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Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications

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Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications

Part of the book series: Microorganisms for Sustainability ((MICRO,volume 8))

Abstract

Turkey has a great number of different ecological areas, owning over 200 hot water resources and various hypersaline environments with a broad microbial diversity and opportunities for newly isolated microorganisms from extreme environments for many industrial applications. A variety of thermophilic and halophilic microorganisms in different regions of Turkey have been isolated and identified. The thermophilic bacterial members studied were Anoxybacillus, Geobacillus, Bacillus, Brevibacillus, and Aeribacillus belonging to the Bacillaceae family and the other thermophiles such as Thermus and Thermomonas, whereas the isolated halophilic microorganisms were mainly found to be members of the archaeal family Halobacteriaceae or grouped into bacterial phylum Bacteroidetes. In summary, the present study reviews on (1) isolating and identifying thermophiles and halophiles single or as community from various extreme habitats in Turkey based on conventional (morphological, physiological and biochemical tests) and/or molecular methods, (2) screening these extremophiles for industrially important enzymes, (3) studying other novel products and their use in other areas of biotechnology, and finally (4) discussing about the development strategies and the future perspectives on poorly studied extremophilic microorganisms in the country to fulfill future biotechnological and industrial demands.

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References

  • Acer O, Pirinccioglu H, Matpan Bekler F, Gul Guven R, Guven K (2015) Anoxybacillus sp. AH1, an amylase producing thermophilic bacterium isolated from Dargecit hot spring. Biologia 70(7):853–862

    Article  CAS  Google Scholar 

  • Acer O, Matpan Bekler F, Pirinccioglu H, Gul Guven R, Guven K (2016) Purification and characterization of thermostable and detergent-stable α-amylase from Anoxybacillus sp. AH1. Food Technol Biotechnol 54(1):70–77

    PubMed  PubMed Central  CAS  Google Scholar 

  • Acikgoz E, Ozcan B (2016) Phenol biodegradation by halophilic archaea. Int Biodeterior Biodegrad 107:140–146

    Article  CAS  Google Scholar 

  • Adiguzel A, Ozkan H, Baris O, Inan K, Gulluce M, Sahin F (2009) Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. J Microbiol Methods 79:321–328

    Article  CAS  PubMed  Google Scholar 

  • Adiguzel A, Inan K, Sahin F, Arasoglu T, Gulluce M (2011) Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey). Turk J Biol 35(3):267–274

    CAS  Google Scholar 

  • Ahmetoglu N, Matpan Bekler F, Acer O, Guven G, Guven K (2015) Production, purification and characterisation of thermostable metallo-protease from newly isolated Bacillus sp. KG5. Eurasia J Biosci 9:1–11

    Article  CAS  Google Scholar 

  • Alkan H, Gul Guven R, Guven K, Erdogan S, Dogru M (2015) Biosorption of Cd2+, Cu2+, and Ni2+ ions by a thermophilic haloalkalitolerant bacterial strain (KG9) immobilized on amberlite XAD-4. Pol J Environ Stud 24(5):1903–1910

    Article  CAS  Google Scholar 

  • Arikan B, Unaldi N, Coral G, Colak O, Aygan A, Gulnaz O (2003) Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochem 38:1397–1403

    Article  CAS  Google Scholar 

  • Atasoy P, Canakci S, Belduz AO (2012) Purification, characterization and industrial application of the alkaline protease (akap) from Anoxybacillus kestanbolensis K4. New Biotechnol 29S:118

    Article  Google Scholar 

  • Ates O, Arga KY, Toksoy Oner E (2013) The stimulatory effect of mannitol on levan biosynthesis: lessons from metabolic systems analysis of Halomonas smyrnensis AAD6. Biotechnol Prog 29(6):1386–1397

    Article  PubMed  CAS  Google Scholar 

  • Ay F, Karaoglu H, Inan K, Canakci S, Belduz AO (2011) Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1. Protein Expr Purif 80:74–79

    Article  PubMed  CAS  Google Scholar 

  • Bakir Ateslier ZB, Metin K (2006) Production and partial characterization of a novel thermostable esterase from a thermophilic Bacillus sp. Enzyme MicrobTechnol 38(5):628–635

    Article  CAS  Google Scholar 

  • Bakir ZB, Metin K (2015) Screening for industrially important enzymes from thermophilic bacteria; selection of lipase-producing microorganisms and optimization of culture conditions. European J Biotechnol Biosci 3(6):43–48

