Potential of Fungal Endophytes in Plant Growth and Disease Management

  • Kanika Chowdhary
  • Satyawati SharmaEmail author


The historical background, various investigations, successful results and projected mode of activity for overall plant development and disease management conferred by fungal endophytes to their host plants have been elaboratively discussed. Piriformospora indica, a root endophyte, is the well-described plant promoter in literature. 2H-Pyran-2-one, 5,6-dihydro-6-pentyl, an antifungal metabolite with high activity towards S. sclerotiorum, was produced by endophytic Macrophomina phaseolina, while solanapyrone derivatives C and phomalactone produced by Nigrospora sp. YB-141 showed inhibition against plant pathogenic fungus Botrytis cinerea. The present chapter summarises various findings on fungal endophytes and their functional attributes with respect to enhancement in overall plant maturity and improvement recovered from indigenous plant of medicinal value Ocimum sanctum found widely in India till date.


Endophytic fungi Ocimum sanctum Biopesticides Indigenous plants Antifungal activity 



Kanika Chowdhary would like to acknowledge the financial assistance provided by DST-NPDF scheme (grant no. PDF/2016/000317) and CRDT, IIT Delhi, for providing infrastructural support.


  1. Abubacker MN, Devi PK (2014) In vitro antifungal potentials of bioactive compound oleic acid, 3-(octadecyloxy) propylester isolated from Lepidagathis cristata Willd. (Acanthaceae) inflorescence. Asian Pac J Trop Biomed 4:S661–S664CrossRefGoogle Scholar
  2. Aly AH, Debbab A, Proksch P (2011) Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol 90:1829–1845CrossRefPubMedGoogle Scholar
  3. Amin N (2015) Nematicidal activity of root exudates of sengon plant inoculated with endophytic fungi Nigrospora sp. to control of root-knot nematode Meloidogyne spp. J Chem Pharm Res 7(3):307–310Google Scholar
  4. Andrade-Linares DR, Müller A, Fakhro A, Schwarz D, Franken P (2013) Impact of Piriformospora indica on tomato. Soil Biol 33:107–117CrossRefGoogle Scholar
  5. Arnold AE, Mejía LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA (2003) Fungal endophytes limit pathogen damage in a tropical tree. PNAS USA 100:15649–15654CrossRefPubMedPubMedCentralGoogle Scholar
  6. Baltruschat H, Fodor J, Harrach BD, Niemczyk E, Barna B, Gullner G, Janeczko A, Kogel KH, Schäfer P, Schwarczinger I, Zuccaro A, Skoczowski A (2008) Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Phytol 180:501–510CrossRefPubMedGoogle Scholar
  7. Banerjee D, Manna S, Mahapatra S, Pati BR (2009) Fungal endophytes in three medicinal plants of Lamiaceae. Acta Microbiol Immunol Hung 56(3):243–250. doi: 10.1556/AMicr.56.2009.3.4 CrossRefPubMedGoogle Scholar
  8. Bhagat J, Kaur A, Sharma M, Saxena AK, Chadha BS (2012) Molecular and functional characterization of endophytic fungi from traditional medicinal plants. World J Microbiol Biotechnol 28(3):963–971. doi: 10.1007/s11274-011-0894-0 CrossRefPubMedGoogle Scholar
  9. Bing LA, Lewis LC (1991) Suppression of Ostrinia nubilalis (Hübner) (Lepidoptera: Pyralidae) by endophytic Beauveria bassiana (Balsamo) Vuillemin. Environ Entomol 20:1207–1211CrossRefGoogle Scholar
  10. Brown KB, Hyde KD, Guest DJ (1998) Preliminary studies on endophytic fungal communities of Musa acuminata species complex in Hong Kong and Australia. Fungal Divers 1:27–51Google Scholar
  11. Carroll GE (1986) The biology of the endophytism in plants with particular reference to woody perennials. In: Fokkema NJ, van den Heuvel J (eds) The microbiology of the phyllosphere. Cambridge University Press, Cambridge, pp 205–222Google Scholar
  12. Chen Y, Peng Y, Dai CC, Ju Q (2011) Biodegradation of 4-hydroxybenzoic acid by Phomopsis liquidambari. Appl Soil Ecol 51:102–110CrossRefGoogle Scholar
