Abstract
Quorum sensing (QS) systems allow bacterial organisms to coordinate their behavior depending on the local population density and are used in many artificial systems requiring cell-to-cell communication. The accumulation of optimum stimulatory levels of autoinducers is detected by bacteria that affect the gene expression of their behavior. QS bacteria defer the production of virulence factors until cell numbers reach optimum levels, resulting in infection leading to activation of the host immune system by secretion of virulence factors causing productive infection. It is a successful technique that coordinates the gene expression of groups of organisms. In this chapter, the mechanisms pertaining to varied bacterial QS systems are presented and discussed. The differences between two definitive bacterial signal transduction systems are also discussed. We contend that the bacterial QS systems are optimally intended to specifically translate extracellular autoinducer information into internal changes via gene expression. In addition, the different bacterial QS systems used in deciphering basic mechanisms underlying the advancement of bacterial communities are discussed. Here, we review the updated advancements of the genetic approaches in engineering QS circuits to utilize bacterial communication in environmental biotechnology.
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References
Ansaldi M, Marolt D, Stebe T, Mandic-Mulec I, Dubnau D (2002) Specific activation of the Bacillus quorum- sensing systems by isoprenylated pheromone variants. Mol Microbiol 44:1561–1573
Balaban N, Novick RP (1995) Translation of RNAIII, the Staphylococcus aureus agr regulatory RNA molecule, can be activated by a 39-end deletion. FEMS Microbiol Lett 133:155–161
Bendiak GN, Ratjen F (2009) The approach to Pseudomonas aeruginosa in cystic fibrosis. Semin Respir Crit Care Med 30:587–595
Benito Y, Kolb FA, Romby P, Lina G, Etienne J, Vandenesch F (2000) Probing the structure of RNAIII, the Staphylococcus aureus agr regulatory RNA, and identification of the RNA domain involved in repression of protein A expression. RNA 6:668–679
Bodey GP, Bolivar R, Fainstein V, Jadeja L (1983) Infections caused by Pseudomonas aeruginosa. Rev Infect Dis 5:279–313
Booth MC, Bogie CP, Sahl HG, Siezen RJ, Hatter KL, Gilmore MS (1996) Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibiotic. Mol Microbiol 21:1175–1184
Booth MC, Cheung AL, Hatter KL, Jett BD, Callegan MC, Gilmore MS (1997) Staphylococcal accessory regulator (sar) in conjunction with agr contributes to Staphylococcus aureus virulence in endophthalmitis. Infect Immun 65:1550–1556
Bowden MG, Chen W, Singvall J, Xu Y, Peacock SJ, Valtulina V, Speziale P, Hook M (2005) Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis. Microbiology 151:1453–1464
Chater KF, Horinouchi S (2003) Signaling early developmental events in two highly diverged Streptomyces species. Mol Microbiol 48:9–15
Cheung AL, Eberhardt KJ, Chung E, Yeaman MR, Sullam PM, Ramos M, Bayer AS (1994) Diminished virulence of a sar2/agr2 mutant of Staphylococcus aureus in the rabbit model of endocarditis. J Clin Invest 94:1815–1822
Chugani SA, Whiteley M, Lee KM, D’Argenio D, Manoil C, Greenberg EP (2001) QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 98:2752–2757
Cirioni O, Ghiselli R, Minardi D, Orlando F, Mocchegiani F, Silvestri C, Muzzonigro G, Saba V, Scalise G (2007) RNAIII-inhibiting peptide affects biofilm formation in a rat model of staphylococcal ureteral stent infection. Antimicrob Agents Chemother 51:4518–4520
Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP (1998) The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295–298
De Kievit TR, Iglewski BH (2000) Bacterial quorum sensing in pathogenic relationships. Infect Immun 68:4839–4849
DiMango E, Zar HJ, Bryan R, Prince A (1995) Diverse Pseudomonas aeruginosa gene products stimulate respiratory epithelial cells to produce interleukin-8. J Clin Invest 96:2204–2210
Driscoll JA, Brody SL, Kollef MH (2007) The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 67:351–368
Dufour P, Jarraud S, Vandenesch F, Greenland T, Novick RP et al (2002) High genetic variability of the agr locus in staphylococcus species. J Bacteriol 184:1180–1186
