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Pretreatment and Production of Bioethanol from Citrus reticulata Fruit Waste with Baker’s Yeast by Solid-State and Submerged Fermentation

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Utilization and Management of Bioresources

Abstract

Excessive use of fossil fuels leads the world to investigate the other alternative sources of energy. Biofuels are considered as one of the emerging and more reliable energy sources. The present study investigates the potential of Citrus reticulata (orange) fruit waste for efficient production of bioethanol.

During the study orange peel and pulp waste samples in the ratio of 1:1 were pretreated to acidic and heat treated followed by solid-state and submerged fermentation. Fermented fractions were then subjected to distillation. Distilled fermented fractions were then analyzed by gas chromatography-mass spectrometry (GC-MS). The chromatographic analysis revealed that from Citrus reticulata fruit wastes, biodiesel can be produced, and the highest bioethanol yield (6.0029%) was observed via solid-state fermentation compared to submerged fermentation. Positive and promising results of GC-MS show that Citrus reticulata fruit wastes can be a feasible alternative for efficient bioethanol production.

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References

  • Asgher M, Bashir F, Iqbal HMN (2014) A comprehensive ligninolytic pre-treatment approach from lignocellulose green biotechnology to produce bio-ethanol. Chem Eng Res Des 92:1571–1157

    Article  CAS  Google Scholar 

  • Chuan-Liang Hsu, Ku-Shang Chang, Ming-Zhang Lai, Tsan-Chang Chang, Yi-Huang Chang, Hung-Der Jang (2011) Pretreatment and hydrolysis of cellulosic agricultural wastes with a cellulase-producing Streptomyces for bioethanol production. Biomass Bioenergy 35(5):1878–1884

    Article  Google Scholar 

  • Citrus: World Markets and Trade (2016) World production, markets, and trade reports web: http://www.fas.usda.gov/data/citrus-world-markets-and-trade

  • Cuevas M, Sánchez S, Bravo V, García JF, Baeza J, Parra C, Freer J (2010) Determination of optimal pre-treatment conditions for ethanol production from olive-pruning debris by simultaneous saccharification and fermentation. Process Biochem 89:2891–2896

    CAS  Google Scholar 

  • Dake M, Jadhv J, Patil N (2010) Variations of two pools of glycogen and carbohydrate in Saccharomyces cerevisiae grown with various ethanol concentrations. J Ind Microbiol Biotechnol 37:701–706

    Google Scholar 

  • Deepa A, Da Costa M, Hriiyia M (2015) Production of bioethanol from Citrus limetta and citrus maxima fruit waste by batch fermentation. Int J Pharma Biosci 6:567–578

    CAS  Google Scholar 

  • Dhillon SS, Gill RK, Gill SS, Singh M (2004) Studies on the utilization of citrus peel for pectinase production using fungus Aspergillus niger. Int J Environ Stud 61:199–210

    Article  CAS  Google Scholar 

  • Eisenhuber K, Krennhuber K, Steinmüller V, Jäger A (2013) Comparison of different pre-treatment methods for separating hemicellulose from straw during lignocellulose bioethanol production. Energy Procedia 40:172–181

    Article  CAS  Google Scholar 

  • Farid T (2008) Ethanol production from cellulosic biomass by encapsulated Saccharomyces cerevisiae. Thesis of University College of Boras, School of Engineering, pp 210–221

    Google Scholar 

  • Hassan Al-Haj Ibrahim (2012) Pretreatment of straw for bioethanol production. Energy Procedia 14:542–551

    Article  Google Scholar 

  • Horticultural Statistics at a Glance (2015) Horticulture statistics division department of agriculture, Cooperation & Farmers Welfare Ministry of Agriculture & Farmers Welfare Government of India. Web: agricoop.nic.in/imagedefault/hortstat_glance.pdf

    Google Scholar 

  • Indian Horticulture Database (2014) web: http://nhb.gov.in/area-pro/NHB_Database_2015.pdf

  • Mark W, Wilbur W, Karel G (2007) Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol. Process Biochem 42:1614–1619

    Article  Google Scholar 

  • McCue P, Shetty K (2005) A model for the involvement of lignin degradation enzymes in phenolic antioxidant mobilization from whole soybean during solid-state bioprocessing by Lentinus edodes. Process Biochem 40:1143–1150

    Article  CAS  Google Scholar 

  • Ming Li, Shengli Si, Bo Hao, Yi Zha, Can Wan, Shufen Hong, Yongbo Kang, Jun Jia, Jing Zhang, Meng Li, Chunqiao Zhao, Yuanyuan Tu, Shiguang Zhou, Liangcai Peng (2014) Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus. Bioresour Technol 169:447–454

    Article  Google Scholar 

  • Mishra J, Kumar D, Samanta S, Vishwakarma MK (2012) A comparative study of ethanol production from various agro residues by using Saccharomyces cerevisiae and Candida albicans. J Yeast Fungal Res 3:12–17

