In Vitro Morphogenesis of Some Himalayan Flora Using TDZ: A Potential Plant Growth Regulator

  • Nisha Dhiman
  • Nitu Gautam
  • Bhuvnesh Sareen
  • Pooja Kumari
  • Sapna Rajouria
  • Amita Bhattacharya
Chapter

Abstract

Thidiazuron or TDZ is a substituted phenylurea derivative, N-phenyl-N′-l, 2, 3-thidiazol-5-ylurea, extensively used in the in vitro morphogenesis of different plant species, including those found in Himalayas. The plants have ranged from the advanced monocotyledonous to dicotyledonous angiospermic plants to gymnosperms and even pteridophytes. While some of these plants are endemic to the Himalayas and are medicinally important, others have been introduced into the region for their immense commercial values. Optimal morphogenic responses obtained in these plants have varied with the concentration of TDZ used and its duration of exposure. These in turn have depended upon the explant and the media used, and also the plant species targeted. While in some cases TDZ alone evoked the desired responses, a combination of TDZ with other PGRs was required in other cases. The findings of these reports have been reviewed in the present article. Attempts have also been made to collate these findings into a ready reckoner for researchers working on in vitro morphogenesis of various plants inhabiting the Himalayas.

Keywords

Thidiazuron Continuous exposure Somatic embryogenesis Cytokinin-like activity Morphogenesis 

Abbreviations

2, 4-D

2, 4-Dichlorophenoxyacetic acid

B5

Gamborg medium

BAP

Benzylamino purine

DCR

Douglas-fir cotyledon revised medium

Kn

Kinetin

LS

Linsmaier and Skoog medium

MRM

Modified rhododendron medium

MS

Murashige and Skoog medium

NAA

Naphthalene acetic acid

nM

Nanomole

PGR

Plant growth regulator

PLBs

Protocorm-like bodies

pM

Picomole

TDZ

Thidiazuron

WPM

Woody plant medium

μM

Micromole

Notes

Acknowledgements

The authors thank Director, CSIR-IHBT, Palampur for providing the infrastructure. ND thanks the University Grants Commission, Govt. of India for providing Senior Research Fellowship. NG thanks the Department of Biotechnology, Govt. of India for providing her fellowship; BS thanks the Indian Council of Medical Research (ICMR), Govt. of India for providing Senior Research Fellowship. The CSIR-IHBT communication number for the present article is 4167.