    Google Scholar 

  • Bakir ZB, Metin K (2017) Production and characterization of an alkaline lipase from thermophilic Anoxybacillus sp. HBB16. Chem Biochem Eng Q 31(3):303–312

    Article  CAS  Google Scholar 

  • Baltaci MO, Genc B, Arslan S, Adiguzel G, Adiguzel A (2016) Isolation and characterization of thermophilic bacteria from geothermal areas in Turkey and preliminary research on biotechnologically important enzyme production. Geomicrobiol J 34(1):53–62

    Article  CAS  Google Scholar 

  • Baltas N, Dincer B, Kolayli S, Ekinci AP, Adiguzel A, Gulluce M (2012) Purification and characterization of extracellular alpha-amylase from Anoxybacillus gonensis Z4 strain. Turk J Biochem 37(1):140

    Google Scholar 

  • Baltas N, Dincer B, Ekinci AP, Kolayli S, Adiguzel A (2016) Purification and characterization of extracellular α-amylase from a thermophilic Anoxybacillus thermarum A4 strain. Braz Arch Biol Technol 59:e16160346

    Article  CAS  Google Scholar 

  • Belduz AO, Dulger S, Demirbag Z (2003) Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53:1315–1320

    Article  PubMed  CAS  Google Scholar 

  • Belduz AO, Canakci S, Chan KG et al (2015) Genome sequence of Anoxybacillus ayderensis AB04T isolated from the Ayder hot spring in Turkey. Stand Genomic Sci 10:70

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Beris FS, De Smet L, Karaoglu H, Canakci S, Van Beemuen J, Belduz AO (2011) The ATPase activity of the G2alt gene encoding an aluminium tolerance protein from Anoxybacillus gonensis G2. J Microbiol 49:641–650

    Article  PubMed  CAS  Google Scholar 

  • Birbir M, Sesal C (2003) Extremely halophilic bacterial communities in Sereflikochisar Salt Lake in Turkey. Turk J Biol 27(7):7–21

    Google Scholar 

  • Birbir M, Ogan A, Calli B, Mertoglu B (2004) Enzyme characteristics of extremely halophilic archaeal community in Tuzkoy Salt Mine, Turkey. World J Microbiol Biotechnol 20:613–621

    Article  CAS  Google Scholar 

  • Birbir M, Calli B, Mertoglu B, Bardavid RE, Oren A, Ogmen MN, Ogan A (2007) Extremely halophilic archaea from Tuz Lake, Turkey, and the adjacent Kaldirim and Kayacik salterns. World J Microbiol Biotechnol 23:309–316

    Article  CAS  Google Scholar 

  • Bozoglu C, Adiguzel A, Nadaroglu H, Yanmis D, Gulluce M (2013) Purification and characterization of laccase from a newly isolated thermophilic Brevibacillus sp. (Z1) and its application of removal textile dyes. Res J Biotechnol 8(9):56–66

    CAS  Google Scholar 

  • Caglayan M, Bilgin N (2011) Cloning and sequences analysis of novel DNA polymerases from thermophilic Geobacillus species isolated from hot springs in Turkey: characterization of a DNA polymerase I from Geobacillus kaue strain NB. Appl Biochem Biotechnol 165:1188–1200

    Article  PubMed  CAS  Google Scholar 

  • Caglayan M, Bilgin N (2012) Temperature dependence of accuracy of DNA polymerase I from Geobacillus anatolicus. Biochimie 94(9):1968–1973

    Article  PubMed  CAS  Google Scholar 

  • Cakmak U, Saglam Ertunga N (2017) Gene cloning, expression, immobilization and characterization of endo-xylanase from Geobacillus sp. TF16 and investigation of its industrial applications. J Mol Catal B: Enzym 133:288–298

    Article  CAS  Google Scholar 

  • Calimlioglu B, Arga KY (2016) Proteins from halophilic bacteria: purification and their applications. In: Protein purification: principles and trends. iConcept Press Hong Kong isbn:978-1-922227-40-9

    Google Scholar 

  • Canakci S, Inan K, Kacagan M, Belduz AO (2007) Evaluation of arabinofuranosidase and xylanase activities of Geobacillus spp. isolated from some hot springs in Turkey. J Microbiol Biotechnol 17:1261–1270

