Abstract
Tissue engineering and regenerative medicine (TERM) is an interdisciplinary field encompassing many disciplines, including engineering, medicine, and science. The field has gained an enormous attention due to its potential to replace damaged human tissues and organs and restore normal function [1–3]. Although the early concept of tissue engineering was developed based on cell culture techniques, recent advances in the field combine multiple innovative technologies to accelerate the translation of clinical therapies. In fact, a number of TERM technologies have advanced to human clinical trials and commercialized [4, 5]. In this chapter, we review the current developments and recent progresses made in the field of TERM and discuss the most relevant challenges and future perspectives in the translation of TERM research.
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References
Kemp P. History of regenerative medicine: looking backwards to move forwards. Regen Med. 2006;1(5):653–69.
Vacanti CA. The history of tissue engineering. J Cell Mol Med. 2006;10(3):569–76.
Mao AS, Mooney DJ. Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci U S A. 2015;112(47):14452–9.
Fisher MB, Mauck RL. Tissue engineering and regenerative medicine: recent innovations and the transition to translation. Tissue Eng Part B Rev. 2013;19(1):1–13.
Gemmiti C. Tissue engineering/regenerative medicine ventures should invest early in market research to understand the future market's needs. Tissue Eng Part B-Re. 2013;19(2):97–8.
Vacanti JP. Tissue engineering: from bench to bedside via commercialization. Surgery. 2008;143(2):181–3.
Martin I, Smith T, Wendt D. Bioreactor-based roadmap for the translation of tissue engineering strategies into clinical products. Trends Biotechnol. 2009;27(9):495–502.
Caione P, Boldrini R, Salerno A, Nappo SG. Bladder augmentation using acellular collagen biomatrix: a pilot experience in exstrophic patients. Pediatr Surg Int. 2012;28(4):421–8.
de Jonge PK, Simaioforidis V, Geutjes PJ, Oosterwijk E, Feitz WF. Recent advances in ureteral tissue engineering. Curr Urol Rep. 2015;16(1):465.
Horst M, Madduri S, Gobet R, Sulser T, Milleret V, Hall H, et al. Engineering functional bladder tissues. J Tissue Eng Regen Med. 2013;7(7):515–22.
de Kemp V, de Graaf P, Fledderus JO, Ruud Bosch JL, de Kort LM. Tissue engineering for human urethral reconstruction: systematic review of recent literature. PLoS One. 2015;10(2):e0118653.
Atala A, Bauer SB, Soker S, Yoo JJ, Retik AB. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet. 2006;367(9518):1241–6.
Carr LK, Robert M, Kultgen PL, Herschorn S, Birch C, Murphy M, et al. Autologous muscle derived cell therapy for stress urinary incontinence: a prospective, dose ranging study. J Urol. 2013;189(2):595–601.
Orabi H, AbouShwareb T, Zhang Y, Yoo JJ, Atala A. Cell-seeded tubularized scaffolds for reconstruction of long urethral defects: a preclinical study. Eur Urol. 2013;63(3):531–8.
Schnabel M, Marlovits S, Eckhoff G, Fichtel I, Gotzen L, Vecsei V, et al. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthr Cartil. 2002;10(1):62–70.
Wagers AJ. The stem cell niche in regenerative medicine. Cell Stem Cell. 2012;10(4):362–9.
Bianco P, Cao X, Frenette PS, Mao JJ, Robey PG, Simmons PJ, et al. The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine. Nat Med. 2013;19(1):35–42.
Mahla RS. Stem cells applications in regenerative medicine and disease therapeutics. Int J Cell Biol. 2016;2016:6940283.
Kim JH, Song YS. Current status of stem cell therapy in urology. Korean J Urol. 2015;56(6):409–11.
Fu Q, Cao YL. Tissue engineering and stem cell application of urethroplasty: from bench to bedside. Urology. 2012;79(2):246–53.
King NM, Perrin J. Ethical issues in stem cell research and therapy. Stem Cell Res Ther. 2014;5(4):85.
Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-Hedges R, Ma H, et al. Human embryonic stem cells derived by somatic cell nuclear transfer. Cell. 2013;153(6):1228–38.
Liu G, Pareta RA, Wu R, Shi Y, Zhou X, Liu H, et al. Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. Biomaterials. 2013;34(4):1311–26.
