Generation of Aptamers Against Natural Toxins and Their Application as Biosensors
RNA or ssDNA aptamers, which are capable of binding to target molecules with high affinity and specificity, are selected in vitro from large combinatorial nucleic acid libraries by a process known as systematic evolution of ligands by exponential enrichment (SELEX). Using this SELEX technology, many aptamers have been generated against a wide range of target molecules, including proteins, nucleic acids, small molecules, and whole cells. In this chapter, we described various methods for generating aptamers, including methods that do not require target immobilization. Among these aptamers, ones that are specific for natural toxins, such as mycotoxins, are of great interest to the food industry, as they can used in developing tools (biosensors) for ensuring food safety. We also summarized several aptamer-based detection strategies. Lastly, we described biosensor applications of aptamers for natural toxins.
KeywordsRNA Aptamer SELEX Aptasensor Natural toxin
We thank Dr. Yasuyuki Tomita for helpful discussions.
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