    Google Scholar 

  • Canakci S, Cevher Z, Inan K, Tokgoz M, Bahar F, Kacagan M, Fulya AS, Belduz AO (2012) Cloning, purification and characterization of an alkali-stable endoxylanase from thermophilic Geobacillus sp. 71. World J Microbiol Biotechnol 28:1981–1988

    Article  PubMed  CAS  Google Scholar 

  • Cihan AC (2013) Taxonomic classification of Anoxybacillus isolates from geothermal regions in Turkey by 16S rRNA gene sequences and ARDRA, ITS-PCR, Rep-PCR analyses. Pol J Microbiol 62(2):149–163

    PubMed  CAS  Google Scholar 

  • Cihan AC, Yildiz ED (2016) General characteristics, taxonomic studies and biotechnological importance of the genus Anoxybacillus. Commun Fac Sci Univ Ank Series C 25(1-2):56–82

    Google Scholar 

  • Cihan A, Ozcan B, Cokmus C (2009) Characterization of thermostable glucosidases from newly isolated Geobacillus sp. A333 and thermophilic bacterium A343. World J Microbiol Biotechnol 25:2205–2217

    Article  CAS  Google Scholar 

  • Cihan AC, Ozcan B, Cokmus C (2011a) Anoxybacillus salavatliensis sp. nov., an α-glucosidase producing, thermophilic bacterium isolated from Salavatli, Turkey. J Basic Microbiol 51(2):136–146

    Article  CAS  PubMed  Google Scholar 

  • Cihan AC, Ozcan B, Tekin N, Cokmus C (2011b) Geobacillus thermodenitrificans subsp. calidus, subsp. nov., a thermophilic and α-glucosidase producing bacterium isolated from Kizilcahamam, Turkey. J Gen Appl Microbiol 57(2):83–92

    Article  PubMed  CAS  Google Scholar 

  • Cihan AC, Cokmus C, Koc M, Ozcan B (2014) Anoxybacillus calidus sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant. Int J Syst Evol Microbiol 64:211–219

    Article  PubMed  Google Scholar 

  • Colak A, Sisik D, Saglam N, Guner S, Canakci S, Belduz AO (2005) Characterization of a thermoalkalophilic esterase from a novel thermophilic bacterium, Anoxybacillus gonensis G2. Bioresour Technol 96:625–631

    Article  CAS  PubMed  Google Scholar 

  • Colak A, Col M, Canakci S, Belduz AO, Omarov I (2008) Investigation of extracellular highly thermostable starch hydrolyzing activity from a novel thermophilic bacterium Anoxybacillus gonensis A4. Asian J Chem 20:1577–1587

    CAS  Google Scholar 

  • Coleri A, Cokmus C, Ozcan B, Akkoc N, Akcelik M (2009) Isolations of α-glucosidase-producing thermophilic bacilli from hot springs of Turkey. Microbiology 78(1):56–66

    Article  CAS  Google Scholar 

  • Da Mota FF, Gomes EA, Paiva E, Rosado AS, Seldin L (2004) Use of rpoB gene analysis for identification of nitro-genfixing Paenibacillus species as an alternative to the 16S rRNA gene. Lett Appl Microbiol 39:34–40

    Article  PubMed  CAS  Google Scholar 

  • DasSarma S, DasSarma P (2017) Halophiles. In: eLS. Wiley, Chichester. http://www.els.net. https://doi.org/10.1002/9780470015902.a0000394.pub4

  • Demirci A, Mutlu MB, Guven A, Korcan E, Guven K (2011) Decolorization of textile azo-metal complex dyes by a halophilic bacterium isolated from Camalti Saltern in Turkey. CLEAN 39(2):177–184

    CAS  Google Scholar 

  • Demirjian DC, Moris-Varas F, Cassidy CS (2001) Enzymes from extremophiles. Curr Opin Chem Biol 5:144–151

    Article  CAS  PubMed  Google Scholar 

  • Dulger S, Demirbag Z, Belduz AO (2004) Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov. Int J Syst Evol Microbiol 54:1499–1503

    Article  PubMed  CAS  Google Scholar 

  • Duran C, Bulut VN, Gundogdu A, Soylak M, Belduz AO, Beris FS (2009) Biosorption of heavy metals by Anoxybacillus gonensis immobilized on diaion HP-2MG. Sep Sci Technol 44(2):335–358

