Abstract
In this work, the fermentation medium for γ-Aminobutyric acid (GABA) production by Enterococcus raffinosus TCCC11660 in shaken flasks was studied by the Plackett-Burman design. In the preliminary single-factor experiments, sucrose, pyridoxal 5′-phosphate (PLP) and glycine were found to have significant effects on GABA production at the suitable concentration ranges. Subsequently, the screened factors were optimized using Box-Behnken design of response surface methodology. The results showed that the optimal key medium compositions for GABA production by E. raffinosus TCCC11660 were 8.9 g/L citric acid, 0.82 g/L glycine and 0.21 mmol/L PLP, respectively. By fermentation medium optimization, GABA production reached 77.5 g/L, which was enhanced by 103.9% compared to the initial medium.
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Acknowledgements
This work was financially supported by the National Basic Research Program (973 Program) of China (2013CB734004), the National Natural Science Foundation of China (31370075, 31471725 & 61603273), and the Youth Innovation Fund of Tianjin University of Science & Technology of China (2014CXLG28).
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Chang, CY., Ma, SX., Zhang, J., Gao, Q. (2018). Medium Optimization for γ-Aminobutyric Acid Production by Response Surface Methodology. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_41
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