References

  1. Aggarwal G, Sharma C, Srivastava DK (2012) Thidiazuron: a potent cytokinin for efficient plant regeneration in Himalayan poplar (Populus ciliata Wall.) using leaf explants. Ann For Res 55(2):147Google Scholar
  2. Ahmad N, Anis M (2007) Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agrofor Syst 71:195–200. https://doi.org/10.1007/s10457-007-9078-1 CrossRefGoogle Scholar
  3. Ahmed MR, Anis M (2012) Role of TDZ in the quick regeneration of multiple shoots from nodal explants of Vitex trifolia L. – an important medicinal plant. Appl Biochem Biotechnol 168:957–966PubMedCrossRefGoogle Scholar
  4. Ahmed N, Srivastava R, Anis M (2006) Improvement in carnation shoot multiplication using thidiazuron in vitro. Propag Ornam Plants 6:109–113Google Scholar
  5. Alatar AA (2015) Thidiazuron induced efficient in vitro multiplication and ex vitro conservation of Rauvolfia serpentina – a potent antihypertensive drug producing plant. Biotechnol Equip 29(3):489–497CrossRefGoogle Scholar
  6. Anis M, Husain MK, Faisal M, Shahzad A, Ahmad N, Siddique I, Khan H (2009) In vitro approaches for plant regeneration and conservation of some potential medicinal plants. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology & its applications, vol 14. I.K. International Pvt. Ltd., New Delhi, pp 397–410Google Scholar
  7. Arndt FR, Rusch R, Stillfried HV, Hanisch B, Martin WC (1976) A new cotton defoliant. Plant Physiol 57:S-99Google Scholar
  8. Bantawa J, Da Silva JAT, Ghosh SK, Mondal TK (2011) Determination of essential oil contents and micropropagation of Gaultheria fragrantissima, an endangered woody aromatic plant of India. J Hortic Sci Biotechnol 86(5):479–485CrossRefGoogle Scholar
  9. Bhat SJA, Gangoo SA, Geelani SM, Qasba SS, Parray AA (2014) Callus culture and organogenesis in fir (Abies pindrow Royle). J Cell Tissue Res 14(3):4653–4658Google Scholar
  10. Bhattacharyya P, Kumaria S, Diengdoh R, Tandon P (2014) Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid. Meta Gene 2:489–504PubMedPubMedCentralCrossRefGoogle Scholar
  11. Bhojwani SS, Dantu PK (2013) Plant tissue culture: an introductory text. Springer, London. http://dx.doi.org/10.1007/978-81-322-1026-9CrossRefGoogle Scholar
  12. Bisht S, Bisht NS, Bhandari S (2012) In vitro micropropagation in Polygonatum verticillatum (L.) All. An important threatened medicinal herb of Northern India. Physiol Mol Biol Plants 18(1):89–93. https://doi.org/10.1007/s12298-011-0091-5 PubMedCrossRefGoogle Scholar
  13. Brijwal L, Pandey A, Tamta S (2015) In vitro propagation of the endangered species Berberis aristata DC. via leaf-derived callus. In Vitro Cell Dev Biol Plant 51(6):637–647CrossRefGoogle Scholar
  14. Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Y2- isopentyl) [8-14c] adenosine in callus tissues of Phaseolus lunatus L. Plant Physiol 73:796–802PubMedPubMedCentralCrossRefGoogle Scholar
  15. Christianson ML, Warnick DA (1983) Competence and determination in the process of in vitro shoot organogenesis. Dev Biol 95(2):288–293PubMedCrossRefGoogle Scholar
  16. Datta MM, Majumder A, Jha S (2006) Organogenesis and plant regeneration in Taxus wallichiana (Zucc.) Plant Cell Rep 25:11–18. https://doi.org/10.1007/s00299-005-0027-z PubMedCrossRefGoogle Scholar
  17. Devi K, Sharma M, Ahuja PS (2014) Direct somatic embryogenesis with high frequency plantlet regeneration and successive cormlet production in saffron (Crocus sativus L.) S Afr J Bot 93:207–216CrossRefGoogle Scholar
  18. Faisal M, Anis M (2004) In vitro mass propagation and conservation of some endangered medicinal plants. In: D’Souza L, Anuradha M, Nivas S, Hegde S, Rajendra K (eds) Biotechnology for a better future. SAC Publications, Mumbai, pp 82–91Google Scholar
  19. Faisal M, Singh S, Anis M (2005) In vitro regeneration and plant establishment of Tylophora indica (Burm. f.) Merrill: petiole callus culture. In Vitro Cell Dev Biol Plant 41(4):511–515CrossRefGoogle Scholar
  20. Faisal M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Biol Plant 50(3):437–440CrossRefGoogle Scholar
  21. Gaba VC (2005) Plant growth regulators in tissue culture and development. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. CRC Press, Washington, DC, pp 87–99Google Scholar