Kobolak J, Dinnyes A, Memic A, Khademhosseini A, Mobasheri A. Mesenchymal stem cells: identification, phenotypic characterization, biological properties and potential for regenerative medicine through biomaterial micro-engineering of their niche. Methods. 2016;99:62–8.
Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211–28.
Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 2004;6(1):7–14.
Rhie JW. Adipose-derived stem cells: characterization and clinical application. J Korean Med Assoc. 2012;55(8):757–69.
Qin D, Long T, Deng J, Zhang Y. Urine-derived stem cells for potential use in bladder repair. Stem Cell Res Ther. 2014;5(3):69.
Dziadosz M, Basch RS, Young BK. Human amniotic fluid: a source of stem cells for possible therapeutic use. Am J Obstet Gynecol. 2016;214(3):321–7.
De Coppi P, Bartsch G Jr, Siddiqui MM, Xu T, Santos CC, Perin L, et al. Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol. 2007;25(1):100–6.
Murphy SV, Atala A. Amniotic fluid and placental membranes: unexpected sources of highly multipotent cells. Semin Reprod Med. 2013;31(1):62–8.
Chun SY, Kwon JB, Chae SY, Lee JK, Bae JS, Kim BS, et al. Combined injection of three different lineages of early-differentiating human amniotic fluid-derived cells restores urethral sphincter function in urinary incontinence. BJU Int. 2014;114(5):770–83.
Wilson KD, Wu JC. Induced pluripotent stem cells. JAMA. 2015;313(16):1613–4.
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126(4):663–76.
Shi Y. Induced pluripotent stem cells, new tools for drug discovery and new hope for stem cell therapies. Curr Mol Pharmacol. 2009;2(1):15–8.
Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, et al. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the predilection of breast cancer patients to doxorubicin-induced cardiotoxicity. Nat Med. 2016;22(5):547–56.
Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. 2007;318(5858):1917–20.
Wang Z, Wen Y, Li YH, Wei Y, Green M, Wani P, et al. Smooth muscle precursor cells derived from human pluripotent stem cells for treatment of stress urinary incontinence. Stem Cells Dev. 2016;25(6):453–61.
Lister R, Pelizzola M, Kida YS, Hawkins RD, Nery JR, Hon G, et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells. Nature. 2011;471(7336):68–73.
Das AK, Pal R. Induced pluripotent stem cells (iPSCs): the emergence of a new champion in stem cell technology-driven biomedical applications. J Tissue Eng Regen Med. 2010;4:413–21.
O'Malley J, Woltjen K, Kaji K. New strategies to generate induced pluripotent stem cells. Curr Opin Biotechnol. 2009;20(5):516–21.
Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Direct conversion of fibroblasts to functional neurons by defined factors. Nature. 2010;463:1035–41.
Zhang Y, McNeill E, Tian H, Soker S, Andersson KE, Yoo JJ, et al. Urine derived cells are a potential source for urological tissue reconstruction. J Urol. 2008;180(5):2226–33.
Keane TJ, Badylak SF. Biomaterials for tissue engineering applications. Semin Pediatr Surg. 2014;23(3):112–8.
Dawson E, Mapili G, Erickson K, Taqvi S, Roy K. Biomaterials for stem cell differentiation. Adv Drug Deliv Rev. 2008;60(2):215–28.
Hunt JA, Chen R, van Veen T, Bryan N. Hydrogels for tissue engineering and regenerative medicine. J Mater Chem B. 2014;2(33):5319–38.
El-Sherbiny IM, Yacoub MH. Hydrogel scaffolds for tissue engineering: Progress and challenges. Global cardiology science & practice. 2013;2013(3):316–42.
Koutsopoulos S. Self-assembling peptide nanofiber hydrogels in tissue engineering and regenerative medicine: progress, design guidelines, and applications. J Biomed Mater Res A. 2016;104(4):1002–16.
Mieszawska AJ, Kaplan DL. Smart biomaterials – regulating cell behavior through signaling molecules. BMC Biol. 2010;8:59.
Zambuzzi WF, Coelho PG, Alves GG, Granjeiro JM. Intracellular signal transduction as a factor in the development of "smart" biomaterials for bone tissue engineering. Biotechnol Bioeng. 2011;108(6):1246–50.
Lee SJ, Van Dyke M, Atala A, Yoo JJ. Host cell mobilization for in situ tissue regeneration. Rejuvenation Res. 2008;11(4):747–56.
Kim JH, Jung Y, Kim BS, Kim SH. Stem cell recruitment and angiogenesis of neuropeptide substance P coupled with self-assembling peptide nanofiber in a mouse hind limb ischemia model. Biomaterials. 2013;34(6):1657–68.
