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Determining Oxidative Stress of Spermatozoa

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Protocols in Semen Biology (Comparing Assays)

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Abstract

Oxidative stress is triggered via numerous reactive oxygen species like peroxides, superoxide anions, nitric oxide, hydroxyl radical and singlet oxygen, which any biological cell/system is unable to counterbalance. In assisted reproductive technologies, the status of seminal oxidative stress can be significantly applied for diagnosing a malady along with prophylactic measures. Accurate oxidative stress measurement may help to develop strategies to reduce oxidative stress during extension and cryopreservation of bovine semen. The present chapter outlines different procedures of measuring oxidative stress in semen samples and their comparative analyses.

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Key Reference

  1. Sanocka and Kurpisz, 2004. See above [48] Provides useful information about interplay among various reactive oxygen species and sperm cells.

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Author information

Authors and Affiliations

  1. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Megha Pande

  2. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    N. Srivastava

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  1. Megha Pande
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  2. N. Srivastava
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Correspondence to Megha Pande .

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Editors and Affiliations

  1. Quality Control, Semen Freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    N. Srivastava

  2. Animal Physiology and Embryo Transfer Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, India

    Megha Pande

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Pande, M., Srivastava, N. (2017). Determining Oxidative Stress of Spermatozoa. In: Srivastava, N., Pande, M. (eds) Protocols in Semen Biology (Comparing Assays). Springer, Singapore. https://doi.org/10.1007/978-981-10-5200-2_12

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