Eberhard A, Burlingame AL, Eberhard C, Kenyon GL, Nealson KH, Oppenheimer NJ (1981) Structural identification of autoinducer of Photobacterium fischeri luciferase. Biochemistry 20:2444–2449
Engebrecht J, Silverman M (1984) Identification of genes and gene products necessary for bacterial bioluminescence. Proc Natl Acad Sci U S A 81:4154–4158
Engebrecht J, Nealson K, Silverman M (1983) Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri. Cell 32:773–781
Erickson DL, Endersby R, Kirkham A, Stuber K, Vollman DD, Rabin HR, Mitchell I, Storey DG (2002) Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis. Infect Immun 70:1783–1790
Fleming V, Feil E, Sewell AK, Day N, Buckling A, Massey RC (2006) Agric interference between clinical Staphylococcus aureus strains in an insect model of virulence. J Bacteriol 188:7686–7688
Fuqua C (2006) The QscR quorum-sensing regulon of Pseudomonas aeruginosa: an orphan claims its identity. J Bacteriol 188:3169–3171
Fuqua C, Eberhard A (1999) Signal generation in autoinduction systems: synthesis of acylated homoserine lactones by LuxI-type proteins. In: Dunny GM, Winans SC (eds) Cell-cell signaling in bacteria. ASM Press, Washington, DC, pp 211–230
George EA, Muir TW (2007) Molecular mechanisms of agr quorum sensing in virulent staphylococci. ChemBio 8:847–855
Gillaspy AF, Hickmon SG, Skinner RA, Thomas JR, Nelson CL, Smeltzer MS (1995) Role of the accessory gene regulator (agr) in pathogenesis of staphylococcal osteomyelitis. Infect Immun 63:3373–3380
Goerke C, Kraning K, Stern M, Doring G, Botzenhart K, Wolz C (2000) Molecular epidemiology of community-acquired Staphylococcus Aureus in families with and without cystic fibrosis patients. J Infect Dis 181:984–989
Gould TA, Schweizer HP, Churchill ME (2004) Structure of the Pseudomonas aeruginosa acylhomoserinelactone synthase LasI. Mol Microbiol 53:1135–1146
Grumbein S, Opitz M, Lieleg O (2014) Selected metal ions protect Bacillus subtilis biofilms from erosion. Metallomics 6:1441–1450
Heurlier K, Denervaud V, Haas D (2006) Impact of quorum sensing on fitness of Pseudomonas aeruginosa. Int J Med Microbiol 296:93–102
Heyer G, Saba S, Adamo R, Rush W, Soong G, Cheung A, Prince A (2002) Staphylococcus aureus agr and sarA functions are required for invasive infection but not inflammatory responses in the lung. Infect Immun 70:127–133
Ivanova K, Fernandes MM, Tzanov T (2013) Current advances on bacterial pathogenesis inhibition and treatment strategies. In: Méndez-Vilas A (eds) Microbial pathogens and strategies for combating them: science, technology and education. Formatex Research Center, pp 322–336. https://doi.org/10.13140/RG.2.1.3988.7840
Ji G, Beavis RC, Novick RP (1995) Cell density control of staphylococcal virulence mediated by an octapeptide pheromone. Proc Natl Acad Sci U S A 92:12055–12059
Ji G, Beavis R, Novick RP (1997) Bacterial interference caused by auto inducing peptide variants. Science 276:2027–2030
Kaplan HB, Greenberg EP (1985) Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminescence system. J Bacteriol 163:1210–1214
Karatuna O, Yagci A (2010) Analysis of the quorum sensing dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates. Clin Microbiol Infect. https://doi.org/10.1111/j.1469-0691.2010.03177.x
Kehinde EO, Rotimi VO, Al-Hunayan A, Abdul-Halim H, Boland F, Al-Awadi KA (2004) Bacteriology of urinary tract infection associated with indwelling J ureteral stents. J Endourol 18:891–896
Kielian T, Cheung A, Hickey WF (2001) Diminished virulence of an a-toxin mutant of Staphylococcus aureus in experimental brain abscesses. Infect Immun 69:6902–6911
Koenig RL, Ray JL, Maleki SJ, Smeltzer MS, Hurlburt BK (2004) Staphylococcus aureus AgrA binding to the RNAIII-agr regulatory region. J Bacteriol 186:7549–7555
Kohler T, Buckling A, Van DC (2009) Cooperation and virulence of clinical Pseudomonas aeruginosa populations. Proc Natl Acad Sci U S A 106:6339–6344
Kong KF, Vuong C, Otto M (2006) Staphylococcus quorum sensing in biofilm formation and infection. Int J Med Microbiol 296:133–139
Kravchenko VV, Kaufmann GF, Mathison JC, Scott DA, Katz AZ, Grauer DC, Lehmann M, Meijler MM, Janda KD, Ulevitch RJ (2008) Modulation of gene expression via disruption of NF-kB signaling by a bacterial small molecule. Science 321:259–263
