Skin Regeneration

  • Xiaowen Zheng
  • Qian Li
  • Lie MaEmail author
  • Changyou Gao


The engineering of skin substitutes and their applications on the regeneration of damaged skin have advanced dramatically in the past decades. However, scientists are still struggling with the generation of full-thickness skin with native structure and completed functions. In this chapter, classified by sources, recent developments of biomaterials for skin regeneration have been summarized. Then the most common formats of the engineering skin substitutes are introduced. The strategies of the biological functionalization in the design of skin substitutes are further summarized. Some important challenges in the field of skin substitutes such as angiogenesis, scarring, and appendages loss are particularly focused on. Finally, a brief conclusion and some perspectives are given in terms of the future trend of biomaterials for skin regeneration.


Skin Scaffold Bio-functionalization In situ regeneration Regenerative medicine 



We acknowledge financial support by the Key Science Technology Innovation Team of Zhejiang Province (2013TD02), the Natural Science Foundation of China (51322302, 20934003) and the National Key Research Program of China (2016YFC1101001).


  1. 1.
    MacNeil S. Progress and opportunities for tissue-engineered skin. Nature. 2007;445:874–80.PubMedCrossRefGoogle Scholar
  2. 2.
    Tabata Y. Biomaterial technology for tissue engineering applications. J R Soc Interface. 2009;6(35, Suppl 3):S311–24.PubMedPubMedCentralCrossRefGoogle Scholar
  3. 3.
    Chen FM, Wu LA, Zhang M, et al. Homing of endogenous stem/progenitor cells for in situ, tissue regeneration: promises, strategies, and translational perspectives. Biomaterials. 2011;32(12):3189–209.PubMedCrossRefGoogle Scholar
  4. 4.
    Marston WA. Dermagraft, a bioengineered human dermal equivalent for the treatment of chronic nonhealing diabetic foot ulcer. Expert Rev Med Devices. 2004;1(1):21–31.PubMedCrossRefGoogle Scholar
  5. 5.
    Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-based biomaterials for tissue engineering applications. Materials. 2010;3:1863–87.CrossRefGoogle Scholar
  6. 6.
    Willard JJ, Drexler JW, Das A, et al. Plant-derived human collagen scaffolds for skin tissue engineering. Tissue Eng Part A. 2013;19(13–14):1507–18.PubMedPubMedCentralCrossRefGoogle Scholar
  7. 7.
    Cao H, Chen MM, Liu Y, et al. Fish collagen-based scaffold containing PLGA microspheres for controlled growth factor delivery in skin tissue engineering. Colloids Surf B Biointerfaces. 2015;136:1098–106.PubMedCrossRefGoogle Scholar
  8. 8.
    Rnjak-Kovacina J, Wise SG, Zhe L, et al. Electrospun synthetic human elastin: collagen composite scaffolds for dermal tissue engineering. Acta Biomater. 2012;8(10):3714–22.PubMedCrossRefGoogle Scholar
  9. 9.
    Bellas E, Seiberg M, Garlick J, et al. In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials. Macromol Biosci. 2012;12(12):1627–36.PubMedPubMedCentralCrossRefGoogle Scholar
  10. 10.
    Ma L, Gao C, Mao Z, et al. Thermal dehydration treatment and glutaraldehyde cross-linking to increase the biostability of collagen-chitosan porous scaffolds used as dermal equivalent. J Biomater Sci Polym Ed. 2003;14(8):861–74.PubMedCrossRefGoogle Scholar
  11. 11.
    Ma L, Gao C, Mao Z, et al. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials. 2003;24(26):4833–41.PubMedCrossRefGoogle Scholar
  12. 12.
    Wang X, You C, Hu X, et al. The roles of knitted mesh-reinforced collagen-chitosan hybrid scaffold in the one-step repair of full-thickness skin defects in rats. Acta Biomater. 2013;9(8):7822–32.PubMedCrossRefGoogle Scholar
  13. 13.
    Zhong SP, Zhang YZ, Lim CT. Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010;2:510–25.PubMedCrossRefGoogle Scholar
