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In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM

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Abstract

Consisting of viscous phospholipid bilayer, different kinds of proteins and various nano/micrometer-sized domains, cell membranes have proven to play very important roles in ensuring the stability of the intracellular environment and order of cellular signal transductions. The developments of modern cell biology, immunology, and medicine urge us to explore more precise cell membrane structures and detailed functions of biomolecules on cell membranes. Due to the minuscule size of biomolecules and their clusters on cell membranes (varying from several nanometers to hundreds of nanometers), a high resolution microscopy is needed to explore the cell membrane biomolecule distribution.

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Acknowledgment

This work is supported by China Postdoctoral Science Foundation (2018M631026) and Macau Science and Technology Development Fund (Grant No. 028/2014/A1). This chapter was adapted from the paper published by our group in Nanoscale (Pi Jiang, Jin Hua, Yang Fen, Chen ZhengW., Cai Jiye, 2014 Nov 7; 6; 21; 12229-12249). The related contents are reproduced with permission from The Royal Society of Chemistry.

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Authors and Affiliations

  1. Department of Immunology and Microbiology, University of Illinois at Chicago, Chicago, IL, USA

    Jiang Pi

  2. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China

    Jiang Pi, Hua Jin & Jiye Cai

  3. Feinberg School of Medicine, Northwestern University, Evanston, IL, USA

    Hua Jin

  4. Department of Chemistry, Jinan University, Jinan, China

    Jiye Cai

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  1. Jiang Pi
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  2. Hua Jin
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Correspondence to Jiye Cai .

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  1. State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China

    Jiye Cai

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Pi, J., Jin, H., Cai, J. (2018). In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM. In: Cai, J. (eds) Atomic Force Microscopy in Molecular and Cell Biology. Springer, Singapore. https://doi.org/10.1007/978-981-13-1510-7_3

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