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TALEN-Based Knockout System

  • Keita Yoshida
  • Nicholas Treen
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1029)

Abstract

Targeted mutagenesis of genes-of-interest is a powerful method of addressing the functions of genes. Genome editing techniques, such as transcriptional activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 systems, have enabled this approach in various organisms because of their ease of use. In the ascidian, Ciona intestinalis, recent studies show that TALEN-based knockout can be applied to establishing both mutant lines and tissue-specific knockout for addressing gene functions. Here, we introduce recent updates to the TALEN toolkit that facilitate detailed functional analysis of genes in ascidians.

Keywords

Ascidian Ciona intestinalis Genome editing TALEN Conditional knockout 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Shimoda Marine Research CenterUniversity of TsukubaShimodaJapan
  2. 2.Lewis-Sigler Institute for Integrative GenomicsPrinceton UniversityPrincetonUSA

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