Ko IK, Lee SJ, Atala A, Yoo JJ. In situ tissue regeneration through host stem cell recruitment. Exp Mol Med. 2013;45:e57.
Badylak SF, Taylor D, Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng. 2011;13:27–53.
Schaefer M, Kaiser A, Stehr M, Beyer HJ. Bladder augmentation with small intestinal submucosa leads to unsatisfactory long-term results. J Pediatr Urol. 2013;9(6 Pt A):878–83.
Zhang F, Liao L. Tissue engineered cystoplasty augmentation for treatment of neurogenic bladder using small intestinal submucosa: an exploratory study. J Urol. 2014;192(2):544–50.
Joseph DB, Borer JG, De Filippo RE, Hodges SJ, McLorie GA. Autologous cell seeded biodegradable scaffold for augmentation cystoplasty: phase II study in children and adolescents with spina bifida. J Urol. 2014;191(5):1389–95.
Uygun BE, Soto-Gutierrez A, Yagi H, Izamis ML, Guzzardi MA, Shulman C, et al. Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med. 2010;16(7):814–20.
Guyette JP, Gilpin SE, Charest JM, Tapias LF, Ren X, Ott HC. Perfusion decellularization of whole organs. Nat Protoc. 2014;9(6):1451–68.
Kitahara H, Yagi H, Tajima K, Okamoto K, Yoshitake A, Aeba R, et al. Heterotopic transplantation of a decellularized and recellularized whole porcine heart. Interact Cardiovasc Thorac Surg. 2016;22(5):571–9.
Folkman J, Hochberg M. Self-regulation of growth in three dimensions. J Exp Med. 1973;138(4):745–53.
Helmlinger G, Yuan F, Dellian M, Jain RK. Interstitial pH and pO2 gradients in solid tumors in vivo: high-resolution measurements reveal a lack of correlation. Nat Med. 1997;3(2):177–82.
Novosel EC, Kleinhans C, Kluger PJ. Vascularization is the key challenge in tissue engineering. Adv Drug Deliv Rev. 2011;63(4–5):300–11.
Imbeault A, Bernard G, Rousseau A, Morissette A, Chabaud S, Bouhout S, et al. An endothelialized urothelial cell-seeded tubular graft for urethral replacement. Can Urol Assoc J. 2013;7(1–2):E4–9.
Mercado-Pagan AE, Stahl AM, Shanjani Y, Yang Y. Vascularization in bone tissue engineering constructs. Ann Biomed Eng. 2015;43(3):718–29.
Sun X, Altalhi W, Nunes SS. Vascularization strategies of engineered tissues and their application in cardiac regeneration. Adv Drug Deliv Rev. 2016;96:183–94.
Qazi TH, Mooney DJ, Pumberger M, Geissler S, Duda GN. Biomaterials based strategies for skeletal muscle tissue engineering: existing technologies and future trends. Biomaterials. 2015;53:502–21.
Frueh FS, Menger MD, Lindenblatt N, Giovanoli P, Laschke MW. Current and emerging vascularization strategies in skin tissue engineering. Crit Rev Biotechnol. 2017;37:613–25.
Stabler CT, Caires LC Jr, Mondrinos MJ, Marcinkiewicz C, Lazarovici P, Wolfson MR, et al. Enhanced re-endothelialization of decellularized rat lungs. Tissue Eng Part C Methods. 2016;22(5):439–50.
Rouwkema J, Khademhosseini A. Vascularization and angiogenesis in tissue engineering: beyond creating static networks. Trends Biotechnol. 2016;34(9):733–45.
Jia W, Tang H, Wu J, Hou X, Chen B, Chen W, et al. Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model. Biomaterials. 2015;69:45–55.
Jiang X, Xiong Q, Xu G, Lin H, Fang X, Cui D, et al. VEGF-loaded nanoparticle-modified BAMAs enhance angiogenesis and inhibit graft shrinkage in tissue-engineered bladder. Ann Biomed Eng. 2015;43(10):2577–86.
Shepherd BR, Enis DR, Wang F, Suarez Y, Pober JS, Schechner JS. Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothelial cells. FASEB J. 2006;20(10):1739–41.
Levenberg S, Rouwkema J, Macdonald M, Garfein ES, Kohane DS, Darland DC, et al. Engineering vascularized skeletal muscle tissue. Nat Biotechnol. 2005;23(7):879–84.
