Culturing and Maintaining Mammalian Cell Culture

  • Raha Ahmad RausEmail author
  • Ghassan Ali Salih
  • Mustaffa Nameer Shaban


Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and intercellular activities and flux; genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery and development can be carried out without manipulation of living animals. In this chapter, detail protocol of media preparation, cell culture maintenance and preservation are elaborated for both types of mammalian cell culture, monolayer or suspension cultures. Determination of number of cells is discussed as well.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Raha Ahmad Raus
    • 1
    Email author
  • Ghassan Ali Salih
    • 1
  • Mustaffa Nameer Shaban
    • 1
  1. 1.Department of Biotechnology Engineering, Faculty of EngineeringInternational Islamic University MalaysiaKuala LumpurMalaysia

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