  22. Gamborg OL, Miller RA, Ojima O (1968) Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  23. George EF et al (2008) Plant growth regulators I: introduction: auxins, their analogues and inhibitors. In: Plant propagation by tissue culture, 3rd edn. Springer, Netherlands, pp 175–204Google Scholar
  24. Gianfagna JT (1987) Natural and synthetic growth regulators and their use in horticultural and agronomic crops. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Martinus Nijhoff Publishers, Dordrecht, pp 614–635CrossRefGoogle Scholar
  25. Giri D, Tamta S (2011) Effect of plant growth regulators (PGRs) on micropropagation of a vulnerable and high value medicinal plant Hedychium spicatum. Afr J Biotechnol 10(20):4040–4045Google Scholar
  26. Gondval M, Chaturvedi P, Gaur AK (2016) Thidiazuron-induced high frequency establishment of callus cultures and plantlet regeneration in Aconitum balfourii Stapf.: an endangered medicinal herb of North-West Himalayas. Indian J Biotechnol 15:251–255Google Scholar
  27. Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10(45):8984–9000CrossRefGoogle Scholar
  28. Gupta PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4:177–179PubMedCrossRefGoogle Scholar
  29. Gupta S, Singh P, Ananda RA (2009) Micropropagation of mulberry (Morus indica L.) using explants from mature tree: effects of plant growth regulators on shoot multiplication and rooting. Progress Hortic 41:136–144Google Scholar
  30. Gurung B, Singh KK (2010) In vitro mass propagation of Sikkim Himalayan Rhododendron (R. dalhousiae Hook. f.) from nodal segment. J Am Soc Hortic Sci 12(1):42–45Google Scholar
  31. Haberlandt G (1902) Kulturversuche Mit Isolierten Pflanzenzellen. Sitzungsber Akad Wiss Wien Math-Naturwiss Kl Abt 111:69–92Google Scholar
  32. Hajong S, Kumaria S, Tandon P (2013) Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum Wall. ex Lindl. J Agric Sci Technol 15:1425–1435Google Scholar
  33. Hare PD, Staden JV (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35(8):1121–1125. https://doi.org/10.1093/oxfordjournals.pcp.a078704 CrossRefGoogle Scholar
  34. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33(20):105–119CrossRefGoogle Scholar
  35. Hutchinson MJ, Saxena PK (1996) Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in Geranium (Pelargonium x hortorum Bailey) tissue cultures. Plant Cell Rep 15(7):512–515PubMedCrossRefGoogle Scholar
  36. Jiang BO, Yang YG, Guo YM, Guo ZC, Chen YZ (2005) Thidiazuron-induced in vitro shoot organogenesis of the medicinal plant Arnebia euchroma (Royle) Johnst. In Vitro Cell Dev Biol Plant 41(5):677–681CrossRefGoogle Scholar
  37. Jime’nez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110CrossRefGoogle Scholar
  38. Johnson TS, Narayan SB, Narayana DBA (1997) Rapid in vitro propagation of Saussurea lappa, an endangered medicinal plant, through multiple shoot cultures. In Vitro Cell Dev Biol Plant 33(2):128–130CrossRefGoogle Scholar
  39. Kant R (2015) Survival threats and conservation of Malaxis muscifera (Lindl.) Kuntze, a threatened medicinal orchid at Fagu, Himachal Pradesh. Int J App Basic Med Res 1:2395–3373Google Scholar
  40. Kapruwan S, Bakshi M, Kaur M (2014) Effect of growth regulators on the in vitro multiplication of Dendrocalamus hamiltonii. Int J Eng Res Appl 1(4):83–86Google Scholar
  41. Kögl F, Haagen-Smit AJ, Erxleben H (1934) Über ein neues auxin (Heteroauxin) aus Harn. 11. mitteilung über pflanzliche wachstumsstoffe. Hoppe-Seyler’s Z Physiol Chem 228:90–103CrossRefGoogle Scholar
  42. Kou Y, Yuan C, Zhao Q, Liu G, Nie J, Ma Z, Cheng C, Silva JAT, Zhao L (2016) Thidiazuron triggers morphogenesis in Rosa canina L. protocorm-like bodies by changing incipient cell fate. Front Plant Sci. 04 7:557. https://doi.org/10.3389/fpls.2016.00557 PubMedPubMedCentralCrossRefGoogle Scholar
  43. Kumar A, Kaushal S, Sharma S, Sood H (2014) In vitro callus induction and plantlet regeneration of Swertia chirayita: a critically endangered medicinal plant. Int J Pharm Sci Rev Res 27(1):94–96Google Scholar