    Article  CAS  Google Scholar 

  • Ekinci AP, Dincer B, Baltas N, Adiguzel A (2016) Partial purification and characterization of lipase from Geobacillus stearothermophilus AH22. J Enzyme Inhib Med Chem 31(2):325–331

    Article  CAS  PubMed  Google Scholar 

  • Elevi R, Assa P, Birbir M, Ogan A, Oren A (2004) Characterization of extremely halophilic Archaea isolated from the Ayvalik Saltern, Turkey. World J Microbiol Biotechnol 20:719–725

    Article  CAS  Google Scholar 

  • Erdogmus SF, Mutlu B, Korcan SE, Guven K, Konuk M (2013) Aromatic hydrocarbon degradation by halophilic archaea isolated from Camalti Saltern, Turkey. Water Air Soil Pollut 224:1449

    Article  CAS  Google Scholar 

  • Ertunga NS, Colak A, Belduz AO, Canakci S, Karaoglu H, Sandalli C (2007) Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2. J Biochem 141:817–825

    Article  CAS  PubMed  Google Scholar 

  • Faiz O, Colak A, Saglam N, Canakci S, Belduz AO (2007) Determination and characterization of thermostable esterolytic activity from a novel thermophilic bacterium Anoxybacillus gonensis A4. J Biochem Mol Biol 40:588–594

    CAS  PubMed  Google Scholar 

  • Fincan SA, Enez B, Ozdemir S, Matpan Bekler F (2014) Purification and characterization of thermostable α-amylase from thermophilic Anoxybacillus flavithermus. Carbohydr Polym 102:144–150

    Article  CAS  Google Scholar 

  • Genc B, Baltaci O, Nadaroglu H, Adiguzel A (2014) A novel cellulase from Anoxybacillus gonensis A4 isolated from geothermal area in Turkey. In: Molecular biology conference, 10–12 September, 2014, Turkey, p 47

    Google Scholar 

  • Genc B, Nadaroglu H, Adiguzel A, Baltaci O (2015) Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey. J Environ Biol 36:1319–1324

    PubMed  CAS  Google Scholar 

  • Goh KM, Kahar UM, Chai YY, Chong CS, Chai KP, Ranjani V, Illias RM, Chan KG (2013) Recent discoveries and applications of Anoxybacillus. Appl Microbiol Biotechnol 97:1475–1488

    Article  CAS  PubMed  Google Scholar 

  • Grant WD, Kamekura M, McGenity TJ, Ventosa A (2001) Order I. Halobacteriales. In: Garrity GM (ed) Bergey’s manual of systematic bacteriology V.I, the Archaea and deeply branching and phototrophic bacteria, 2nd edn. Springer, New York, p 294–333. http://turkeyculture.org/

  • Gul Guven R (2007) Isolation and identification of bacteria from hot springs, and β-galactosidase purification from Alicyclobacillus acidocaldarius subsp. rittmanii. PhD thesis, Dicle University, Diyarbakir

    Google Scholar 

  • Gul Guven R, Guven K, Poli A, Nicolaus B (2008) Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov., a thermophilic bacterium isolated from a hot spring in Batman. J Gen Appl Microbiol 54:337–354

    Article  Google Scholar 

  • Gutiérrez-Arnillas E, Rodríguez A, Sanromán MA, Deive FJ (2016) New sources of halophilic lipases: Isolation of bacteria from Spanish and Turkish saltworks. Biochem Eng J 109:170–177

    Article  CAS  Google Scholar 

  • Inan K, Belduz AO, Canakci S (2011a) Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turk J Biol 35:529–542

    CAS  Google Scholar 

  • Inan K, Bektas Y, Canakci S, Belduz AO (2011b) Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species. J Microbiol 49:782–790

    Article  PubMed  Google Scholar 

  • Inan K, Canakci S, Belduz AO, Sahin F (2012) Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring. Int J Syst Evol Microbiol 62:849–855

    Article  CAS  PubMed  Google Scholar 

  • Inan K, Belduz AO, Canakci S (2013) Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium. J Basic Microbiol 53:410–419

    Article  PubMed  CAS  Google Scholar 

  • Inan K, Ozer A, Guler HI, Belduz AO, Canakci S (2016) Brevibacillus gelatini sp. nov., isolated from a hot spring. Int J Syst Evol Microbiol 66:712–718