  13. Chen YM, Yang YH, Li XN, Zou C, Zhao PJ (2015) Diterpenoids from the endophytic fungus Botryosphaeria sp. P483 of the Chinese herbal medicine Huperzia serrata. Molecules 20(9):16924–16932. doi: 10.3390/molecules200916924 CrossRefPubMedGoogle Scholar
  14. Cherry AJ, Banito A, Djegui D, Lomer C (2004) Suppression of the stem borer Sesamia calamistis (Lepidoptera: Noctuidae) in maize following seed dressing, topical application and stem injection with African isolates of Beauveria bassiana. Agric Entomol 7:171–181Google Scholar
  15. Chowdhary K, Kaushik N (2015) Fungal endophyte diversity and bioactivity in the Indian medicinal plant Ocimum sanctum Linn. PLoS One 10(11):e0141444. doi: 10.1371/journal.pone.014144 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Chowdhary K, Kaushik N, Coloma AG, Raimundo CM (2012) Endophytic fungi and their metabolites isolated from Indian medicinal plant. Phytochem Rev 11:467–485. doi: 10.1007/s11101-012-9264 CrossRefGoogle Scholar
  17. Coombs JT, Franco CMM (2003) Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69:5603–5608. CrossRefPubMedPubMedCentralGoogle Scholar
  18. Firakova S, Sturdikova M, Muckova M (2007) Bioactive secondary metabolites produced by microorganisms associated with plants. Biology 62:251–257Google Scholar
  19. Freeman EM (1904) The seed fungus of Lolium temulentum L. Darnel. Phil Trans R Soc Land B 196:1–2CrossRefGoogle Scholar
  20. Gao FK, Dai CC, Liu XZ (2010) Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res 4(13):1346–1351Google Scholar
  21. Gautam A (2014) Diversity of fungal endophytes in some medicinal plants of Himachal Pradesh, India. Archives Phytopathol Plant Prot 47(5). doi: 10.1080/03235408.2013.813678
  22. Hardoim PR, Van Overbeek LS, Berg G, Pirttilä AM, Compant S, Campisano A, Döring M, Sessitsch A (2015) The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiol Mol Biol Rev. doi: 10.1128/MMBR.00050-14
  23. Harper JK, Arif AM, Ford EJ, Strobel GA Jr, Porco JA, Tomer DP, Oneill KL, Heider EM, Grant DM (2003) Pestacin: a 1,3-dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities. Tetrahedron 59:2471–2476CrossRefGoogle Scholar
  24. Hu Y, Zhang W, Zhang P, Ruan W, Zhu X (2013) Nematicidal activity of chaetoglobosin a produced by Chaetomium globosum NK102 against Meloidogyne incognita. J Agric Food Chem 61:41–46CrossRefPubMedGoogle Scholar
  25. Huang WY, Cai YZ, Hyde KD, Corke H, Sun M (2007) Endophytic fungi from Nerium oleander L (Apocynaceae):main constituents and antioxidant activity. World J Microbiol Biotechnol 23:1253–1263CrossRefGoogle Scholar
  26. Hussain H, Krohn K, Draeger S, Meier K, Schulz B (2009) Bioactive chemical constituents of a sterile endophytic fungus from Meliotus denatus. Rec Nat Prod 3(2):114–117Google Scholar
  27. Jallow MFA, Dugassa-Gobena D, Vidal S (2008) Influence of an endophytic fungus on host plant selection by a polyphagous moth via volatile spectrum changes. Arthropod–Plant Integr 2:53–62CrossRefGoogle Scholar
  28. Kaul S, Gupta S, Ahmed M, Dhar M (2012) Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem Rev 11:487–505CrossRefGoogle Scholar
  29. Kavroulakis NS, Zervakis GI, Ehaliotis C, Haralampidis K, Papadopoulou KK (2007) Role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic Fusarium solani strain. J Exp Bot 58:3853–3864CrossRefPubMedGoogle Scholar
  30. Kharwar RN, Mishra A, Gond SK, Stierle A, Stierle D (2011) Anticancer compounds derived from fungal endophytes: their importance and future challenges. Nat Prod Rep 28:1208–1288CrossRefPubMedGoogle Scholar
  31. Kumar S, Kaushik N (2013) Endophytic fungi isolated from oil-seed crop Jatropha curcas produces oil and exhibit antifungal activity. PLoS One 8(2):e56202. doi: 10.1371/journal.Pone.0056202PMID: 23409154 CrossRefPubMedPubMedCentralGoogle Scholar