  44. Laimer M, Rüccker W (eds) (2003) Plant tissue culture 100 years since Gottlieb Haberlandt. Springer-Verlag Wien, New York, p 260Google Scholar
  45. Lee SW (2005) Thidiazuron in the improvement of banana micropropagation. Acta Hortic 692:67–74CrossRefGoogle Scholar
  46. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127CrossRefGoogle Scholar
  47. Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proc Int Plant Propagator’s Soc 30:421–427Google Scholar
  48. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol 29:92–96CrossRefGoogle Scholar
  49. Lu MC (2010) Plant regeneration from callus culture of an endangered orchid, Geodorum densiflorum. Int Orchid Sym 878:175–178Google Scholar
  50. Makara AM, Rubaihayo PR, Magambo MJS (2010) Carry-over effect of thidiazuron on banana in vitro proliferation at different culture cycles and light incubation conditions. Afr J Biotechnol 9(21):3079–3085Google Scholar
  51. Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L.: high frequency induction of direct shoot formation on intact seedlings by N6-benzylaminopurine and TDZ. Planta 186:384–389PubMedCrossRefGoogle Scholar
  52. Malik S, Sharma S, Sharma M, Ahuja PS (2010) Direct shoot regeneration from intact leaves of Arnebia euchroma (Royle) Johnston using thidiazuron. Cell Biol Int 34:537–542PubMedCrossRefGoogle Scholar
  53. Mathur G, Nadgauda R (1999) In vitro plantlet regeneration from mature zygotic embryos of Pinus wallichiana A.B. Jacks. Plant Cell Rep 19:74–80CrossRefGoogle Scholar
  54. Mehta R (2012) Development of efficient regeneration systems in economically important edible bamboos. Ph.D thesis. Guru Nanak Dev University, AmritsarGoogle Scholar
  55. Miller CO, Skoog F, von Saltza MH, Strong M (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J Am Chem Soc 77:1329–1334Google Scholar
  56. Mitra GC, Prasad RN, Roychowdhury A (1976) Inorganic salts and differentiation of protocorms in seed callus of orchid and correlative changes in its free amino acid content. Indian J Exp Biol 14:350–351Google Scholar
  57. Mok MC, Mok DWS (1985) The metabolism of [14C]-tMdiaziiroii in callus tissues of Phaseolus lunatus. Physiol Plant 65:427–432. https://doi.org/10.1111/j.1399-3054.1985.tb08668.x CrossRefGoogle Scholar
  58. Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-N′-1,2,3-thidiazol-5-yl urea (thidiazuron). Phytochemistry 21:1509–1511CrossRefGoogle Scholar
  59. Mok MC, Mok DWS, Turner JE, Mujer CV (1987) Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. Hortic Sci 22:1194–1197Google Scholar
  60. Mokhtari A, Otroshy M, Barekat T (2015) Plant regeneration through callus induction on medicinal herb Viola odorata – role of plant growth regulators and explants. Agric For 61(3):161–170. https://doi.org/10.17707/AgricultForest.61.3.16 Google Scholar
  61. Mondal TK, Bhattacharya A, Sood A, Ahuja PS (1998) Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using thidiazuron. Plant Growth Regul 26:57–61CrossRefGoogle Scholar
  62. Murashige T. (1979). Plant growth substances in commercial uses of tissue culture. Plant Growth Subst 426–434. https://doi.org/10.1007/978-3-642-67720-5_43
  63. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  64. Murthy BNS, Victor J, Singh RP, Fletcher RA, Saxena PK (1996) In vitro regeneration of chickpea (Cicer arietinum L.): stimulation of direct organogenesis and somatic embryogenesis by thidiazuron. Plant Growth Regul 19:233–240CrossRefGoogle Scholar
  65. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267. https://doi.org/10.1007/BF02822732 CrossRefGoogle Scholar
  66. Murthy K, Reddy MC, Kondamudi R, Pullaiah T (2013) Micropropagation of Stemona tuberosa Lour. – an endangered and rare medicinal plant in Eastern Ghats of India. Indian J Biotechnol 12:420–424Google Scholar
  67. Nayak NR, Rath SP, Patnaik S (1997) In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci Hortic 71(3–4):243–250CrossRefGoogle Scholar
  68. Nickell LG (1994) Plant growth regulators in agriculture and horticulture. In: Hedin P (ed) Bioregulators for crop protection and pest control, ACS symposium series, vol 557, pp 1–14. https://doi.org/10.1021/bk-1994-0557.ch001 CrossRefGoogle Scholar
