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Inflammatory Response of Bioscaffolds Decellularized by Sonication Treatment

  • Conference paper
  • First Online:
2nd International Conference for Innovation in Biomedical Engineering and Life Sciences (ICIBEL 2017)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 67))

Abstract

Decellularization of sonication system efficiency is dictated by evaluation of host immune response on scaffolds. This aim of this study is to examine the inflammatory response after implantation of bioscaffolds decellularized by sonication treatment at 35 days post-surgery in rats. In this study, aortic tissues decellularized by sonication treatment in 0.1 and 2% of Sodium Dodecyl Sulfate (SDS) detergents. Samples were washed for 5 days in Phosphate Buffer Saline (PBS) following decellularization. Samples were implanted in on the lower right thoracic cavity of rats for 35 days. To examine the inflammatory response, the implanted samples were explanted and evaluated histologically by Hematoxylin Eosin (H-E) staining. From the histological analysis, bioscaffolds decellularized by sonication treatment in response to macrophages shows the minimal inflammatory response. Minimal inflammatory response elicits by bioscaffolds suggests the safety of bioscaffolds prepared by sonication treatment as biomedical implants.

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References

  1. Sheikh, Z., Brooks, P.J., Barzilay, O., Fine, N., Glogauer, M.: Macrophages, foreign body giant cells and their response to implantable biomaterials. Materials 8(9), 5671–5701 (2015)

    Article  Google Scholar 

  2. Syazwani, N., Azhim, A., Morimoto, Y., Furukawa, K.S., Ushida, T.: Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering. J. Med. Biol. Eng. 35(2), 258–269 (2015)

    Article  Google Scholar 

  3. Smith, M.J., Smith, D.C., White, K.L., Bowlin, G.L.: Immune response testing of electrospun polymers: an important consideration in the evaluation of biomaterials. J. Eng. Fibers Fabr. 2(2), 41–47 (2007)

    Google Scholar 

  4. Crapo, P.M., Gilbert, T.W., Badylak, S.F.: An overview of tissue and whole organ decellularization processes. Biomaterials 32(12), 3233–3243 (2011)

    Article  Google Scholar 

  5. Koch, H., Graneist, C., Emmrich, F., Till, H., Metzger, R., Aupperle, H., Schierle, K., Sack, U., & Boldt, A.: Xenogenic esophagus scaffolds fixed with several agents : Comparative in vivo study of rejection an inflammation. J. Biomed. Biotechnol. 2012, 1–11 (2012)

    Google Scholar 

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Acknowledgements

The authors are grateful to the Ministry of Higher Education for financial support through Fundamental Research Grant Scheme (FRGS15-204-0445), Prototype Research Grant Scheme (PRGS16-002-0033) and Transdiciplinary Research Grant Scheme (TRGS16-02-001-0001).

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Authors and Affiliations

  1. Department of Biotechnology, Kulliyyah of Sciences, IIUM, Kuantan Campus, Malaysia

    Aqilah Hazwani & Azran Azhim

  2. Kulliyyah of Allied Health Sciences, IIUM, Kuantan Campus, Malaysia

    Aqilah Hazwani, Munirah Sha’ban & Azran Azhim

Authors
  1. Aqilah Hazwani
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  2. Munirah Sha’ban
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  3. Azran Azhim
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Corresponding author

Correspondence to Azran Azhim .

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Editors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Fatimah Ibrahim

  2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Juliana Usman

  3. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Mohd Yazed Ahmad

  4. Department of Rehabilitation Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    Norhamizan Hamzah

  5. Department of Biomedical Engineering, Faculty of Engineering, Centre for Innovation in Medical Engineering, University of Malaya, Kuala Lumpur, Malaysia

    Swe Jyan Teh

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© 2018 Springer Science+Business Media Singapore

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Hazwani, A., Sha’ban, M., Azhim, A. (2018). Inflammatory Response of Bioscaffolds Decellularized by Sonication Treatment. In: Ibrahim, F., Usman, J., Ahmad, M., Hamzah, N., Teh, S. (eds) 2nd International Conference for Innovation in Biomedical Engineering and Life Sciences. ICIBEL 2017. IFMBE Proceedings, vol 67. Springer, Singapore. https://doi.org/10.1007/978-981-10-7554-4_32

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  • DOI: https://doi.org/10.1007/978-981-10-7554-4_32

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-7553-7

  • Online ISBN: 978-981-10-7554-4

  • eBook Packages: EngineeringEngineering (R0)

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