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Selection of Spermatozoa

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Protocols in Semen Biology (Comparing Assays)

Abstract

The isolation of mammalian spermatozoa from the surrounding seminal fluid is a crucial practice commonly applied in assisted reproductive technology applications. The selection of sperm isolation protocol is critically important for investigators, for clinicians preparing sperm samples to be used in reproductive biotechnologies, in veterinary andrology laboratories, and in animal husbandry. Considering the growing importance of the sperm selection techniques, this chapter deals with established sperm selection techniques, viz., simple washing of spermatozoa, swim-up, and discontinuous density-gradient protocol. In addition as a corollary to these protocols, techniques to recover spermatozoa from the epididymis or testicular tissues have been provided alongside.

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Key References

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  2. Hae-Lee Lee and others (2009). See above [18] For excellent presentation of comparison of various protocols for sperm selection using Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for frozen-thawed bovine semen

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Author information

Authors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Megha Pande

  3. Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, India

    S. Tyagi

  4. Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, India

    Omer Din

Authors
  1. N. Srivastava
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  2. Megha Pande
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  3. S. Tyagi
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  4. Omer Din
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Corresponding author

Correspondence to N. Srivastava .

Editor information

Editors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    Megha Pande

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Srivastava, N., Pande, M., Tyagi, S., Din, O. (2017). Selection of Spermatozoa. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_2

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