Engineered Group I Ribozymes as RNA-Based Modular Tools to Control Gene Expression
Group I ribozymes (RNA enzymes) constitute a class of structural RNAs that promote cleavage and ligation of phosphodiester bonds to conduct RNA splicing. As naturally occurring group I ribozymes are constitutively active in most cases, modular engineering is required to apply them to control gene expression. We first introduce exceptional examples of modified splicing reactions of group I ribozymes. We then summarize strategies to engineer group I ribozymes to develop genetic modular tools and also their application to gene expression control.
KeywordsAlternative splicing Aptamer Group I intron Ribozyme RNA structure Self-splicing Trans-splicing
This work was supported by JSPS KAKENHI Grant Number JP 15 K05561 (to Y.I.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. This work was also supported partly by University of Toyama Discretionary Funds of the President “Toyama RNA Research Alliance” (to Y.I. and S.M.).
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