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Functional Mechanisms of ABC Transporters as Revealed by Molecular Simulations

  • Tadaomi Furuta
  • Minoru Sakurai
Chapter

Abstract

Active transport in cells is accomplished by a class of integral membrane proteins known as ATP-binding cassette (ABC) transporters. The energy source powering these molecular machines is the free energy generated by the binding of ATP molecules to nucleotide-binding domains (NBDs), as well as the free energy generated by ATP hydrolysis. The opening and closing motions of the NBDs are driven by these energies, which are propagated through transmembrane domains (TMDs) via mechanical transmission segments (coupling helices). As a result, the opening and closing motions of the TMDs are generated, which allow the uptake and release of substrates. In these processes, the chemical energy of ATP is converted into mechanical motion, a typical example of chemo-mechanical coupling. In this review, we describe the current understanding of this coupling mechanism, with a focus on the cooperative role of ATP and water.

Keywords

ATP Water Chemo-mechanical coupling ATPase 

Notes

Acknowledgements

This work was supported in part by JSPS KAKENHI JP16H00825, JP16K12520, and JP15K00400. We cordially thank Mr. Sho Tanaka for his contribution to the calculated data shown in Tables 12.1 and 12.2.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Center for Biological Resources and InformaticsTokyo Institute of TechnologyTokyoJapan

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