    Article  CAS  PubMed  Google Scholar 

  • Kacagan M, Canakci S, Sandalli C, Inan K, Colak DN, Belduz AO (2008) Characterization of a xylanase from a thermophilic strain of Anoxybacillus pushchinoensis A8. Biologia 63:599–606

    Article  CAS  Google Scholar 

  • Kacagan M, Inan K, Canakci S, Guler HI, Belduz AO (2015) Thermus anatoliensis sp. nov., a thermophilic bacterium from geothermal waters of Buharkent, Turkey. J Basic Microbiol 55:1367–1373

    Article  CAS  PubMed  Google Scholar 

  • Karadag D, Makinen AE, Efimova E, Puhakka JA (2016) Thermophilic biohydrogen production by an anaerobic heat treated-hot spring culture. Bioresour Technol 100:5790–5795

    Article  CAS  Google Scholar 

  • Karaoglu H, Yanmis D, Ay F, Celik A, Canakci S, Belduz AO (2013) Biochemical characterization of a novel glucose isomerase from Anoxybacillus gonensis G2T that displays a high level of activity and thermal stability. J Mol Catal B Enzym 97:215–224

    Article  CAS  Google Scholar 

  • Kargi F, Dincer AR (1998) Saline wastewater treatment by halophile-supplemented activated sludge culture in an aerated rotating biodisc contactor. Enzym Microb Technol 22:427–433

    Article  CAS  Google Scholar 

  • Kocabiyik S, Erdem B (2002) Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR). Bioresour Technol 84:29–33

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Karan R, Kapoor S, Singh SP, Khare SK (2012) Screening and isolation of halophilic bacteria producing industrially important enzymes. Braz J Microbiol 43:1595–1603

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kurt Kizildogan A, Abanoz B, Okay S (2017) Global transcriptome analysis of Halolamina sp. to decipher the salt tolerance in extremely halophilic archaea. Gene 601:56–64

    Article  CAS  PubMed  Google Scholar 

  • Lim YL, Chan KG, Ee R, Belduz AO, Canakci S, Kahar UM, Yaakop AS, Goh KM (2015) Complete genome of the potential thermozyme producer Anoxybacillus gonensis G2T isolated from the Gonen hot springs in Turkey. J Biotechnol 212:65–66

    Article  CAS  PubMed  Google Scholar 

  • Loder AJ, Zeldes B, Conway JM, Counts JA, Straub CT, Khatibi PA, Lee LL, Vitko NP, Keller M, Rhaesa AM, Rubinstein GM, Scott IM, Lipscomb GL, Adams MWW, Kelly M (2017) Extreme thermophiles as metabolic engineering platforms: strategies and current perspective. In: Wittmann C, Liao JC (eds) Industrial biotechnology: microorganisms. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. https://doi.org/10.1002/9783527807796.ch14

    Chapter  Google Scholar 

  • Madern D, Ebel C, Zaccai G (2000) Halophilic adaptation of enzymes. Extremophiles 4:91–98

    Article  CAS  PubMed  Google Scholar 

  • Mandic Mulec I, Stefanic P, van Elsas J (2016) Ecology of Bacillaceae. In: Driks A, Eichenberger P (eds) The bacterial spore: from molecules to systems. ASM Press, Washington, DC, pp 59–85

    Google Scholar 

  • Matpan Bekler F (2016) Isolation and identification of a novel thermo-alkalophilic Anoxybacillus sp. strain KB4 from Kusburnu hot spring in Turkey. BioDiCon 9(2):169–179

    Google Scholar 

  • Matpan Bekler F (2017) Physio-biochemical characterization and 16S rRNA sequencing of thermophilic Geobacillus sp. strain DB2 from Dibekli hot spring, Turkey. IÄŸdır Univ J Inst Sci Tech 7(1):63–71

    Article  Google Scholar 

  • Matpan Bekler F, Guven K (2014) Isolation and production of thermostable α-amylase from thermophilic Anoxybacillus sp. KP1 from Diyadin hot spring in AÄŸri. Turkey Biologia 69(4):419–427

    CAS  Google Scholar 

  • Matpan Bekler F, Stougaard P, Guven RG, Guven K, Acer O (2015a) Isolation and cloning of extracellular thermostable β-galactosidases from a newly isolated thermophilic Bacillus licheniformis KG9. Cell Mol Biol 61(3):71–78

