To Estimate Creatinine Level in Serum and Urine by Jaffe’s Reaction
Creatinine is a waste product derived from dehydration of creatine and is excreted by the kidneys. It is synthesized in the kidneys, liver, and pancreas from three amino acids –arginine, glycine, and methionine – by two enzyme-mediated reactions. After synthesis, creatine is transported in the blood to other organs such as the muscle and brain and phosphorylated to phosphocreatine which is a high-energy compound. Some of the free creatine in the muscle is spontaneously converted to creatinine (anhydride of creatine). Creatine and creatine phosphate constitute about 400 mg/100 mg of fresh muscle. Both compounds are converted spontaneously to creatinine at rate of 1–2%.