Abstract
Roots and pods of field-grown peanut were sampled at three developmental stages and a total of seven bacterial strains and one toxigenic Aspergillus flavus isolates were isolated from the geocarposphere. The biocontrol potential of each bacterial strain was tested against growth and Afl-B1 production potential of toxigenic A. flavus. In greenhouse experiments, co-inoculation with toxigenic A. flavus and geocarposphere bacteria in root regions of 1- to 2-week-old peanut plants resulted in the lower synthesis of Afl-B1 in the peanut kernels at maturity. Of the seven bacterial strains tested, four strains showed a reduction in aflatoxins production in varying extents. Pre-inoculation of bacterial strains (1 day earlier) resulted in greater inhibition of aflatoxin accumulation than those bacterial strains introduced 1 day after inoculation of toxigenic A. flavus strain. Bacillus megaterium showed maximum inhibition of aflatoxin biosynthesis, as compared to remaining three potential bacterial strains.
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Acknowledgements
The authors are thankful to Prof. Barry J. Jacobsen, Head, Department of Plant Pathology, Alabama Agricultural Experiment Station, Auburn University, USA, for providing laboratory facilities. Financial support to HKC by Govt. of India, Dept. of Education, New Delhi in the form of Postdoctoral Fellowship is gratefully acknowledged.
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Chourasia, H.K., Sah, P.K. (2017). Control of Aflatoxin Biosynthesis in Peanut with Geocarposphere Bacteria: A Biotechnological Approach for Sustainable Development. In: Mukhopadhyay, K., Sachan, A., Kumar, M. (eds) Applications of Biotechnology for Sustainable Development. Springer, Singapore. https://doi.org/10.1007/978-981-10-5538-6_9
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DOI: https://doi.org/10.1007/978-981-10-5538-6_9
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