Kaully T, Kaufman-Francis K, Lesman A, Levenberg S. Vascularization-the conduit to viable engineered tissues. Tissue Eng Part B Rev. 2009;15(2):159–69.
Kim JH, Jung Y, Kim SH, Sun K, Choi J, Kim HC, et al. The enhancement of mature vessel formation and cardiac function in infarcted hearts using dual growth factor delivery with self-assembling peptides. Biomaterials. 2011;32(26):6080–8.
Utzinger U, Baggett B, Weiss JA, Hoying JB, Edgar LT. Large-scale time series microscopy of neovessel growth during angiogenesis. Angiogenesis. 2015;18(3):219–32.
Laschke MW, Menger MD. Prevascularization in tissue engineering: current concepts and future directions. Biotechnol Adv. 2016;34(2):112–21.
Baranski JD, Chaturvedi RR, Stevens KR, Eyckmans J, Carvalho B, Solorzano RD, et al. Geometric control of vascular networks to enhance engineered tissue integration and function. Proc Natl Acad Sci U S A. 2013;110(19):7586–91.
Kolesky DB, Homan KA, Skylar-Scott MA, Lewis JA. Three-dimensional bioprinting of thick vascularized tissues. Proc Natl Acad Sci U S A. 2016;113(12):3179–84.
Lee VK, Kim DY, Ngo H, Lee Y, Seo L, Yoo SS, et al. Creating perfused functional vascular channels using 3D bio-printing technology. Biomaterials. 2014;35(28):8092–102.
Tuzlakoglu K, Reis RL. Biodegradable polymeric fiber structures in tissue engineering. Tissue Eng Part B Rev. 2009;15(1):17–27.
Quint C, Kondo Y, Manson RJ, Lawson JH, Dardik A, Niklason LE. Decellularized tissue-engineered blood vessel as an arterial conduit. Proc Natl Acad Sci U S A. 2011;108(22):9214–9.
Plunkett N, O’Brien FJ. Bioreactors in tissue engineering. Technol Health Care. 2011;19(1):55–69.
Hansmann J, Groeber F, Kahlig A, Kleinhans C, Walles H. Bioreactors in tissue engineering – principles, applications and commercial constraints. Biotechnol J. 2013;8(3):298–307.
Gardel LS, Serra LA, Reis RL, Gomes ME. Use of perfusion bioreactors and large animal models for long bone tissue engineering. Tissue Eng Part B-Re. 2014;20(2):126–46.
Tocchio A, Tamplenizza M, Martello F, Gerges I, Rossi E, Argentiere S, et al. Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor. Biomaterials. 2015;45:124–31.
Amrollahi P, Tayebi L. Bioreactors for heart valve tissue engineering: a review. J Chem Technol Biot. 2016;91(4):847–56.
Murphy SV, Atala A. Organ engineering–combining stem cells, biomaterials, and bioreactors to produce bioengineered organs for transplantation. BioEssays. 2013;35(3):163–72.
Davis NF, Mooney R, Piterina AV, Callanan A, McGuire BB, Flood HD, et al. Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes. Urology. 2011;78(4):954–60.
Heher P, Maleiner B, Pruller J, Teuschl AH, Kollmitzer J, Monforte X, et al. A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain. Acta Biomater. 2015;24:251–65.
Massai D, Cerino G, Gallo D, Pennella F, Deriu MA, Rodriguez A, et al. Bioreactors as engineering support to treat cardiac muscle and vascular disease. J Healthcare Eng. 2013;4(3):329–70.
Ahn H, Ju YM, Takahashi H, Williams DF, Yoo JJ, Lee SJ, et al. Engineered small diameter vascular grafts by combining cell sheet engineering and electrospinning technology. Acta Biomater. 2015;16:14–22.
Depprich R, Handschel J, Wiesmann HP, Jasche-Meyer J, Meyer U. Use of bioreactors in maxillofacial tissue engineering. Br J Oral Maxillofac Surg. 2008;46(5):349–54.
Billiet T, Vandenhaute M, Schelfhout J, Van Vlierberghe S, Dubruel P. A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. Biomaterials. 2012;33(26):6020–41.
Shafiee A, Atala A. Printing Technologies for Medical Applications. Trends Mol Med. 2016;22(3):254–65.
Soliman Y, Feibus AH, Baum N. 3D printing and its urologic applications. Rev Urol. 2015;17(1):20–4.
