Abstract
A new strain harboring 3-amino-5-hydroxy benzoic acid (AHBA) synthase gene was isolated from China soil and taxonomically was identified as Amycolatopsis kentuckyensis 22-187. It was confirmed that the 22-187 strain mainly produced rifamycin SV and B by chemical identification. The culture medium for Amycolatopsis kentuckyensis 22-187 was optimized on a shake-flask scale by using Plackett-Burman design and response surface methodology for enhanced rifamycin SV production. The Plackett-Burman design indicated that the main factors that positively affect rifamycin SV production by Amycolatopsis kentuckyensis 22-187 were the concentration of glucose and peptone in the fermentation medium. A final concentration of 11.02% (w/v) glucose and 0.98% (w/v) of peptone was found to be best for rifamycin production. Using an optimized fermentation medium (Ammonium Citrate 0.5%, K2HPO4 0.1%, MgSO4 0.1%, CaCO3 0.5%, glucose 11.02%, peptone 0.89%) the titer of fermentation of rifamycin SV was increased two folds comparing with that in the initial medium by HPLC analysis. But the rifamycin B production keeps the same as in initial medium.
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Abbreviations
- AHBA:
-
3-amino-5-hydroxy benzoic acid
- RF:
-
Rifamycins
- RSM:
-
Response surface methodology
- CCD:
-
Central composite design
- ANOVA:
-
Analysis of variance
- PBD:
-
Plackett-Burman design
References
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Acknowledgements
This study was financially supported by the Municipal Science and technology program of Tianjin (Grant number: 12JCYBJC31800). It was also supported by the National High Technology Research and Development Program (2012AA022108) and the program for Changjiang Scholars and Innovative Research Team in University(IRT1166).
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Xu, M., Li, M., Zhang, Y., Zhang, H. (2018). Optimization of Culture Medium for Rifamycin SV Production by Amycolatopsis kentuckyensis 22-187. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_16
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DOI: https://doi.org/10.1007/978-981-10-4801-2_16
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