    CAS  Google Scholar 

  • Passoth V, Tabassum MR, Nair HAS, Olstorpe M, Tiukova I, Ståhlberg J (2013) Enhanced ethanol production from wheat straw by integrated storage and pre-treatment (ISP). Enzym Microb Technol 52:105–110

    Article  CAS  Google Scholar 

  • Periyasamy S, Venkatachalam S, Ramasamy S, Srinivasan V (2009) Production of bio-ethanol from sugar molasses using Saccharomyces Cerevisiae. Mod Appl Sci 3(8)

    Google Scholar 

  • Shanmugam P (2009) Production of bio-ethanol from sugar molasses using Saccharomyces cerevisiae. Can Cent Sci Educ 3:32–37

    Google Scholar 

  • Shi J, Sharma-Shivappa RR, Chinn M, Howell N (2009) Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenergy 33:88–96

    Article  CAS  Google Scholar 

  • Singh ARP (2014) A comparative study of bioethanol production ability of Bacillus subtilis and Saccharomyces cerevisiae using banana and Orange peels. Int J Sci Eng Res 5:1–5

    Google Scholar 

  • Singh J, Suhag M, Dhaka A (2015) Augmented digestion of lignocellulose by steam explosion, acid and alkaline pretreatment methods: a review, carbohydrate polymers. Process Biochem 117:624–631

    CAS  Google Scholar 

  • Sørensen A, Teller P, Hilstrøm T, Ahring B (2012) Hydrolysis of miscanthus for bio-ethanol production using dilute acid presoaking combined with wet explosion pre-treatment, enzymatic treatment. Bioresour Technol 99:6602–6607

    Article  Google Scholar 

  • Soudham VP, Brandberg T, Mikkola J-P, Larsson C (2014) Detoxification of acid pretreated spruce hydrolysates with ferrous sulfate and hydrogen peroxide improves enzymatic hydrolysis and fermentation. Bioresour Technol 166:559–565

    Article  CAS  Google Scholar 

  • Statistical Review of World Energy (2015) Web: http://www.bp.com/en/global/corporate/about-bp/energy-economics/statistical-review-of-world-energy.html

  • Steffien D, Aubel I, Bertau M (2014) Enzymatic hydrolysis of pre-treated lignocellulose with Penicillium verruculosum cellulases. J Mol Catal B Enzym 103:29–35

    Article  CAS  Google Scholar 

  • Tesfaw A, Assefa F (2014). Current trends in bioethanol production by Saccharomyces cerevisiae: substrate, inhibitor reduction, growth variables, coculture, and immobilization. Int Sch Res Not 2014, Article ID 532852

    Google Scholar 

  • Von Schenck A, Berglin N, Uusitalo J (2013) Ethanol from Nordic wood raw material by simplified alkaline soda cooking pre-treatment. Appl Energy 102:229–240. 38

    Article  CAS  Google Scholar 

  • Weiyang Zhou, Wilbure Widmer, Karel Grohmann (2007) Economic analysis of ethanol production from citrus peel waste. Fla State Hortic Soc 120:310–315

    Google Scholar 

  • Wilkins M, Widmer W, Grohmann K, Cameron R (2007) Hydrolysis of grapefruit peel waste with cellulase and pectinase enzymes. Bioresour Technol 98(2007):1596–1601

    Google Scholar 

  • Ylitervo P (2008) Production of ethanol and biomass from orange peel waste by Mucor indicus. Thesis of University College of Boras (School of Engineering), pp 181–300

    Google Scholar 

  • Young Hoon Jung, Kyoung Heon Kim (2015) Acidic pretreatment, pretreatment of biomass. Chapter 3 Book Korea University Graduate School, Seoul, Republic of Korea, pp 27–50

    Google Scholar 

  • Young Hoon Jung, In Jung Kim, Hyun Kyung Kim, Kyoung Heon Kim (2013) Dilute acid pretreatment of lignocellulose for whole slurry ethanol fermentation. Bioresour Technol 132:109–114

    Article  Google Scholar 

  • Zainab B, Fakhra A (2014) Production of ethanol by fermentation process by using yeast Saccharomyces cerevisiae. Dep Environ Sci 13:24–32

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, PIET, Nagpur, India

    Anup D. Chahande, Pranay A. Raut & Yogesh P. Moharkar

  2. WTM Division, CSIR-NEERI, Nagpur, India

    Vidyadhar V. Gedam

Authors
  1. Anup D. Chahande
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  2. Vidyadhar V. Gedam
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  3. Pranay A. Raut
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  4. Yogesh P. Moharkar
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Corresponding author

Correspondence to Anup D. Chahande .

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Editors and Affiliations

  1. Department of Mechanical Engineering, Jadavpur University, Kolkata, West Bengal, India

    Sadhan Kumar Ghosh

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Chahande, A.D., Gedam, V.V., Raut, P.A., Moharkar, Y.P. (2018). Pretreatment and Production of Bioethanol from Citrus reticulata Fruit Waste with Baker’s Yeast by Solid-State and Submerged Fermentation. In: Ghosh, S. (eds) Utilization and Management of Bioresources. Springer, Singapore. https://doi.org/10.1007/978-981-10-5349-8_13

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