Skip to main content
SpringerLink
Log in

Biomimetic Extracellular Matrices and Scaffolds Prepared from Cultured Cells

  • Chapter
  • First Online:
Cutting-Edge Enabling Technologies for Regenerative Medicine

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1078))

Abstract

Extracellular matrix (ECM) interacts with cells and provides important signals to control cell functions and to maintain homeostasis of living organisms. Composition of ECM in each tissue is dependent on cell type and cell phenotype. ECM also dynamically changes its composition during stem cell differentiation and tissue development. Various ECM substrates and scaffolds have been prepared for stem cells culture and tissue engineering. They can be reconstructed by using isolated ECM components or acellular matrices from different tissues and organs. In recent years, cultured cells have been used as a useful source to prepare biomimetic ECM substrates and scaffolds. ECM derived from different cell type can be prepared by culturing the respective cells to allow the cells to secrete desirable ECM components. Furthermore, dynamically changing ECM can be prepared by controlling the stepwise differentiation of stem cells. The composition of the biomimetic ECM substrates and scaffolds changes with cell type and has different effects on differentiation of stem cells. The latest progress on biomimetic ECM substrates and scaffolds derived from cultured cells is summarized and highlighted.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Giancotti FG, Ruoslahti E (1999) Integrin signaling. Science 285:1028–1032

    Article  CAS  Google Scholar 

  2. Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110:673–687

    Article  CAS  Google Scholar 

  3. Lutolf MP, Hubbell JA (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23:47–55

    Article  CAS  Google Scholar 

  4. Griffith LG, Naughton G (2002) Tissue engineering – current challenges and expanding opportunities. Science 295:1009–1014

    Article  CAS  Google Scholar 

  5. Chen CW, Tsai YH, Deng WP, Shih SN, Fang CL, Burch JG, Chen WH, Lai WF (2005) Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells. J Orthop Res 23:446–453

    Article  CAS  Google Scholar 

  6. Kino-Oka M, Yashiki S, Ota Y, Mushiaki Y, Sugawara K, Yamamoto T, Takezawa T, Taya M (2005) Subculture of chondrocytes on a collagen type I-coated substrate with suppressed cellular dedifferentiation. Tissue Eng 11:597–608

    Article  CAS  Google Scholar 

  7. Hoshiba T, Wakejima M, Cho CS, Shiota G, Akaike T (2008) Different regulation of hepatocyte behaviors between natural extracellular matrices and synthetic extracellular matrices by HGF. J Biomed Mater Res 85A:228–235

    Article  CAS  Google Scholar 

  8. Nagata N, Iwanaga A, Inoue K, Tabata Y (2002) Co-culture of extracellular matrix suppresses the cell death of rat pancreatic islets. J Biomater Sci Polym Ed 13:579–590

    Article  CAS  Google Scholar 

  9. Yi JY, Yoon YH, Park HS, Kim CH, Kim CH, Kang HJ, Lee E, Kim YY, Jin YJ, Kim TH, Son YS (2001) Reconstruction of basement membrane in skin equivalent; role of laminin-1. Arch Dermatol Res 293:356–362

    Article  CAS  Google Scholar 

  10. Hoshiba T, Lu H, Kawazoe N, Chen G (2010) Decellularized matrices for tissue engineering. Expert Opin Biol Ther 10:1717–1728

    Article  CAS  Google Scholar 

  11. Hedman K, Kurkinen M, Alitalo K, Vaheri A, Johansson S, Höök M (1979) Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol 81:83–91

    Article  CAS  Google Scholar 

  12. Chen LB, Murray A, Segal RA, Bushnell A, Walsh ML (1978) Studies on intercellular LETS glycoprotein matrices. Cell 14:377–391

    Article  CAS  Google Scholar 

  13. Pham MT, Reuther H, Maitz MF (2003) Native extracellular matrix coating on Ti surfaces. J Biomed Mater Res 66A:310–316

    Article  CAS  Google Scholar 

  14. Furuyama A, Kimata K, Mochitate K (1997) Assembly of basement membrane in vitro by cooperation between alveolar epithelial cells and pulmonary fibroblasts. Cell Struct Funct 22:603–614

    Article  CAS  Google Scholar 

  15. Furuyama A, Mochitate K (2000) Assembly of the exogenous extracellular matrix during basement membrane formation by alveolar epithelial cells in vitro. J Cell Sci 113:859–868

    CAS  PubMed  Google Scholar 

  16. Hoshiba T, Mochitate K, Akaike T (2007) Hepatocytes maintain their function on basement membrane formed by epithelial cells. Biochem Biophys Res Commun 359:151–156

    Article  Google Scholar 

  17. Hosokawa T, Betsuyaku T, Nishimura M, Furuyama A, Katagiri K, Mochitate K (2007) Differentiation of tracheal basal cells to ciliated cells and tissue reconstruction on the synthesized basement membrane substratum in vitro. Connect Tissue Res 48:9–18