Latifi A, Foglino M, Tanaka K, Williams P, Lazdunski A (1996) A hierarchical quorum-sensing cascade in pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary-phase sigma factor RpoS. Mol Microbiol 21:1137–1146
Lauderdale KJ, Boles BR, Cheung AL, Horswill AR (2009) Interconnections between sigma B, agr, and proteolytic activity in Staphylococcus aureus biofilm maturation. Infect Immun 77:1623–1635
Lina G, Jarraud S, Ji G, Greenland T, Pedraza A, Etienne J, Novick RP, Vandenesch F (1998) Transmembrane topology and histidine protein kinase activity of AgrC, the agr signal receptor in Staphylococcus aureus. Mol Microbiol 28:655–662
Lyczak JB, Cannon CL, Pier GB (2000) Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect 2:1051–1060
Lyon GJ, Novick RP (2004) Peptide signaling in Staphylococcus aureus and other Gram-positive bacteria. Peptides 25:1389–1403
Lyon GJ, Wright JS, Muir TW, Novick RP (2002) Key determinants of receptor activation in the agr autoinducing peptides of Staphylococcus aureus. Biochemistry 41:10095–10104
Manefield M, Turner SL (2002) Quorum sensing in context: out of molecular biology and into microbial ecology. Microbiology 148:3762–3764
Marketon MM, Gronquist MR, Eberhard A, Gonzalez JE (2002) Characterization of the Sinorhizobium meliloti sinR/sinI locus and the production of novel N-acyl homoserine lactones. J Bacteriol 184:5686–5695
Massey RC, Horsburgh MJ, Lina G, Hook M, Recker M (2006) The evolution and maintenance of virulence in Staphylococcus aureus: a role for host-to-host transmission. Nat Rev Microbiol 4:953–958
Mayville P, Ji G, Beavis R, Yang H, Goger M, Novick RP, Muir TW (1999) Structure-activity analysis of synthetic autoinducing thiolactone peptides from Staphylococcus aureus responsible for virulence. Proc Natl Acad Sci U S A 96:1218–1223
Mishler DM, Topp S, Reynoso CMK, Gallivan JP (2010) Engineering bacteria to recognize and follow small molecules. Curr Opin Biotechnol 21(5):653–656. https://doi.org/10.1016/j.copbio.2010.05.007
More MI, Finger LD, Stryker JL, Fuqua C, Eberhard A, Winans SC (1996) Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates. Science 272:1655–1658
Morfeldt E, Taylor D, Von GA, Arvidson S (1995) Activation of a-toxin translation in Staphylococcus aureus by the trans encoded antisense RNA, RNAIII. EMBO J 14:4569–4577
Mulcahy H, Charron-Mazenod L, Lewenza S (2010) Pseudomonas aeruginosa produces an extracellular deoxyribonuclease that is required for utilization of DNA as a nutrient source. Environ Microbiol 12:1621–1629
Nakayama J, Cao Y, Horii T, Sakuda S, Akkermans AD, de Vos WM, Naqasawa H (2001) Gelatinase biosynthesisactivating pheromone: a peptide lactone that mediates a quorum sensing in enterococcus faecalis. Mol Microbiol 41:145–154
Nealson KH, Hastings JW (1979) Bacterial bioluminescence: its control and ecological significance. Microbiol Rev 43:496–518
Novick RP (2003) Autoinduction and signal transduction in the regulation of staphylococcal virulence. Mol Microbiol 48:1429–1449
Novick RP, Geisinger E (2008) Quorum sensing in staphylococci. Annu Rev Genet 42:541–564
Novick RP, Ross HF, Projan SJ, Kornblum J, Kreiswirth B, Moghazeh S (1993) Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 12:3967–3975
Novick RP, Projan SJ, Kornblum J, Ross HF, Ji G, Kreiswirth B, Vandenesch F, Moghazeh S (1995) The agr P2 operon: an autocatalytic sensory transduction system in Staphylococcus aureus. Mol Gen Genet 248:446–458
Otto M, Sussmuth R, Jung G, Gotz F (1998) Structure of the pheromone peptide of the Staphylococcus epidermidis agr system. FEBS Lett 424:89–94
Parsek MR, Tolker-Nielsen T (2008) Pattern formation in Pseudomonas aeruginosa biofilms. Curr Opin Microbiol 11:560–566
Parsek MR, Val DL, Hanzelka BL, Cronan JE, Greenberg EP (1999) Acyl homoserinelactone quorum-sensing signal generation. Proc Natl Acad Sci U S A 96:4360–4365
Pearson JP, Van Delden C, Iglewski BH (1999) Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals. J Bacteriol 181:1203–1210
Roux A, Payne SM, Gilmore MS (2009) Microbial telesensing: probing the environment for friends, foes, and food. Cell Host Microbe 6:115–124
Saenz HL, Augsburger V, Vuong C, Jack RW, Gotz F, Otto M (2000) Inducible expression and cellular location of AgrB, a protein involved in the maturation of the staphylococcal quorum-sensing pheromone. Arch Microbiol 174:452–455
