Abstract
Michaelis–Menten formalism undoubtedly is a very important first approximation for any new enzyme to be studied. Although simple and elegant, it has only a limited range of applicability. Not all enzymes adhere to Michaelis–Menten kinetics, and there are notable exceptions. Appropriate kinetic experimental design allows us to make this judgment. Collating good quality kinetic data is the first task in enzyme characterization.
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Punekar, N.S. (2018). Enzyme Kinetic Data: Collection and Analysis. In: ENZYMES: Catalysis, Kinetics and Mechanisms. Springer, Singapore. https://doi.org/10.1007/978-981-13-0785-0_17
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DOI: https://doi.org/10.1007/978-981-13-0785-0_17
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