Microbial Repositories in Bioprospecting

  • Gayathri Valsala
  • Shiburaj SugathanEmail author


Microbial diversity is an integral part of ecosystem and is a ticket to each country’s prosperity because it enables the development of a framework for long-term conservation and facilitates targeted bioprospecting initiatives that can potentially catalyse industrial innovation. Microbial culture banks, also called culture collections, are the key repositories of biodiversity. Microbial cultures from the collections are used by scientists to make sure that the materials they use are authentic. This is particularly important in research leading to peer-reviewed publications and patent. Reference strains are also of paramount importance for clinical diagnostic testing, food, water and environmental microbiology testing and validation studies. There are 708 microbial culture collections registered under World Federation of Culture Collections (WFCC). This chapter deals with the current international status of microbial culture collections, their functions and services they provide to meet the current and future demands relevant to biodiversity conservation and biotechnology.


Microbial diversity Bioprospecting Microbial Repository Culture Collection WFCC 


  1. Arora DK, Saikia R, Dwievdi R, Smith D (2005) Current status, strategy and future prospects of microbial resource collections. Curr Sci 89(3):488–495Google Scholar
  2. Bérdy J (2005) Bioactive microbial metabolites. J Antibiot 58(1):1–26CrossRefPubMedGoogle Scholar
  3. Braga PAC, Tata A, dos Santos VG, Barreiro JR, Schwab NV, dos Santos MV, Eberlin MN, Ferreira CR (2013) Bacterial identification: from the agar plate to the mass spectrometer. RSC Adv 3:994–1008CrossRefGoogle Scholar
  4. Broughton R, Buddie AG, Smith D, Ryan MJ (2012) The effect of cryopreservation on genomic stability in strains of the fungus Trichoderma. CryoLetters 33:299–306PubMedGoogle Scholar
  5. Çaktu K, Turkoglu EA (2011) Microbial culture collections: The essential resources for life. Gazi Univ J Sci 24(2):175–180Google Scholar
  6. Chapman AD (2009) Numbers of living species in Australia and the world, 2nd edn. Australian Government, Department of the Environment, Water, Heritage and the Arts, CanberraGoogle Scholar
  7. Crespi RS (1985) Microbiological inventions and the patent law-the international dimension. Biotechnol Genet Eng Rev 3:1–37CrossRefPubMedGoogle Scholar
  8. Davison A, Brabandere J, Smith D (1998) Microbes, collections and the MOSAICC approach. Microbiol Aust 19(1):36–37Google Scholar
  9. Dumont F, Marechal PA, Gervais P (2004) Cell size and water permeability as determining factors for cell viability after freezing at different cooling rates. Appl Environ Microbiol 70:268–272CrossRefPubMedPubMedCentralGoogle Scholar
  10. Fuller BJ (2004) Cryoprotectants: the essential antifreezes to protect life in the frozen state. CryoLetters 25:375–388PubMedGoogle Scholar
  11. Gottel NR, Castro HF, Kerley M, Yang Z, Pelletier DA, Podar M, Karpinets T, Uberbacher E, Tuskan GA, Vilgalys R, Doktycz MJ, Schadt CW (2011) Distinct microbial communities within the endosphere and rhizosphere of Populus deltoides roots across contrasting soil types. Appl Environ Microbiol 77(17):5934–5944CrossRefPubMedPubMedCentralGoogle Scholar
  12. Hawksworth DL (1985) Fungus culture collections as a biotechnological resource. Biotechnol Genet Eng Rev 3:417–453CrossRefPubMedGoogle Scholar
  13. Komagata K (1998) Microbial diversity and the role of culture collections. Pure Appl Chem 70(11):2110–2118Google Scholar
  14. Locey KJ, Lennon JT (2016) Scaling laws predict global microbial diversity. Proc Natl Acad Sci 113(21):5970–5975CrossRefPubMedPubMedCentralGoogle Scholar
