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Optical microscopy and autofluorescence recognition of Toxoplasma gondii and Cryptosporidium parvum oocysts

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VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016

Part of the book series: IFMBE Proceedings ((IFMBE,volume 60))

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Abstract

OocystsToxoplasma gondii and Cryptosporidium parvum were visualized using conventional optical microscopy (Olympus microscope CX31), allowing recognize their main morphological characteristics. In addition, its ability to autofluorescence when were exposed to certain range of ultraviolet light was confirmed. Cryptosporidium parvum presented autofluorescence under the FITC filter (excitation 450-490 nm and emission 515 nm) and Toxoplasma gondii under (excitation 330-380 nm and emission 397 nm) UV filter. The above was done to develop an autofluorescence experimental protocol for recognition of C. parvum and T. gondii oocysts in samples of raw cow’s milk.

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Authors and Affiliations

  1. Bioingenería, Universidad El Bosque, Bogotá, Colombia

    Alejandra Alba, Laura Baker, Graciela Juez & Andrés Ramírez

Authors
  1. Alejandra Alba
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  2. Laura Baker
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  3. Graciela Juez
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  4. Andrés Ramírez
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Corresponding author

Correspondence to Alejandra Alba .

Editor information

Editors and Affiliations

  1. Asociación Colombiana de Bioingeniería y Electrónica Médica - ABIOIN, Bucamaranga, Colombia

    Isnardo Torres

  2. Universidad Pontificia Bolivariana - UPB, Centro de Bioingeniería, Medellín, Colombia

    John Bustamante

  3. Universidad Industrial de Santander, Escuela de Ing. Eléctrica, Electrónica, Bucaramanga, Colombia

    Daniel A. Sierra

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© 2017 Springer Nature Singapore Pte Ltd.

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Alba, A., Baker, L., Juez, G., Ramírez, A. (2017). Optical microscopy and autofluorescence recognition of Toxoplasma gondii and Cryptosporidium parvum oocysts. In: Torres, I., Bustamante, J., Sierra, D. (eds) VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016. IFMBE Proceedings, vol 60. Springer, Singapore. https://doi.org/10.1007/978-981-10-4086-3_71

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  • DOI: https://doi.org/10.1007/978-981-10-4086-3_71

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-4085-6

  • Online ISBN: 978-981-10-4086-3

  • eBook Packages: EngineeringEngineering (R0)

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