  32. Kusari S, Singh S, Jayabaskaran C (2015) Rethinking production of Taxol® using endophyte biotechnology.
  33. Lacey LA, Horton DR, Jones DC, Headrick HL, Neven LG (2009) Efficacy of the biofumigant fungus Muscodor albus (Ascomycota: Xylariales) for control of codling moth (Lepidoptera: Tortricidae) in simulated storage conditions. J Econ Entomol 102(1):43–49CrossRefPubMedGoogle Scholar
  34. Larkin BG, Hunt LS, Ramsey PW (2012) Foliar nutrients shape fungal endophyte communities in Western white pine (Pinus monticola) with implications for white-tailed deer herbivory. Fungal Ecol 5(2):252–260CrossRefGoogle Scholar
  35. Latch GCM (1993) Physiological interactions of endophytic fungi and their hosts: biotic stress tolerance imparted to grasses by endophytes. Agric Ecosyst Environ 44:143–156CrossRefGoogle Scholar
  36. Link HF (1809) Observationes in ordines plantarum naturales, dissertatioprima, complectens anandrarumordinesEpiphytas, Mucedines, Gastromycoset Fungos. DerGesellschaftNaturforschenderFreundezuBerlin, BerlinGoogle Scholar
  37. Liu S, Ruan W, Li J, Xu H, Wang J, Gao Y et al (2008) Biological control of phytopathogenic fungi by fatty acids. Mycopathologia 166(2):93–102. doi: 10.1007/s11046-008-9124-1PMID:18443921 84 CrossRefPubMedGoogle Scholar
  38. Loro M, Valero-Jiménez CA, Nozawa S, Márquez LM (2012) Diversity and composition of fungal endophytes in semiarid Northwest Venezuela. J Arid Environ 85:46–55CrossRefGoogle Scholar
  39. Lu H, Zou WX, Meng JC, Hu J, Tan RX (2000) New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Sci 151:67–73CrossRefGoogle Scholar
  40. Mejıa LC, Rojas EI, Maynard Z, Bael SV, Elizabeth Arnold A, Hebbar P, Samuels GJ, Robbins N, Herre EA (2008) Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biol Control 46:4–14CrossRefGoogle Scholar
  41. Miller JD, MacKenzie S, Foto M, Adams GW, Findlay JA (2002) Needles of white spruce inoculated with rugulosin producing endophytes contain rugulosin reducing spruce budworm growth rate. Mycol Res 106:471–479CrossRefGoogle Scholar
  42. Monali, Bodhankar (2014) Antimicrobial activity of endophytic fungi isolated from Ocimum sanctum Linn. Am J Cont Sci Res 1(3) ISSN 2349-4425Google Scholar
  43. Nuryanti WH (2015) Screening of volatile compounds of Brotowali (Tinospora Crispa) and antifungal activity against Candida albicans. Int J Pharma Phytochem Res 7(1);132–136. 85Google Scholar
  44. Oliveira CM, Silva GH, Regasini LO, Zanardi LM, Evangelista AH, Young MC, Bolzani VS, Araujo AR (2009) Bioactive metabolites produced by Penicillium sp.1 and sp.2, two endophytes associated with Alibertia macrophylla. Z Naturforsch C 64(11–12):824–830PubMedGoogle Scholar
  45. Pal KK, Gardener BM (2006) Biological control of plant pathogens. Plant Health Instr. doi: 10.1094/PHI-A-2006–1117–02
  46. Pavithra N, Sathish L, Suneel Kumar A, Venkatarathanamma V, Pushpalatha H, Bhanuprakash RG, Ananda K (2016) In-vitro studies on α-amylase, α-glucosidase and aldose reductase inhibitors found in endophytic fungi isolated from Ocimum sanctum. Curr Enzym Inhib 10(2):129–136Google Scholar
  47. Petrini O (1991) Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds) Microbial ecology of leaves. Springer, New York, pp 179–197. CrossRefGoogle Scholar
  48. Pu X, Qu X, Chen F, Bao J, Zhang G, Luo Y (2013) Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production. Appl Microbiol Biotechnol 97(21):9365–9375. doi: 10.1007/s00253-013-5163-8 CrossRefPubMedGoogle Scholar
