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Using Genetically Engineered Mouse Models to Study Wnt Signaling in Bone Development and Disease

  • Zhendong A. Zhong
  • Nicole J. Ethen
  • Bart O. Williams
Chapter
Part of the Current Human Cell Research and Applications book series (CHCRA)

Abstract

The skeleton supports the body structure and reserves calcium and other inorganic ions, and more roles played by bone are being proposed. The balance between bone formation (by osteoblasts and osteocytes) and bone resorption (by osteoclasts) controls postnatal bone homeostasis. For the past decade, a vast amount of evidence has shown that Wnt signaling plays a pivotal role in regulating this balance. Therefore, understanding how the Wnt signaling pathway regulates skeletal development and postnatal homeostasis is of great value for human skeletal health. We will review how genetically engineered mouse models (GEMMs) have been and are being used to uncover the mechanisms and etiology of bone diseases in the context of Wnt signaling.

Keywords

Wnt signaling Bone development Transgenic mice Conditional knock out Cre-loxP Tissue-specific promoter 

Abbreviations

CKO

Conditional knockout

Fzd

Frizzled

GEMMs

Genetically engineered mouse models

GOF

Gain of function

KO

Full-body knockout

Lrp

Low-density lipoprotein-related receptor protein

LBM

Low bone mass

LEF

Lymphoid enhancer factor

LOF

Loss of function

MSC

Mesenchymal stem cell

M-CSF

Macrophage colony-stimulating factor

NA

Not applicable

OE

Overexpression

OMIM

Online Mendelian Inheritance in Man catalog

OPG

Osteoprotegerin

RANKL

Receptor activator of nuclear factor kappa-B ligand

TCF

T-cell factor

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Zhendong A. Zhong
    • 1
  • Nicole J. Ethen
    • 1
  • Bart O. Williams
    • 1
  1. 1.Program in Skeletal Disease and Tumor Microenvironment, Center for Cancer and Cell BiologyVan Andel Research InstituteGrand RapidsUSA

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