    Article  CAS  Google Scholar 

  18. Hoshiba T, Lu H, Yamada T, Kawazoe N, Tateishi T, Chen G (2011) Effects of extracellular matrices derived from different cell sources on chondrocyte functions. Biotechnol Prog 27:788–795

    Article  CAS  Google Scholar 

  19. Hoshiba T, Tomoe Y, Lu H, Kawazoe N, Chen G (2012) Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocytes expansion. J Biomed Mater Res 100 A:694–702

    Article  Google Scholar 

  20. Cai R, Kawazoe N, Chen G (2015) Influence of surfaces modified with biomimetic extracellular matrices on adhesion and proliferation of mesenchymal stem cells and osteosarcoma cells. Colloids Surf B Biointerfaces 126:381–386

    Article  CAS  Google Scholar 

  21. D’Amour KA, Bang AG, Eliazer S, Kelly OG, Agulnick AD, Smart NG, Moorman MA, Kroon E, Carpenter MK, Bartge EE (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24:1392–1401

    Article  Google Scholar 

  22. Nishimura R, Hata K, Ikeda F, Ichida F, Shimoyama A, Matsubara T, Wada M, Amano K, Yoneda T (2008) Signal transduction and transcriptional regulation during mesenchymal cell differentiation. J Bone Miner Metab 26:203–212

    Article  Google Scholar 

  23. Daley WP, Peters SB, Larsen M (2008) Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 121:255–264

    Article  CAS  Google Scholar 

  24. Page-McCaw A, Ewald AJ, Werb Z (2007) Matrix metalloproteinases and the regulation of tissue remodelling. Nat Rev Mol Cell Biol 8:221–233

    Article  CAS  Google Scholar 

  25. Midwood KS, Williams LV, Schwarzbauer JE (2004) Tissue repair and the dynamics of the extracellular matrix. Int J Biochem Cell Biol 36:1031–1037

    Article  CAS  Google Scholar 

  26. Hoshiba T, Kawazoe N, Tateishi T, Chen G (2009) Development of stepwise osteogenesis-mimicking matrices for the regulation of mesenchymal stem cell functions. J Biol Chem 284:31164–31173

    Article  CAS  Google Scholar 

  27. Hoshiba T, Kawazoe N, Tateishi T, Chen G (2010) Development of extracellular matrices mimicking stepwise adipogenesis of mesenchymal stem cells. Adv Mater 22:3042–3047

    Article  CAS  Google Scholar 

  28. Hoshiba T, Kawazoe N, Chen G (2012) The balance of osteogenic and adipogenic differentiation in human mesenchymal stem cells by matrices that mimic stepwise tissue development. Biomaterials 33:2025–2031

    Article  CAS  Google Scholar 

  29. Cai R, Nakamoto T, Kawazoe N, Hoshiba T, Chen G (2016) Matrices secreted during simultaneous osteogenesis and adipogenesis of mesenchymal stem cells affect stem cells differentiation. Acta Biomater 35:185–193

    Article  CAS  Google Scholar 

  30. Lu H, Hoshiba T, Kawazoe N, Tateishi T, Chen G (2011) Autologous extracellular matrix scaffolds for tissue engineering. Biomaterials 32:2489–2499

    Article  CAS  Google Scholar 

  31. Lu H, Hoshiba T, Kawazoe N, Koda I, Song M, Chen G (2011) Cultured cell-derived extracellular matrix scaffolds for tissue engineering. Biomaterials 32:9658–9666

    Article  CAS  Google Scholar 

  32. Cai R, Nakamoto T, Kawazoe N, Chen G (2015) Influence of stepwise chondrogenesis-mimicking 3D extracellular matrix on chondrogenic differentiation of mesenchymal stem cells. Biomaterials 52:199–207

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by KAKENHI Grant Number 15H03027 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

Author information

Authors and Affiliations

  1. Research Center for Functional Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan

    Guoping Chen & Naoki Kawazoe

Authors
  1. Guoping Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoki Kawazoe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guoping Chen .

Editor information

Editors and Affiliations

  1. The Catholic University of Korea, Seoul, Korea (Republic of)

    Heung Jae Chun

  2. Hallym University, Gangwon-do, Korea (Republic of)

    Chan Hum Park

  3. Kyung Hee University, Seoul, Korea (Republic of)

    Il Keun Kwon

  4. Chonbuk National University, Jeonju, Korea (Republic of)

    Gilson Khang

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Chen, G., Kawazoe, N. (2018). Biomimetic Extracellular Matrices and Scaffolds Prepared from Cultured Cells. In: Chun, H., Park, C., Kwon, I., Khang, G. (eds) Cutting-Edge Enabling Technologies for Regenerative Medicine. Advances in Experimental Medicine and Biology, vol 1078. Springer, Singapore. https://doi.org/10.1007/978-981-13-0950-2_24

Download citation

Publish with us

Policies and ethics

Search

Navigation