  49. Redman RS, Sheehan KB, Stout TG, Rodríguez RJ, Henson JM (2002) Thermotolerance generated by plant/fungal symbiosis. Science 298:1581CrossRefPubMedGoogle Scholar
  50. Reiter B, Pfeifer U, Schwab H, Sessitsch A (2002) Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica. Appl Environ Microbiol 68:2261–2268. CrossRefPubMedPubMedCentralGoogle Scholar
  51. Richardson MD, Chapman GW, Hoveland CS, Bacon CW (1992) Sugar alcohols in endophyte-infected tall fescue. Crop Sci 32:1060–1061CrossRefGoogle Scholar
  52. Rodriguez RJ, White JF, Arnold AE, Redman RS (2009) Fungal endophytes: diversity and functional roles. New Phytol 182:314–330. http: // CrossRefPubMedGoogle Scholar
  53. Rohlfs M, Churchill ACL (2011) Fungal secondary metabolites as modulators of interactions with insects and other arthropods. Fungal Genet Biol 48:23–34CrossRefPubMedGoogle Scholar
  54. Rosenberg E, Sharon G, Zilber-Rosenberg I (2009) The hologenome theory of evolution contains Lamarckian aspects within a Darwinian framework. Environ Microbiol 11:2959–2962. Scholar
  55. Sánchez Márquez S, Bills GF, Herrero N, Zabalgogeazcoa I (2012) Non-systemic fungal endophytes of grasses. Fungal Ecol 5:289–297CrossRefGoogle Scholar
  56. Schafer P, Khatabi B, Kogel KH (2007) Root cell death and systemic effects of Piriformospora indica: a study on mutualism. FEMS Microbiol Lett 275:1–7CrossRefPubMedGoogle Scholar
  57. Schulz B, Boyle C (2005) The endophytic continuum. Mycol Res 109:661–687CrossRefPubMedGoogle Scholar
  58. Schulz S, Keatinge JDH, Wells GJ (1999) Productivity and residual effects of legumes in rice-based cropping systems in a warm-temperate environment. Field Crop Res 61:37–49CrossRefGoogle Scholar
  59. Senthilkumar M, Anandham R, Madhaiyan M, Venkateswaran V, Tongmin S (2011) Endophytic bacteria: perspectives and applications in agricultural crop production. In: Maheshwari DK (ed) Bacteria in agrobiology: crop ecosystems. Springer Verlag, Berlin, pp 61–96CrossRefGoogle Scholar
  60. Senthilkumar N, Murugesan S, Babu DS (2014) Metabolite profiling of the extracts of endophytic fungi of entomopathogenic significance, Aspergillus flavus and Nigrospora sphaerica isolated from tropical tree species of India, Tectona grandis L. J Agric Life Sci 1(1):108–114Google Scholar
  61. Sharma, Kumar (2013) Isolation, characterization and antioxidant potential of endophytic fungi of Ocimum sanctum Linn. Indian J Appl Res 3(7) ISSN-2249-555XGoogle Scholar
  62. Shukla ST, Kulkarni VH, Habbu PV, Jagadeesh KS, Patil BS, Smita DM (2012) Hepatoprotective and antioxidant activities of crude fractions of endophytic fungi of O. sanctum Linn in rats. Orient Pharma Exp Med 12:81–91CrossRefGoogle Scholar
  63. Stierle A, Strobel G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae and endophytic fungus of Pacific yew. Science 260:214–221CrossRefPubMedGoogle Scholar
  64. Strobel GA (2002) Rainforest endophytes and bioactive products. Crit Rev Biotechnol 22(4):315–333CrossRefPubMedGoogle Scholar
  65. Strobel G, Singh SK, Riyaz-Ul-Hassan S, Mitchell AM, Geary B, Sears J (2011) An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential. FEMS Microbiol Lett 320(2):87–94. doi: 10.1111/j.1574-6968.2011.02297.x CrossRefPubMedGoogle Scholar
  66. Sun C, Johnson JM, Cai D, Sherameti I, Oelmüller R, Lou B (2010) Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastid-localized CAS protein. J Plant Physiol 167:10091017Google Scholar
