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The Synergistic Antibacterial Effect of Azadirachta indica Leaves Extract and Aloe barbadensis Gel Against Bacteria Associated with Skin Infection

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Abstract

Combinations of two agents which create inhibitory effects that are greater than the individual effects produce a positive interaction known as synergism. This method can help in developing agents for antibacterial activity in order to treat bacterial infection. This study was done to assess the possible synergistic antibacterial effect of the combination between Azadirachta indica leaves extracts and Aloe barbadensis gel against five bacteria commonly associated with skin infection. Synergistic antibacterial activities from the interaction of both plants against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella aerogenes and Streptococcus faecalis were measured by using Kirby–Bauer disc diffusion assay. A. indica ethanolic extract, A. barbadensis gel, combination of A. indica ethanolic extract and A. barbadensis gel, and commercialize antibiotics were tested on the five bacteria. The data were subjected to statistical analysis of one-way ANOVA and Tukey’s post hoc tests (α = 0.05). The results of this study showed significant inhibition of the bacteria from the synergistic effect of A. indica and A. barbadensis compared to the commercial antibiotic. The significant results may contribute to the development of stronger antibacterial agent in skin infection treatment in the pharmaceutical industry.

Keywords

  • Azadirachta indica
  • Aloe barbadensis
  • Synergistic antibacterial activity
  • Kirby–Bauer disc diffusion assay versus skin infection bacteria

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Acknowledgements

We would like to thank Universiti Teknologi MARA Perlis Branch for providing the laboratory facilities to conduct the research project.

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Correspondence to Hamidah Jaafar Sidek .

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Sidek, H.J., Azman, M.A., Sharudin, M.S.M. (2018). The Synergistic Antibacterial Effect of Azadirachta indica Leaves Extract and Aloe barbadensis Gel Against Bacteria Associated with Skin Infection. In: Saian, R., Abbas, M. (eds) Proceedings of the Second International Conference on the Future of ASEAN (ICoFA) 2017 – Volume 2. Springer, Singapore. https://doi.org/10.1007/978-981-10-8471-3_58

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  • DOI: https://doi.org/10.1007/978-981-10-8471-3_58

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