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Regulation of Signal Transduction in Human Cell Research pp 1–27Cite as

Using Genetically Engineered Mouse Models to Study Wnt Signaling in Bone Development and Disease

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Part of the Current Human Cell Research and Applications book series (CHCRA)

Abstract

The skeleton supports the body structure and reserves calcium and other inorganic ions, and more roles played by bone are being proposed. The balance between bone formation (by osteoblasts and osteocytes) and bone resorption (by osteoclasts) controls postnatal bone homeostasis. For the past decade, a vast amount of evidence has shown that Wnt signaling plays a pivotal role in regulating this balance. Therefore, understanding how the Wnt signaling pathway regulates skeletal development and postnatal homeostasis is of great value for human skeletal health. We will review how genetically engineered mouse models (GEMMs) have been and are being used to uncover the mechanisms and etiology of bone diseases in the context of Wnt signaling.

Keywords

  • Wnt signaling
  • Bone development
  • Transgenic mice
  • Conditional knock out
  • Cre-loxP
  • Tissue-specific promoter

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Fig. 1.1
Fig. 1.2
Fig. 1.3
Fig. 1.4
Fig. 1.5
Fig. 1.6

Abbreviations

CKO:

Conditional knockout

Fzd:

Frizzled

GEMMs:

Genetically engineered mouse models

GOF:

Gain of function

KO:

Full-body knockout

Lrp:

Low-density lipoprotein-related receptor protein

LBM:

Low bone mass

LEF:

Lymphoid enhancer factor

LOF:

Loss of function

MSC:

Mesenchymal stem cell

M-CSF:

Macrophage colony-stimulating factor

NA:

Not applicable

OE:

Overexpression

OMIM:

Online Mendelian Inheritance in Man catalog

OPG:

Osteoprotegerin

RANKL:

Receptor activator of nuclear factor kappa-B ligand

TCF:

T-cell factor

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Authors and Affiliations

  1. Program in Skeletal Disease and Tumor Microenvironment, Center for Cancer and Cell Biology, Van Andel Research Institute, Grand Rapids, MI, USA

    Zhendong A. Zhong, Nicole J. Ethen & Bart O. Williams

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  1. Zhendong A. Zhong
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  2. Nicole J. Ethen
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  3. Bart O. Williams
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Correspondence to Bart O. Williams .

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Editors and Affiliations

  1. Department of Integrative Physiology and Bio-Nano Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan

    Dr. Nariyoshi Shinomiya

  2. Department of Pathology, University of Miyazaki, Miyazaki, Miyazaki, Japan

    Dr. Hiroaki Kataoka

  3. Department of Biomedical Sciences, Center of Excellence for Inflammation, Infectious Disease and Immunity, Quillen College of Medicine, East Tennessee State University, Johnson, Tennessee, USA

    Dr. Qian Xie

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Zhong, Z.A., Ethen, N.J., Williams, B.O. (2018). Using Genetically Engineered Mouse Models to Study Wnt Signaling in Bone Development and Disease. In: Shinomiya, N., Kataoka, H., Xie, Q. (eds) Regulation of Signal Transduction in Human Cell Research. Current Human Cell Research and Applications. Springer, Singapore. https://doi.org/10.1007/978-981-10-7296-3_1

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