  15. Mahilum-Tapay LM (2009) The importance of microbial culture collection and Gene Banks in biotechnology. In: Doelle HW, Rokem JS, Berovic M (eds) Biotechnology. Encyclopaedia of Life Support Systems (EOLSS). Eolss Publishers, OxfordGoogle Scholar
  16. Malik KA, Claus D (1987) Bacterial culture collections: their importance to biotechnology and microbiology. Biotechnol Genet Eng Rev 5:137–197CrossRefPubMedGoogle Scholar
  17. Morgan CA, Herman N, White PA, Vesey G (2006) Preservation of micro-organisms by drying; a review. J Microbiol Methods 66:183–193CrossRefPubMedGoogle Scholar
  18. Muller J, Day JG, Harding K, Hepperle D, Lorenz M, Friedl T (2007) Assessing genetic stability of a range of terrestrial microalgae after cryopreservation using amplified fragment length polymorphism (AFLP). Am J Bot 94:799–808CrossRefPubMedGoogle Scholar
  19. Prakash O, Nimonkar Y, Shouche YS (2013) Practice and prospects of microbial preservation. FEMS Microbiol Lett 339:1–9CrossRefPubMedGoogle Scholar
  20. Ragimbeau C, Schneider F, Losch S, Even J, Mossong J (2008) Multilocus sequence typing, pulsed-field gel electrophoresis, and fla short variable region typing of clonal complexes of Campylobacter jejuni strains of human, bovine, and poultry origins in Luxembourg. Appl Environ Microbiol 74:7715–7722CrossRefPubMedPubMedCentralGoogle Scholar
  21. Sharma A, Shouche Y (2014) Microbial Culture Collection (MCC) and International Depositary Authority (IDA) at National Centre for Cell Science, Pune. Indian J Microbiol 54(2):129–133CrossRefPubMedPubMedCentralGoogle Scholar
  22. Singh P, Dash SS (2014) Plant discoveries 2013 – New genera, species and new records. Botanical Survey of India, KolkataGoogle Scholar
  23. Singh S, Nain L (2014) Microorganisms in the Conversion of Agricultural Wastes to Compost. Proc Indian Natl Sci Acad (Special Section) 80(2):473–481CrossRefGoogle Scholar
  24. Smith D, Ryan MJ, Stackebrandt E (2008) The ex situ conservation of microorganisms: aiming at a certified quality management. In: Doelle HW, EJ DS (eds) Biotechnology. Encyclopaedia of life support systems. Eolss Publishers, OxfordGoogle Scholar
  25. Smith D, McCluskey K, Stackebrandt E (2014) Investment into the future of microbial resources: culture collection funding models and BRC business plans for biological resource centres. Springer Plus 3:81CrossRefPubMedPubMedCentralGoogle Scholar
  26. Staley JT, Castenholz RW, Colwell RR, Holt JG, Kane MD, Pace NR, Saylers AA, Tiedje JM (1997) The microbial world: foundation of the biosphere. American Academy of Microbiology, Washington, DC, p 32Google Scholar
  27. Tsui CKM, Woodhall J, Chen W, Chen W, Levesque CA, Lau A, Schoen CD, Baschien C, Najafzadeh MJ, de Hoog GS (2011) Molecular techniques for pathogen identification and fungus detection in the environment. IMA Fungus 2(2):177–189CrossRefPubMedPubMedCentralGoogle Scholar
  28. Uruburu F (2003) History and services of culture collections. Int Microbiol 6:101–103CrossRefPubMedGoogle Scholar
  29. Uzunova-Doneva T, Donev T (2005) Anabiosis and conservation of microorganisms. J Cult Collect 4(1):17–28Google Scholar
  30. Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A 87:4576–4579CrossRefPubMedPubMedCentralGoogle Scholar
  31. Yandigeri M, Mesapogu S, Yadav A, Arora DK (2010) Microbial culture banks: custodian of real natural wealth. Paper presented at national conference on biodiversity, development and poverty alleviation, Uttar Pradesh State Biodiversity Board, 22 May 2010Google Scholar

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© Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.Division of MicrobiologyJawaharlal Nehru Tropical Botanic Garden and Research InstituteThiruvananthapuramIndia

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