Sandoz KM, Mitzimberg SM, Schuster M (2007) Social cheating in Pseudomonas aeruginosa quorum sensing. Proc Natl Acad Sci U S A 104:15876–15881
Schu DJ, Scruggs JM, Geissinger JS, Michel KG, Stevens AM (2014) Acyl-homoserine lactone recognition and response hindering the quorum-sensing regulator EsaR. PLoS One 9:e107687
Schuster M, Greenberg EP (2006) A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa. Int J Med Microbiol 296:73–81
Schuster M, Lostroh CP, Ogi T, Greenberg EP (2003) Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis. J Bacteriol 185:2066–2079
Smith RS, Iglewski BH (2003) P. aeruginosa quorum-sensing systems and virulence. Curr Opin Microbiol 6:56–60
Smith RS, Harris SG, Phipps R, Iglewski B (2002) The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl) homoserine lactone contributes to virulence and induces inflammation in vivo. J Bacteriol 184:1132–1139
Steinmoen H, Teigen A, Havarstein LS (2003) Competence-induced cells of Streptococcus pneumoniae lyse competence-deficient cells of the same strain during cocultivation. J Bacteriol 185:7176–7183
Stevens AM, Dolan KM, Greenberg EP (1994) Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region. Proc Natl Acad Sci U S A 91:12619–12623
Tenover FC, Gaynes RP (2000) The epidemiology of Staphylococcus aureus infections. In: Fischetti VA, Novick RP, Ferretti JJ, Portnoy DA, Rood JI (eds) Gram- positive pathogens. ASM Press, Washington, DC, pp 414–421
Vannini A, Volpari C, Gargioli C, Muraglia E, Cortese R, De Francesco R, Neddermann P, Marco SD (2002) The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA. EMBO J 21:4393–4401
Visick KL, Foster J, Doino J, McFall-Ngai M, Ruby EG (2000) Vibrio fischeri lux genes play an important role in colonization and development of the host light organ. J Bacteriol 182:4578–4586
Vuong C, Saenz HL, Gotz F, Otto M (2000) Impact of the agr quorum-sensing system on adherence to polystyrene in Staphylococcus aureus. J Infect Dis 182:1688–1693
Watson WT, Minogue TD, Val DL, Von Bodman SB, Churchill ME (2002) Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing. Mol Cell 9:685–694
Whitehead NA, Barnard AM, Slater H, Simpson NJ, Salmond GP (2001) Quorum-sensing in Gram-negative bacteria. FEMS Microbiol Rev 25:365–404
Wright JS, Traber KE, Corrigan R, Benson SA, Musser JM, Novick RP (2005) The agr radiation: an early event in the evolution of staphylococci. J Bacteriol 187:5585–5594
Yao Y, Vuong C, Kocianova S, Villaruz AE, Lai Y, Sturdevant DE, Otto M (2006) Characterization of the Staphylococcus epidermidis accessory-gene regulator response: quorum-sensing regulation of resistance to human innate host defense. J Infect Dis 193:841–848
Yarwood JM, Bartels DJ, Volper EM, Greenberg EP (2004) Quorum sensing in Staphylococcus aureus biofilms. J Bacteriol 186:1838–1850
Yoon SS, Hennigan RF, Hilliard GM, Ochsner UA, Parvatiyar K, Kamani MC, Allen HL, DeKievit TR, Gardner PR, Schwab U, Rowe JJ, Iglewski BH, McDermott TR, Mason RP, Wozniak DJ, Hancock RE, Parsek MR, Noah TL, Boucher RC, Hassett DJ (2002) Pseudomonas aeruginosa anaerobic respiration in biofilms: relationships to cystic fibrosis pathogenesis. Dev Cell 3:593–603
Zhang RG, Pappas T, Brace JL, Miller PC, Oulmassov T, Molyneaux JM, Anderson JC, Bashkin JK, Winans SC, Joachimiak A (2002) Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature 417:971–974
Zhang L, Lin J, Ji G (2004) Membrane anchoring of the AgrD Nterminal amphipathic region is required for its processing to produce a quorum-sensing pheromone in Staphylococcus aureus. J Biol Chem 279:19448–19456
Zhu J, Winans SC (1999) Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells. Proc Natl Acad Sci U S A 96:4832–4837
Zhu J, Winans SC (2001) The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding, protease resistance, and dimerization. Proc Natl Acad Sci U S A 98:1507–1512
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Parray, J.A., Jan, S., Mir, M.Y., Shameem, N., Kamili, A.N. (2018). Quorum Sensing: Melody Beneath the Ground. In: Egamberdieva, D., Ahmad, P. (eds) Plant Microbiome: Stress Response. Microorganisms for Sustainability, vol 5. Springer, Singapore. https://doi.org/10.1007/978-981-10-5514-0_9
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