Atala A, Kasper FK, Mikos AG. Engineering complex tissues. Sci Transl Med. 2012;4(160):160rv12.
Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol. 2014;32(8):773–85.
Ozbolat IT, Peng W, Ozbolat V. Application areas of 3D bioprinting. Drug Discov Today. 2016;21(8):1257–71.
Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol. 2016;34(3):312–9.
Nam SY, Ricles LM, Suggs LJ, Emelianov SY. Imaging strategies for tissue engineering applications. Tissue Eng Part B Rev. 2015;21(1):88–102.
Appel AA, Anastasio MA, Larson JC, Brey EM. Imaging challenges in biomaterials and tissue engineering. Biomaterials. 2013;34(28):6615–30.
Huang X, Zhang F, Wang Y, Sun X, Choi KY, Liu D, et al. Design considerations of iron-based nanoclusters for noninvasive tracking of mesenchymal stem cell homing. ACS Nano. 2014;8(5):4403–14.
Hatzikirou H, Deutsch A. Cellular automata as microscopic models of cell migration in heterogeneous environments. Curr Top Dev Biol. 2008;81:401–34.
Kumar SP, Feidler JC. BioSPICE: a computational infrastructure for integrative biology. OMICS. 2003;7(3):225.
Hunt CJ. Cryopreservation of human stem cells for clinical application: a review. Transfusion Med Hemother. 2011;38(2):107–23.
Wang Z, Qin TW. Review: vitreous cryopreservation of tissue-engineered compositions for tissue repair. J Med Biol Eng. 2013;33(2):125–31.
Donnez J, Dolmans MM, Pellicer A, Diaz-Garcia C, Sanchez Serrano M, Schmidt KT, et al. Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation. Fertil Steril. 2013;99(6):1503–13.
Sadri-Ardekani H, McLean TW, Kogan S, Sirintrapun J, Crowell K, Yousif MQ, et al. Experimental testicular tissue banking to generate spermatogenesis in the future: a multidisciplinary team approach. Methods. 2016;99:120–7.
Raya-Rivera A, Esquiliano DR, Yoo JJ, Lopez-Bayghen E, Soker S, Atala A. Tissue-engineered autologous urethras for patients who need reconstruction: an observational study. Lancet. 2011;377(9772):1175–82.
Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med. 2010;363(2):147–55.
Makkar RR, Smith RR, Cheng K, Malliaras K, Thomson LE, Berman D, et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet. 2012;379(9819):895–904.
Bolli R, Chugh AR, D'Amario D, Loughran JH, Stoddard MF, Ikram S, et al. Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trial. Lancet. 2011;378(9806):1847–57.
Dimmeler S, Ding S, Rando TA, Trounson A. Translational strategies and challenges in regenerative medicine. Nat Med. 2014;20(8):814–21.
Badylak SF, Weiss DJ, Caplan A, Macchiarini P. Engineered whole organs and complex tissues. Lancet. 2012;379(9819):943–52.
Sicari BM, Rubin JP, Dearth CL, Wolf MT, Ambrosio F, Boninger M, et al. An acellular biologic scaffold promotes skeletal muscle formation in mice and humans with volumetric muscle loss. Sci Transl Med. 2014;6(234):234ra58.
Kang SB, Olson JL, Atala A, Yoo JJ. Functional recovery of completely denervated muscle: implications for innervation of tissue-engineered muscle. Tissue Eng Part A. 2012;18(17–18):1912–20.
Ko IK, Lee BK, Lee SJ, Andersson KE, Atala A, Yoo JJ. The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo. Biomaterials. 2013;34(13):3246–55.
Morimoto Y, Kato-Negishi M, Onoe H, Takeuchi S. Three-dimensional neuron-muscle constructs with neuromuscular junctions. Biomaterials. 2013;34(37):9413–9.
Hunsberger J, Harrysson O, Shirwaiker R, Starly B, Wysk R, Cohen P, et al. Manufacturing road map for tissue engineering and regenerative medicine technologies. Stem Cells Transl Med. 2015;4(2):130–5.
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The authors thank Margaret vanSchaayk for editorial assistance with this manuscript.
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Kim, J.H., Yoo, J.J. (2018). Current Developments and Future Perspectives of Tissue Engineering and Regenerative Medicine. In: Kim, B. (eds) Clinical Regenerative Medicine in Urology. Springer, Singapore. https://doi.org/10.1007/978-981-10-2723-9_1
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