  67. Tian P, Nan Z, Li C (2008) Effect of the endophyte Neotyphodium lolii on susceptibility and host physiological response of perennial ryegrass to fungal pathogens. Eur J Plant Pathol 122:593–602CrossRefGoogle Scholar
  68. Trivedi DK, Ansari MW, Tuteja N (2014a) Response of PiCypA tobacco T2 transgenic matured plant to potential tolerance to salinity stress. Plant Signal Behav 8:e27538CrossRefGoogle Scholar
  69. Trivedi DK, Ansari MW, Dutta T, Singh P, Tuteja N (2014b) Molecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli. BMC Res Notes 23(6):555Google Scholar
  70. Varma A, Bajaj R, Agarwal A, Asthana S, Rajpal K, Das A, Prasad R, Kharkwal AC (2013) Memoirs of ‘Rootonic’-the magic fungus. Amity University Press, NoidaGoogle Scholar
  71. Vega FE, Posada F, Aime MC, Pava-Ripoll M, Infante F, Rehner SA (2008) Entomopathogenic fungal endophytes. Biol Control 46:7282CrossRefGoogle Scholar
  72. Verma VC, Lobkovsky E, Gange AC, Singh SK, Prakash S (2011) Piperine production by endophytic fungus Periconia sp. isolated from Piper longum L. J Ant 64:427–431CrossRefGoogle Scholar
  73. Vuorela H (2004) Natural products in the process of finding new drug candidates. Curr Med Chem 11:1375–1389CrossRefPubMedGoogle Scholar
  74. Waller F, Baltruschat H, Achatz B, Becker K, Fischer M, Fodor J, Heier T, Huckelhoven R, Neumann C (2005) The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance and higher yield. Proc Natl Acad Sci U S A 102(38):13386–13391CrossRefPubMedPubMedCentralGoogle Scholar
  75. Wang Y, Zeng QG, Zhang ZB, Yan RM, Wang LY, Zhu D (2011) Isolation and characterization of endophytic huperzine A producing fungi from Huperzia serrata. J Ind Microbiol Biotechnol 38:1267–1278CrossRefPubMedGoogle Scholar
  76. White JF Jr, Torres MS (2010) Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection? Physiol Plant 138(4):440–446CrossRefPubMedGoogle Scholar
  77. Wilson D (1995) Endophyte-the evolution of a term, a clarification of its use and definition. Oikos 73:274–276CrossRefGoogle Scholar
  78. Wu SH, Chen YW, Shao SC, Wang LD, Yu Y, Li ZY, Yang LY, Li SL, Huang R (2008) Two new solanapyronean alogues from the endophytic fungus Nigrospora sp. YB-141 of Azadirachta indica. Chem Biodivers 6(1):79–85CrossRefGoogle Scholar
  79. Xiao X, Luo SL, Zeng G, Wei W, Wan Y, Chen L, Guo H, Cao Z, Yang L, Chen J, Xi Q (2010) Biosorption of cadmium by endophytic fungus (EF) Microsphaeropsis sp. LSE10 isolated from cadmium hyperaccumulator Solanum nigrum L. Bioresour Technol 101(6):1668–1674CrossRefPubMedGoogle Scholar
  80. Yadav R, Singh AV, Joshi S, Kumar M (2015) Antifungal and enzyme activity of endophytic fungi isolated from O. sanctum and Aloe vera. Afr J Microbiol Res 9(29):1783–1788CrossRefGoogle Scholar
  81. Yang X, Guo S, Zhang L, Shao H (2003) Selection of producing podophyllotoxin endophytic fungi from podophyllin plant. Nat Prod Res Devpt 15:419–422Google Scholar
  82. Zhang L, Guo B, Li H, Zeng S, Shao H, Go S, Wei R (2000) Preliminary study on the isolation of endophytic fungus of Catharanthus roseus and its fermentation to produce products of therapeutic value. Chin Trad Herbal Drugs 31:805–807Google Scholar
  83. Zhang HW, Song YC, Tan RX (2006) Biology and chemistry of endophytes. Nat Prod Rep 23(5):753–771Google Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Centre for Rural Development and TechnologyIndian Institute of Technology-DelhiNew DelhiIndia

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