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How to Analyze and Present SAS Data for Publication

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Biological Small Angle Scattering: Techniques, Strategies and Tips

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1009))

Abstract

SAS is a powerful technique to investigate oligomeric state and domain organization of macromolecules, e.g. proteins and nucleic acids, under physiological, functional and even time resolved conditions. However, reconstructing three dimensional structures from SAS data is inherently ambiguous, as no information about orientation and phase is available. In addition experimental artifacts such as radiation damage, concentration effects and incorrect background subtraction can hinder the interpretation of even lead to wrong results. In this chapter, explanations on how to analyze data and how to assess and minimize the influence of experimental artifacts on the data. Furthermore, guidelines on how to present the resulting data and models to demonstrate the data supports the conclusion being made and that it is not biased by artifacts, will be given.

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Acknowledgments

The authors would like to thank Dr. Bárbara Calisto Machado for providing the SAXS data of the K and G proteins and KG complex.

Author information

Authors and Affiliations

  1. European Molecular Biology Laboratory, Grenoble, 71 avenue des Martyrs, 38042, Grenoble, France

    Martha Brennich

  2. Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, 71 avenue des Martyrs, 38042, Grenoble, France

    Petra Pernot

  3. SPB/SFX instrument, European XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany

    Adam Round

  4. Faculty of Natural Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK

    Adam Round

Authors
  1. Martha Brennich
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  2. Petra Pernot
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  3. Adam Round
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Corresponding author

Correspondence to Martha Brennich .

Editor information

Editors and Affiliations

  1. G N Ramachandran Protein Center, CSIR Institute of Microbial Technology, Chandigarh, India

    Barnali Chaudhuri

  2. Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain

    Inés G. Muñoz

  3. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Shuo Qian

  4. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Volker S. Urban

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Brennich, M., Pernot, P., Round, A. (2017). How to Analyze and Present SAS Data for Publication. In: Chaudhuri, B., Muñoz, I., Qian, S., Urban, V. (eds) Biological Small Angle Scattering: Techniques, Strategies and Tips. Advances in Experimental Medicine and Biology, vol 1009. Springer, Singapore. https://doi.org/10.1007/978-981-10-6038-0_4

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