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Single Cell Analysis pp 39–58Cite as

Rare Cells: Focus on Detection and Clinical Relevance

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Part of the book series: Series in BioEngineering ((SERBIOENG))

Abstract

The study of rare-cell populations is assuming a growing importance to the advancement of medical diagnostics and therapeutics. In several clinical studies, counting rare cells can provide valuable information on the status of the patient; examples are the search for circulating tumor cells in peripheral blood, tumor stem cells, endothelial cells, hematopoietic progenitor cells and their subpopulations, antigen-specific T-cells, invariant natural killer T cells, and fetal cells in maternal circulation. The study of rare-cell populations is useful not only to understand disease mechanisms, but also to find novel targets. With multiparameter capabilities and a very high analysis rate, flow cytometry is at present the most potent technology to address rare-cell analysis. This chapter will describe the main issues of the pre-analytical phase, including the amount of blood to use, the use of pre-enriched populations, the number of markers to use, and the number of cells to acquire. Moreover, we will discuss the importance of excluding doublets and the use of a DUMP channel, along with the importance of using optimal methodologies in all phases, including collection of biological samples, adequate controls, and expert use of software and hardware.

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Authors and Affiliations

  1. Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia School of Medicine, Via Campi, 287-41125, Modena, Italy

    Sara De Biasi, Lara Gibellini & Milena Nasi

  2. Chair of Pathology and Immunology, Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi, 287-41125, Modena, Italy

    Marcello Pinti

  3. Chair of Pathology and Immunology, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Via Campi, 287-41125, Modena, Italy

    Andrea Cossarizza

Authors
  1. Sara De Biasi
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  2. Lara Gibellini
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  3. Milena Nasi
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  4. Marcello Pinti
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  5. Andrea Cossarizza
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Corresponding author

Correspondence to Sara De Biasi .

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Editors and Affiliations

  1. Purdue University Cytometry Laboratories, Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA

    J. Paul Robinson

  2. Chair of Pathology and Immunology, Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia School of Medicine, Modena, Italy

    Andrea Cossarizza

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De Biasi, S., Gibellini, L., Nasi, M., Pinti, M., Cossarizza, A. (2017). Rare Cells: Focus on Detection and Clinical Relevance. In: Robinson, J., Cossarizza, A. (eds) Single Cell Analysis. Series in BioEngineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-4499-1_2

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  • DOI: https://doi.org/10.1007/978-981-10-4499-1_2

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  • Online ISBN: 978-981-10-4499-1

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