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Advance Techniques in Biophysics

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Introduction to Biomolecular Structure and Biophysics

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Abstract

Solution studies permit a direct investigation of the particles on a well-defined environment. A number of thermodynamic and spectroscopic methods can be used directly in solution providing important structural information. In this work, hydrogen deuterium exchange with mass spectrometry, useful for studying protein interactions, dynamics, and conformations, is explained with data analysis approaches having practical implications. Scattering methods, in particular small-angle X-ray scattering (SAXS), are described, and several applications are presented. As it will be shown, directly from the scattering data, it is possible to obtain size, shape, oligomerization state, and aggregation dynamics among several other parameters. We have further provided the details of cryogenic electron microscopy (“cryo-EM”) where vitreous ice-embedded macromolecules, liposomes, nanoparticles, and three-dimensional structures are determined using several 2D projection images obtained from cryogenic TEM.

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Acknowledgments

CLPO is supported by FAPESP, CNPQ, and INCT-FCx. FCG is supported by FAPESP (grant 2014/17264-3) and CNPq. MF is supported by FAPESP (grant 2012/10862-7).

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Authors and Affiliations

  1. Institute of Chemistry, University of Campinas, Campinas, Brazil

    Mariana Fioramonte & Fabio Cezar Gozzo

  2. Institute of Physics, University of São Paulo, São Paulo, Brazil

    Cristiano Luis Pinto de Oliveira

  3. Brazilian Nanotechnology National Laboratory (LNNano), CNPEM, Campinas, SP, Brazil

    Rodrigo V. Portugal & Marin van Heel

  4. Leiden University – NeCEN, Leiden, The Netherlands

    Marin van Heel

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  3. Cristiano Luis Pinto de Oliveira
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  4. Rodrigo V. Portugal
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  5. Marin van Heel
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Corresponding author

Correspondence to Fabio Cezar Gozzo .

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  1. Amity University, Noida, Uttar Pradesh, India

    Gauri Misra

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Fioramonte, M., Gozzo, F.C., de Oliveira, C.L.P., Portugal, R.V., van Heel, M. (2017). Advance Techniques in Biophysics. In: Misra, G. (eds) Introduction to Biomolecular Structure and Biophysics. Springer, Singapore. https://doi.org/10.1007/978-981-10-4968-2_9

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