    CAS  PubMed  Google Scholar 

  • Matpan Bekler F, Acer O, Guven K (2015b) Co-Production of thermostable, calcium-independent α-amylase and alkali-metallo protease from newly isolated Bacillus licheniformis DV3. Innov Rom Food Biotechnol 16:21–30

    Google Scholar 

  • Matpan Bekler F, Acer O, Guven K (2015c) Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1. Cell Mol Biol 61(4):118–125

    Google Scholar 

  • Matpan Bekler F, Yalaz S, Acer O, Guven K (2017) Purification of thermostable β-galactosidase from Anoxybacillus sp. KP1 and estimation of combined effect of some chemicals on enzyme activity using semiparametric errors in variables model. Fresenius Environ Bull 26(3):2253–2261

    Google Scholar 

  • Mehta R, Singhal P, Singh H, Damle D, Sharma AK (2016) Insight into thermophiles and their wide-spectrum applications. 3. Biotech 6(1):81

    Google Scholar 

  • Mutlu MB, Guven K (2011) Detection of prokaryotic microbial communities of Çamaltı Saltern, Turkey, by fluorescein in situ hybridization and real-time PCR. Turk J Biol 35:687–695

    CAS  Google Scholar 

  • Mutlu MB, Guven K (2015) Bacterial diversity in Camalti Saltern, Turkey. Pol J Microbiol 64(1):37–45

    PubMed  Google Scholar 

  • Mutlu MB, Martinez-Garcia M, Santos F, Pena A, Guven K, Anton J (2008) Prokaryotic diversity in Tuz Lake, a hypersaline environment in Inland Turkey. FEMS Microbiol Ecol 65:474–483

    Article  PubMed  CAS  Google Scholar 

  • Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE, Lysenko AM, Petrunyaka VV, Osipov GA, Belyaev SS, Ivanov MV (2001) Taxonomic study of aerobic thermophilic bacilli: Descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int J Syst Evol Microbiol 51:433–446

    Article  PubMed  CAS  Google Scholar 

  • Olson DG, Sparling R, Lynd LR (2015) Ethanol production by engineered thermophiles. Curr Opin Biotechnol 33:130–141

    Article  PubMed  CAS  Google Scholar 

  • Oren A (2010) Industrial and environmental applications of halophilic microorganisms. Environ Technol 31(8-9):825–834

    Article  PubMed  CAS  Google Scholar 

  • Orhan F, Gulluce M (2015) Isolation and characterization of salt-tolerant bacterial strains in salt-affected soils of Erzurum, Turkey. Geomicrobiol J 32(6):521–529

    Article  CAS  Google Scholar 

  • Ozcan B, Ozcengiz G, Coleri A, Cokmus C (2007) Diversity of halophilic archaea from six hypersaline environments in Turkey. J Microbiol Biotechnol 17(5):745–752

    Google Scholar 

  • Ozcan B, Ozyilmaz G, Cokmus C, Caliskan M (2009) Characterization of extracellular esterase and lipase activities from five halophilic archaeal strains. J Ind Microbiol Biotechnol 36:105–110

    Article  PubMed  CAS  Google Scholar 

  • Ozdemir S, Matpan F, Guven K, Baysal Z (2011) Production and characterization of partially purified extracellular thermostable α-amylase by Bacillus subtilis in submerged fermentation (SmF). Prep Biochem Biotechnol 41:365–381

    Article  PubMed  CAS  Google Scholar 

  • Ozdemir S, Matpan F, Okumus V, Dundar A, Ulutas MS, Kumru M (2012) Isolation a thermophilic Anoxybacillus flavithermus sp. nov. and production of thermostable α-amylase under solid-state fermentation (SSF). Ann Microbiol 62:1367–1375

    Article  CAS  Google Scholar 

  • Oztekin Z, Ay F, Karaoglu H, Colak DN, Belduz AO (2011) Purification and characterization of W137F/V184T double mutations on Anoxybacillus gonensis G2 xylose isomerase. Curr Opin Biotechnol 22:87

    Article  Google Scholar 

  • Ozturk HU, Sariyar Akbulut B, Ayan B, Poli A, Denizci AA, Utkan G, Nicolaus B, Kazan D (2015) Moderately halophilic bacterium Halomonas sp. AAD12: a promising candidate as a hydroxyectoine producer. J Microb Biochem Technol 7:262–268

