Abstract
A number of protein-protein complexes are associated with disease progression and are considered as targets for drug and vaccine development. Therefore, it is important to understand protein-protein interfaces in these disease-linked complexes. We illustrate and discuss this phenomenon using a hexameric (six subunits) cholera toxin (CT) and a HIV-1/ENV GP160 (GP120/GP40) trimer (three subunits) spike protein complex. Analysis of subunit-subunit interfaces in these complexes play an important role in the design, development, and production of a stable yet a viable vaccine.
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References
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Exercises
Exercises
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1.
Illustrate the structure of a functional cholera toxin.
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2.
How many subunits constitute a cholera toxin?
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3.
Illustrate the HIV-1 spike protein using a neat diagram.
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4.
How many subunits constitute HIV-1/ENV GP160 trimer?
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5.
Discuss the issues in the development of an effective cholera vaccine.
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6.
Discuss the issues in the development of an effective HIV-1/AIDS vaccine.
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7.
Illustrate the CTB and CTA interface in a cholera toxin.
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8.
Illustrate the CTB interfaces in a cholera toxin.
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9.
Illustrate the GP120/GP40 interface in the HIV-1/ENV protein.
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10.
Discuss HIV-1/ENV GP160 trimer spike structure.
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Kangueane, P., Nilofer, C. (2018). Protein-Protein Interfaces and Diseases. In: Protein-Protein and Domain-Domain Interactions. Springer, Singapore. https://doi.org/10.1007/978-981-10-7347-2_16
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DOI: https://doi.org/10.1007/978-981-10-7347-2_16
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Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-7346-5
Online ISBN: 978-981-10-7347-2
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