  69. Nielsen JM, Kirsten B, Hansen J (1993) Long-term effects of thidiazuron are intermediate between Benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis. Plant Cell Tissue Organ Cult 35:173–179CrossRefGoogle Scholar
  70. Ochatt SJ, Atif RM, Patat-Ochatt EM, Jacas L, Conreux C (2010) Competence versus recalcitrance for in vitro regeneration. Not Bot Hort Agrobot Cluj 38:102–108Google Scholar
  71. Panda BM, Mehta UJ, Hazra S (2016) Micropropagation of Semecarpus anacardium L. from mature tree-derived nodal explants. Plant Biosyst 150(5):942–952CrossRefGoogle Scholar
  72. Patial V, Devi K, Sharma M, Bhattacharya A, Ahuja PS (2012) Propagation of Picrorhiza kurroa Royle ex Benth: an important medicinal plant of Western Himalaya. J Med Plant Res 6(34):4848–4860Google Scholar
  73. Patial V, Sharma M, Bhattacharya A (2017) Potential of thidiazuron in improved micropropagation of Picrorhiza kurroa – an endangered medicinal herb of alpine Himalaya. Plant Biosyst 151(4):729–736CrossRefGoogle Scholar
  74. Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003) High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Rep 21:1054–1059PubMedCrossRefGoogle Scholar
  75. Preece JE, Huetteman CA, Ashaby WC, Roth PL (1991) Micro- and cutting propagation of silver maple I Results with adult and juvenile propagules. J Am Soc Hortic Sci 116:142–148Google Scholar
  76. Pulianmackal AJ, Kareem AVK, Durgaprasad K, Trivedi ZB, Prasad K (2014) Competence and regulatory interactions during regeneration in plants. Front Plant Sci 5:142. https://doi.org/10.3389/fpls.2014.00142 PubMedPubMedCentralCrossRefGoogle Scholar
  77. Quadri RR, Da Silva JAT, Kamili AN, Shah AM (2011) Effect of 6-benzyladenine, kinetin and thidiazuron on in vitro shoot proliferation of Hyoscyamus niger L. Med Aromat Plant Sci Biotechnol 6(1):81–83Google Scholar
  78. Rafi S, Kamili AZ, Ganai BA, Mir MY, Parray JA (2016) In vitro culture and biochemical attributes of Bergenia ciliata (Haw.) Sternb. Proc Natl Acad Sci India, Sect B Biol Sci. https://doi.org/10.1007/s40011-016-0797-9
  79. Rajender G, Bheemanna D, Sreenu P, Prasada B, Rajender K, Reuben T, Christopher (2017) In vitro clonal propagation of vulnerable ethnomedicinal cucurbit, red ball snake gourd (Trichosanthes tricuspidata lour.) Int J Pharm Bio Sci 8(2):205–209Google Scholar
  80. Ranyaphia RA, Maoa AA, Borthakurb SK (2011) Direct organogenesis from leaf and internode explants of in vitro raised wintergreen plant (Gaultheria fragrantissima). Sci Asia 37:186–194CrossRefGoogle Scholar
  81. Roberts JA, Hooley R (1988) Cellular differentiation and morphogenesis. In: Plant growth regulators. Tertiary level biology. Springer, BostonGoogle Scholar
  82. Roh KH, Kwak BK, Kim JB, Lee KR, Kim HU, Kim SH (2012) The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus Kyung. J Plant Biotechnol 39:133–139CrossRefGoogle Scholar
  83. Roy AR, Sajeev S, Pattanayak A, Deka BC (2012) TDZ induced micropropagation in Cymbidium giganteum Wall. Ex Lindl. and assessment of genetic variation in the regenerated plants. Plant Growth Regul 68(3):435–445CrossRefGoogle Scholar
  84. Sakhanokho HF, Kelly RW, Rajashekaran K (2008) First report of plant regeneration via somatic embryogenesis from shoot apex-derived callus of Hedychium muluense. J Crop Imp 21(2):191–200. https://doi.org/10.1080/15427520701885758 CrossRefGoogle Scholar
  85. Sandal I, Bhattacharya A, Ahuja PS (2001) An efficient liquid culture system for tea shoot proliferation. Plant Cell Tissue Organ Cult 65:75–80CrossRefGoogle Scholar
  86. Sang YL, Cheng ZJ, Zhang XS (2016) Endogenous auxin biosynthesis and de novo root organogenesis. J Exp Bot 67(14):4011–4013. https://doi.org/10.1093/jxb/erw250 PubMedPubMedCentralCrossRefGoogle Scholar
  87. Sharma VK, Hansch R, Mendel RR, Schulze J (2007) Node-derived cultures with high-morphogenic competence in barley and wheat. Plant Cell Tissue Organ Cult 88:21–33. https://doi.org/10.1007/s11240-006-9172-5 CrossRefGoogle Scholar
  88. Sharma E, Karma T, Chettri N, Shrestha A (2009) Biodiversity in the Himalayas – trends, perception and impacts of climate change. IMBC-Plenary session 1: climate change and its implications for mountainGoogle Scholar
