To Determine the Blood Glucose Levels by Folin and Wu Method

  • Vijay Kumar
  • Kiran Dip Gill


The test is based on reducing property of glucose in hot alkaline solution. Proteins are precipitated by tungstic acid and removed by centrifugation. Protein-free filtrate of plasma contains glucose that reduces cupric ions of alkaline CuSO4 to cuprous oxide, which in turn reduces phosphomolybdic acid to molybdenum blue, and color thus produced is measured colorimetrically at 430 nm.

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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Vijay Kumar
    • 1
  • Kiran Dip Gill
    • 2
  1. 1.Department of BiochemistryMaharshi Dayanand UniversityRohtakIndia
  2. 2.Department of BiochemistryPostgraduate Institute of Medical Education & ResearchChandigarhIndia

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