen-Andersen M et al (2013) 2D gels still have a niche in proteomics. J Proteome 88:4–13Google Scholar
- Rotundo JL, Westgate ME (2009) Meta-analysis of environmental effects on soybean seed composition. Field Crops Res 110:147–156Google Scholar