  89. Sharma S, Katoch V, Rathour R, Sharma TR (2010) In vitro propagation of endangered temperate Himalayan medicinal herb Picrorhiza kurroa Royle ex benth using leaf explants and nodal segments. J Appl Biochem Biotechnol 19(1):111–114Google Scholar
  90. Sharma A, Kaur R, Sharma N (2014) In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant. Physiol Mol Biol Plants 20(2):249–256PubMedPubMedCentralCrossRefGoogle Scholar
  91. Siddique I, Anis M (2007) Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium. Biol Plant 51:787–790CrossRefGoogle Scholar
  92. Singh DK, Babbar SB (2016) In vitro propagation and chemical profiling of Herminium lanceum (Thunb. ex Sw.) Vuijk, a medicinally important orchid, for therapeutically important phenolic acids. Plant Biotechnol J 33(3):153–160CrossRefGoogle Scholar
  93. Singh SK, Syamal MM (2001) A short pre-culture soak in thidiazuron or forchlorfenuron improves axillary shoot proliferation in rose micropropagation. Sci Hortic 91:169–177CrossRefGoogle Scholar
  94. Singh S, Purohit VK, Prasad P, Nautiyal AR (2015) Micropropagation of Valeriana wallichii DC. (Indian Valerian) through nodes. Indian J Biotechnol 14:127–130Google Scholar
  95. Tabin S, Gupta RC, Kamili AN (2014) In vitro micro propagation of Rheum explants supplemented with various types of growth hormones. J Agric Vet Sci 7(3):97–100Google Scholar
  96. Taha RM, Haron NW, Wafa SN (2011) Morphological and tissue culture studies of Platycerium coronarium, a rare ornamental Fern species from Malaysia. Am Fern J 101(4):241–251CrossRefGoogle Scholar
  97. Tang W, Newton RJ (2005) Peroxidase and catalase activities are involved in direct adventitious shoot formation induced by thidiazuron in Eastern white pine (Pinus strobus L.) zygotic embryos. Plant Physiol Biochem 43(8):760–769PubMedCrossRefGoogle Scholar
  98. Tao J, Yu L, Kong F, Zhao D (2011) Effect of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe. Afr J Biotechnol 10:15639–15646Google Scholar
  99. Thakur RC, Hosoi Y, Ishii K (1998) Rapid in vitro propagation of Matteuccia struthiopteris (L.)Todaro – an edible fern. Plant Cell Rep 18:203–208CrossRefGoogle Scholar
  100. Thomas JC, Katterman FR (1986) Cytokinin activity induced by thidiazuron. Plant Physiol 81(2):681–683PubMedPubMedCentralCrossRefGoogle Scholar
  101. Vengadesan G, Pijut PM (2009) In vitro propagation of northern red oak (Quercus rubra L.) In Vitro Cell Dev Biol-Plant 45:474–482. https://doi.org/10.1007/s11627-008-9182-6 CrossRefGoogle Scholar
  102. Verma M, Bansal YK (2014) Effect of a potent cytokinin thidiazuron (TDZ) on in vitro regeneration of Hedychium coronarium J. Koenig – a valuable medicinal plant. Int J Rec Biotech 2(1):38–44Google Scholar
  103. Vijayakumar M, Vijayakumar R, Stephen R (2010) In vitro propagation of Bacopa monnieri L. – a multipurpose medicinal plant. Indian J Sci Technol 3(7):782–787Google Scholar
  104. Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of thidiazuron’ substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol 99:1704–1707PubMedPubMedCentralCrossRefGoogle Scholar
  105. Winarto B, da Silva JT (2012) Improved micropropagation protocol for leatherleaf fern (Rumohra adiantiformis) using rhizomes as donor explants. Sci Hortic 140:74–80CrossRefGoogle Scholar
  106. Yu R, Zhang G, Li H, Cao H, Mo X, Gui M, Zhou X, Jiang Y, Li S, Wang J (2017) In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies. Plant Cell Tissue Organ Cult 128:369–379CrossRefGoogle Scholar
  107. Zhao D, Hu G, Chen Z, Shi Y, Zheng L, Tang A, Long C (2013) Micropropagation and in vitro flowering of Dendrobium wangliangii: a critically endangered medicinal orchid. J Med Plant Res 7(28):2098–2110CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Nisha Dhiman
    • 1
    • 2
  • Nitu Gautam
    • 2
  • Bhuvnesh Sareen
    • 2
  • Pooja Kumari
    • 2
  • Sapna Rajouria
    • 2
  • Amita Bhattacharya
    • 1
    • 2
  1. 1.Academy of Scientific and Innovative ResearchNew DelhiIndia
  2. 2.Division of BiotechnologyCSIR-Institute of Himalayan Bioresource TechnologyPalampurIndia

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