    CAS  Google Scholar 

  • Pikuta E, Lysenko A, Chuvilskaya N, Mendrock U, Hippe H, Suzina N, Nikitin D, Osipov G, Laurinavichius K (2000) Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50:2109–2117

    Article  PubMed  CAS  Google Scholar 

  • Poli A, Kazak H, Gurleyendag B, Tommonaro G, Pieretti G, Oner ET, Nicolaus B (2009) High level synthesis of levan by a novel Halomonas species growing on defined media. Carbohydr Polym 78:651–657

    Article  CAS  Google Scholar 

  • Poli A, Laezza G, Gul Guven R, Orlando P, Nicolaus B (2011) Geobacillus galactosidasius sp. nov., a new thermophilic galactosidase producing bacterium isolated from compost. Syst Appl Microbiol 34:419–423

    Article  PubMed  CAS  Google Scholar 

  • Poli A, Guven K, Romano I, Pirinccioglu H, Gul Guven R, Euzeby JP, Matpan F, Acer O, Orlando P, Nicolaus B (2012) Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey. J Gen Appl Microbiol 58:437–446

    Article  PubMed  CAS  Google Scholar 

  • Poli A, Nicolaus B, Denizci AA, Yavuzturk B, Kazan D (2013) Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 63:10–18

    Article  PubMed  Google Scholar 

  • Sam S, Kucukasik F, Yenigun O, Nicolaus B, Toksoy Oner E, Yukselen MA (2011) Flocculating performances of exopolysaccharides produced by a halophilic bacterial strain cultivated on agro-industrial waste. Bioresour Technol 102(2):1788–1794

    Article  PubMed  CAS  Google Scholar 

  • Sandalli C, Kacagan M, Canakci S, Belduz AO (2008) Cloning, expression, purification and characterisation of a thermostable chitinase from Bacillus licheniformis A1. Ann Microbiol 58:245

    Article  CAS  Google Scholar 

  • Sandalli C, Saral A, Ulker S, Karaoglu H, Belduz AO, Copur Cicek A (2014) Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turk J Biol 38:111–117

    Article  CAS  Google Scholar 

  • Shida O, Takagi H, Kadowaki K, Komagata K (1996) Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946

    Article  PubMed  CAS  Google Scholar 

  • Sirin Y, Yildirim Akatin M, Colak A, Saglam Ertunga N (2017) Dephytinization of food stuffs by phytase of Geobacillus sp. TF16 immobilized in chitosan and calcium-alginate. Int J Food Prop. https://doi.org/10.1080/10942912.2016.1261151

  • Sogutcu E, Emrence Z, Arikan M, Cakiris A, Abaci N, Toksoy Oner E, Ustek D, Arga KY (2012) Whole genome sequencing, assembly and annotation of Halomonas smyrnensis, a levan producing halophilic bacterium. EMBnet.journal 18.A. POSTERS. 89

    Google Scholar 

  • Souza PMD (2010) Application of microbial α-amylase in industry-A review. Braz J Microbiol 41(4):850–861

    Article  PubMed  PubMed Central  Google Scholar 

  • Taslimi P, Adiguzel A, Nadaroglu H, Bozoglu C, Gulluce M (2013) Removal of some textile dyes from aqueous solution by using a catalase-peroxidase from Aeribacillus pallidus (P26). J Pure Appl Microbiol 7(4):2629–2640

    CAS  Google Scholar 

  • Tatar D, Guven K, Inan K, Cetin D, Belduz AO, Sahin N (2016) Streptomonospora tuzyakensis sp. nov., a halophilic actinomycete isolated from saline soil. Antonie Van Leeuwenhoek 109(1):35–41

    Article  PubMed  CAS  Google Scholar 

  • Taylor MP, Eley KL, Martin S, Tuffin MI, Burton SG, Cowan DA (2009) Thermophilic ethanologenesis: future prospects for second-generation bioethanol production. Trends Biotechnol 27(7):398–405

    Article  CAS  PubMed  Google Scholar 

  • Tokgoz M, Inan K, Belduz AO, Gedikli O, Canakci S (2014) Cloning, purification, and characterization of a thermophilic ribulokinase from Anoxybacillus kestanbolensis AC26Sari. Turk J Biol 38:633–639

