Advances in Somatic Cell Reprogramming: Applications in Regenerative Biomedicine and Agriculture

  • N.H. Kieu Linh
  • H-T. Bui
  • N. Van Thuan
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 63)


Dolly the sheep (Wilmut et al in Nature 385:810–813, [1]), the world’s first mammal to be cloned in 1995 from a somatic cell was the greatest scientific achievement of the 20 century, which fundamentally changed the basic knowledge of biologists for somatic cells. In 2006, Shinya Yamanaka made a groundbreaking discovery that adult somatic cells can be reprogrammed to become pluripotent cells by the introduction of four pluripotent genes into somatic cells—so-called induced pluripotent stem (iPS) cells (Takanashi and Yamanaka in Cell 385:810–813, [2]). Those discoveries have opened promising in the research and applications of genomic reprogramming for regenerative biomedicine, biopharmaceutical, stem cell therapy, bio-organ, conservation of the rare and endangered animals, development of transgenic animals for breeding new animal varieties, etc. … Currently, there are three basic methods to reprogram somatic cells into totipotent stem cells or pluripotent stem cells: the first method is somatic cell nuclear transfer (SCNT) by injection of a somatic cell into an enucleated oocyte in order to produce totipotent cell (cloned animals) (Wakayama et al in Nature 394:369–374, [3]), the second is fusion of somatic cell with embryonic stem cells, and the third method is introducing 2 to 4 pluripotent genes, Oct4, Sox2, Klf4 and c-MyC into somatic cell (Takanashi and Yamanaka in Cell 385:810–813, [2]). Recently, we found that treatment of somatic cells with germinal vesicle (GV) oocytes extracts could reprogram somatic cells to stem cells, we named these cells “gviPS” Cells (Bui et al in Development 141:2235–2244, [4]). In the four methods listed above, only SCNT method can reprogram somatic cell into full-term development (offspring), also known as cloning animals. In this presentation we will focus on the most advanced technology in the world and the latest research technologies in animal cloning and the applicability of those technologies in medicine, recombinant human protein applications in pharmaceuticals, and in agriculture. Besides, we will discuss the latest methods that our team has achieved in 20 years of combined 4 key biotechnology 21st century animal cloning techniques re-cell differentiation, gene transfer in higher animals, biotechnology and modern breeding applications in medicine and agriculture.


Somatic cell reprogramming SCNT iPS cells Transgenic animals Pharming 


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This research is funded by Vietnam National University Ho Chi Minh City (VNU-HCM) under grant number B2016-28-01, and by Ministry of Science and Technology of Vietnam under grant number ÐTÐL.CN-49/16.


  1. 1.
    Wilmut AE, Schnieke J McWhir et al (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385:810–813CrossRefGoogle Scholar
  2. 2.
    Takanashi K, Yamanaka S (2006) Introduction of pluripotent stem cell from mouse embryonic and adult fibroblast cultures by defined factors. Cell 385:810–813Google Scholar
  3. 3.
    Wakayama T, Perry ACF, Zuccotti M et al (1998) Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369–374CrossRefGoogle Scholar
  4. 4.
    Bui H-T, Van Thuan N, Kwon D-N et al (2014) Elucidating the identification and proliferation of putative stem cells in the adult pig ovary. Development 141:2235–2244CrossRefGoogle Scholar
  5. 5.
    Kishigami S, Wakayama S, Thuan NV et al (2006) Production of cloned mice by somatic cell nuclear transfer (2006). Nat Protoc 1:125–138CrossRefGoogle Scholar
  6. 6.
    Van Thuan N, Wakayama S, Kishigami S et al (2006) Donor centrosome regulation of initial spindle formation in mouse somatic cell nuclear transfer: roles of gamma-tubulin and nuclear mitotic apparatus protein 1. Biol Reprod 74:777–787CrossRefGoogle Scholar
  7. 7.
    Bui HT, Wakayama S, Kishigami S et al (2010) Effect of trichostatin A on chromatin remodeling, histone modifications, DNA replication, and transcriptional activity in cloned mouse embryos. Biol Reprod 83:454–463CrossRefGoogle Scholar
  8. 8.
    Van Thuan N, Bui Hong-Thuy, Kim JH et al (2009) The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice. Reproduction 2009(85):1048–1056Google Scholar
  9. 9.
    Kishigami S, Mizutani E, Ohta H et al (2006) Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem Biophys Res Commun 340:183–189CrossRefGoogle Scholar
  10. 10.
    Van Thuan N, Kishigami S, Wakayama T (2010) How to improve the success rate of mouse cloning technology. J Reprod Dev 56:20–30CrossRefGoogle Scholar
  11. 11.
    Wakayama S, Kohda T et al (2013) Successful serial recloning in the mouse over multiple generations. CELL (Cell Stem Cell) 12:293–297CrossRefGoogle Scholar
  12. 12.
    Jeon K, Lim H, Van Thuan N et al (2012) Differentiation and transplantation of functional pancreatic beta cells generated from induced pluripotent stem cells derived from a type 1 diabetes mouse model. Stem Cells Dev 21:2642–2655CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Cellular Reprogramming Laboratory, Department of Biotechnology, School of BiotechnologyInternational University, Vietnam National UniversityHo Chi Minh CityVietnam

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