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Basic and Advanced Laboratory Techniques in Histopathology and Cytology pp 113–118Cite as

Stains for the Microbial Organisms

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Abstract

Microbial organisms are sub-microscopic infective organisms that usually produce diseases. They are subdivided into (1) bacteria, (2) fungi, (3) protozoa, (4) helminths and (5) virus. It is not always possible to identify the microbes by routine haematoxylin and eosin stain, and often special stains are required to demonstrate the morphology and characteristic colour of the organisms. This chapter describes the essential stains to demonstrate the various microorganisms in the histopathology and cytology material. The principle of stains, basic ingredients of the staining solution and the steps of staining have been described here.

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References

  1. Woods GL, Walker DH. Detection of infection or infectious agents by use of cytologic and histologic stains. Clin Microbiol Rev. 1996;9(3):382–404.

    PubMed  PubMed Central  CAS  Google Scholar 

  2. Kwon-Chung KJ, Hill WB, Bennett JE. New, special stain for histopathological diagnosis of cryptococcosis. J Clin Microbiol. 1981;13(2):383–7.

    PubMed  PubMed Central  CAS  Google Scholar 

  3. Madison BM. Application of stains in clinical microbiology. Biotech Histochem. 2001;76(3):119–25.

    Article  CAS  PubMed  Google Scholar 

  4. Gram HC. Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten. Fortschr Med. 1884;2:185–9.

    Google Scholar 

  5. Engbaek K, Johansen KS, Jensen ME. A new technique for Gram staining paraffin-embedded tissue. J Clin Pathol. 1979;32(2):187–90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Wade HW. A modification of the Fite formaldehyde (Fite I) method for staining acid-fast bacilli in paraffin sections. Stain Technol. 1957;32(6):287–92.

    Article  CAS  PubMed  Google Scholar 

  7. Grocott RG. A stain for fungi in tissue sections and smears using Gomori's methenamine-silver nitrate technique. Am J Clin Pathol. 1955;25:975–9.

    Article  CAS  PubMed  Google Scholar 

  8. Warthin AS, Chronister AC. A more rapid and improved method of demonstrating spirochetes in tissues (Warthin and Starry’s cover-glass method). American Journal of Syphilis. 1920;4:97–103.

    Google Scholar 

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Authors and Affiliations

  1. Education and Research (PGIMER), Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

    Pranab Dey

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  1. Pranab Dey
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Dey, P. (2018). Stains for the Microbial Organisms. In: Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Springer, Singapore. https://doi.org/10.1007/978-981-10-8252-8_12

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  • DOI: https://doi.org/10.1007/978-981-10-8252-8_12

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-8251-1

  • Online ISBN: 978-981-10-8252-8

  • eBook Packages: MedicineMedicine (R0)

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