Colorimetric Sandwich Assays for Protein Detection

Chapter

Abstract

For decades, the sandwich assays have been widely used in quality control, clinical diagnostics, biological detection, and environmental monitoring field. Apart from the requirement of labeling the molecular target, the sandwich assay generally requires the recognition and signaling probe to be combined, which gives them accurate specific. Also, the optical sensing method is of great interest due to the intrinsic high sensitivity and simplicity. The colorimetric assays are much simpler for the detection of analytes, and their response can be directly detected with bare eyes or by photometry compared with other analytical techniques. Colorimetric sandwich assays are usually based on the observable color variation in the presence of enzyme-labeled antibody. In this chapter, we focus on the detection of protein by colorimetric sandwich assays based on traditional enzymes and biomimetic nanomaterials. The detection technologies employed in colorimetric sandwich assays based on traditional enzymes were alkaline phosphatase (ALP), horseradish peroxidase (HRP), open sandwich immunoassay (OS-IA), and gold–multienzyme–nanocarrier. For the biomimetic nanomaterials, highlighted examples were focused on Fe3O4 magnetic nanoparticles (MNPs) biomimetic enzymes, and Au@Pt nanostructure biomimetic enzymes.

Keywords

Colorimetric sandwich assays Protein detection Biomimetic enzymes Traditional enzymes Nanoparticles 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and ChemistryChina University of GeosciencesWuhanPeople’s Republic of China
  2. 2.Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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