    Article  CAS  Google Scholar 

  • Turner P, Mamo G, Nordberg Karlsson E (2007) Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microb Cell Factories 6:9. https://doi.org/10.1186/1475-2859-6-9

    Article  CAS  Google Scholar 

  • Uzyol KS, Sariyar Akbulut B, Denizci AA, Kazan D (2012) Thermostable α-amylase from moderately halophilic Halomonas sp. AAD21. Turk J Biol 36:327–338

    CAS  Google Scholar 

  • Vargas C, Nieto JJ (2004) Genetic tools for the manipulation of moderately halophilic bacteria of the family Halomonadaceae. In: Balbás P, Lorence A (eds) Methods in Molecular Biology, Recombinant Gene Expression: Reviews and Protocols, vol 267, 2nd edn. Humana Press Inc., Totowa, pp 183–208

    Google Scholar 

  • Ventosa A, Márquez MC, Garabito MJ, Arahal DR (1998) Moderately halophilic gram-positive bacterial diversity in hypersaline environments. Extremophiles 2:297–304

    Article  CAS  PubMed  Google Scholar 

  • Yanmis D, Baltaci MO, Gulluce M, Adiguzel A (2015) Identification of thermophilic strains from geothermal areas in Turkey by using conventional and molecular techniques. Res J Biotechnol 10(1):39–45

    CAS  Google Scholar 

  • Yanmis D, Adiguzel A, Nadaroglu H, Gulluce M, Demir N (2016) Purification and characterization of laccase from thermophilic Anoxybacillus gonensis P39 and its application of removal textile dyes. Rom Biotechnol Lett 21:11485–11496

    CAS  Google Scholar 

  • Yavuz E, Gunes H, Harsa S, Yenidunya AF (2004) Identification of extracellular enzyme producing thermophilic bacilli from Balcova (Agamemnon) geothermal site by ITS rDNA RFLP. J Appl Microbiol 97:810–817

    Article  CAS  PubMed  Google Scholar 

  • Yener I, Varhan Oral E, Dolak I, Ozdemir S, Ziyadanogullari R (2017) A new method for preconcentration of Th(IV) and Ce(III) by thermophilic Anoxybacillus flavithermus immobilized on Amberlite XAD-16 resin as a novel biosorbent. Ecol Eng 103:43–49

    Article  Google Scholar 

  • Yildirim V, Baltaci MO, Ozgencli I, Sisecioglu M, Adiguzel A, Adiguzel G (2017) Purification and biochemical characterization of a novel thermostable serine alkaline protease from Aeribacillus pallidus C10: a potential additive for detergents. J Enzyme Inhib Med Chem 32(1):468–477

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Yilmaz B, Baltaci MO, Yildirim V, Sisecioglu M, Adiguzel A (2014) Purification and Characterization of Thermostable Alkaline Protease Enzyme from Bacillus licheniformis A10. In: MolBiolCon2014. 10–12 September/Turkey, p48

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Dicle University, Diyarbakir, Turkey

    Kemal Guven

  2. Department of Biology, Dicle University, Diyarbakir, Turkey

    Fatma Matpan Bekler

  3. Department of Science and Mathematics Education, Dicle University, Diyarbakir, Turkey

    Reyhan Gul Guven

Authors
  1. Kemal Guven
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  2. Fatma Matpan Bekler
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  3. Reyhan Gul Guven
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Correspondence to Kemal Guven .

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Editors and Affiliations

  1. Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan

    Dilfuza Egamberdieva

  2. Department of Biology, University of Bergen, Bergen, Norway

    Nils-KÃ¥re Birkeland

  3. Faculty of Biology, Yerevan State University, Yerevan, Armenia

    Hovik Panosyan

  4. School of Life Sciences, Sun Yat-Sen University, Guangzhou, China

    Wen-Jun Li

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Guven, K., Matpan Bekler, F., Gul Guven, R. (2018). Thermophilic and Halophilic Microorganisms Isolated from Extreme Environments of Turkey, with Potential Biotechnological Applications. In: Egamberdieva, D., Birkeland, NK., Panosyan, H., Li, WJ. (eds) Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications. Microorganisms for Sustainability, vol 8. Springer, Singapore. https://doi.org/10.1007/978-981-13-0329-6_8

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