Transgenic Techniques for Investigating Cell Biology During Development
Ascidians are increasingly being used as a system for investigating cell biology during development. The extreme genetic and cellular simplicity of ascidian embryos in combination with superior experimental tractability make this an ideal system for in vivo analysis of dynamic cellular processes. Transgenic approaches to cellular and sub-cellular analysis of ascidian development have begun to yield new insights into the mechanisms regulating developmental signaling and morphogenesis. This chapter focuses on the targeted expression of fusion proteins in ascidian embryos and how this technique is being deployed to garner new insights into the cell biology of development.
KeywordsAscidians Ciona intestinalis Phallusia mammillata Halocynthia roretzi Cardiac induction Collective cell migration Cell cycle progression Spindle positioning Fusion proteins
The author would like to thank Dr. Brad Davidson (Swarthmore College) for comments on the manuscript. Work in the Davidson laboratory is supported by Swarthmore College and the NIH (1R01HL091027, R15 HD080525-01). CDC is supported by an American Heart Association Postdoctoral Award (16POST27250075).
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