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Long Non Coding RNA Biology pp 283–323Cite as

Technological Developments in lncRNA Biology

Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 1008)

Abstract

It is estimated that more than 90% of the mammalian genome is transcribed as non-coding RNAs. Recent evidences have established that these non-coding transcripts are not junk or just transcriptional noise, but they do serve important biological purpose. One of the rapidly expanding fields of this class of transcripts is the regulatory lncRNAs, which had been a major challenge in terms of their molecular functions and mechanisms of action. The emergence of high-throughput technologies and the development in various conventional approaches have led to the expansion of the lncRNA world. The combination of multidisciplinary approaches has proven to be essential to unravel the complexity of their regulatory networks and helped establish the importance of their existence. Here, we review the current methodologies available for discovering and investigating functions of long non-coding RNAs (lncRNAs) and focus on the powerful technological advancement available to specifically address their functional importance.

Keywords

  • lncRNA
  • Chromatin
  • lncRNA interactions
  • Secondary structure
  • Functional characterization
  • Genome-wide characterization
  • SAGE
  • RNA-Seq
  • CLIP
  • CHART
  • ChIRP

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  1. National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune, 411007, India

    Sonali Jathar, Vikram Kumar, Juhi Srivastava & Vidisha Tripathi

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  2. Vikram Kumar
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    Dr. M.R.S. Rao

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Jathar, S., Kumar, V., Srivastava, J., Tripathi, V. (2017). Technological Developments in lncRNA Biology. In: Rao, M. (eds) Long Non Coding RNA Biology. Advances in Experimental Medicine and Biology, vol 1008. Springer, Singapore. https://doi.org/10.1007/978-981-10-5203-3_10

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