  14. 14.
    Edmonds M. Apligraf in the treatment of neuropathic diabetic foot ulcers. Int J Lower Extrem Wounds. 2009;8(1):11–8.CrossRefGoogle Scholar
  15. 15.
    Montembault A, Viton C, Domard A. Physico-chemical studies of the gelation of chitosan in a hydroalcoholic medium. Biomaterials. 2005;26(8):933–43.PubMedCrossRefGoogle Scholar
  16. 16.
    Ribeiro MP, Ana E, Daniela S, et al. Development of a new chitosan hydrogel for wound dressing. Wound Repair Regen. 2009;17(6):817–24.PubMedCrossRefGoogle Scholar
  17. 17.
    Adekogbe I, Ghanem A. Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering. Biomaterials. 2005;26(35):7241–50.PubMedCrossRefGoogle Scholar
  18. 18.
    Hong JP, Kim YW, Lee SK, et al. The effect of continuous release of recombinant human epidermal growth factor (rh-EGF) in chitosan film on full thickness excisional porcine wounds. Ann Plast Surg. 2008;61(4):457–62.PubMedCrossRefGoogle Scholar
  19. 19.
    Mizuno K, Yamamura K, Yano K, et al. Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice. J Biomed Mater Res A. 2003;64(1):177–81.PubMedCrossRefGoogle Scholar
  20. 20.
    Abdelgawad AM, Hudson SM, Rojas OJ. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydr Polym. 2014;100(100):166–78.PubMedCrossRefGoogle Scholar
  21. 21.
    Tchemtchoua VT, Atanasova G, Aqil A, et al. Development of a chitosan nanofibrillar scaffold for skin repair and regeneration. Biomacromolecules. 2011;12(9):3194–204.PubMedCrossRefGoogle Scholar
  22. 22.
    Kiyozumi T, Kanatani Y, Ishihara M, Saitoh D, Shimizu J, Yura H, et al. Medium (DMEM/F12)-containing chitosan hydrogel as adhesive and dressing in autologous skin grafts and accelerator in the healing process. J Biomed Mater Res B Appl Biomater. 2006;79:129–36.PubMedCrossRefGoogle Scholar
  23. 23.
    Kiyozumi T, Kanatani Y, Ishihara M, et al. The effect of chitosan hydrogel containing DMEM/F12 medium on full-thickness skin defects after deep dermal burn. Burns. 2007;33(5):642–8.PubMedCrossRefGoogle Scholar
  24. 24.
    Shevchenko RV, Eeman M, Rowshanravan B, et al. The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. Acta Biomater. 2014;10(7):3156–66.PubMedCrossRefGoogle Scholar
  25. 25.
    Monteiro IP, Shukla A, Marques AP, et al. Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering. J Biomed Mater Res A. 2015;103(1):330–40.PubMedCrossRefGoogle Scholar
  26. 26.
    Losi P, Briganti E, Errico C, et al. Fibrin-based scaffold incorporating VEGF- and bFGF-loaded nanoparticles stimulates wound healing in diabetic mice. Acta Biomater. 2013;9(8):7814–21.PubMedCrossRefGoogle Scholar
  27. 27.
    Rezwan K, Chen QZ, Blaker JJ, et al. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials. 2006;27(18):3413–31.PubMedCrossRefGoogle Scholar
  28. 28.
    Amani H, Dougherty WR, Blome S. Use of Transcyte® and dermabrasion to treat burns reduces length of stay in burns of all size and etiology. Burns J Int Soc Burn Injuries. 2006;32(7):828–32.CrossRefGoogle Scholar
  29. 29.
    Kumbar SG, Nukavarapu SP, James R, et al. Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials. 2008;29(30):4100–7.PubMedPubMedCentralCrossRefGoogle Scholar
  30. 30.
    Cui W, Zhu X, Yang Y, et al. Evaluation of electrospun fibrous scaffolds of poly(dl -lactide) and poly(ethylene glycol) for skin tissue engineering. Mater Sci Eng C. 2009;29(6):1869–76.CrossRefGoogle Scholar
  31. 31.
    Chen G, Sato T, Ohgushi H, et al. Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh. Biomaterials. 2005;26(15):2559–66.PubMedCrossRefGoogle Scholar
  32. 32.
    Venugopal JR, Zhang Y, Ramakrishna S. In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane. Artif Organs. 2006;30(6):440–6.PubMedCrossRefGoogle Scholar
  33. 33.
    Yang J, Shi G, Bei J, Wang S, Cao Y, Shang Q, et al. Fabrication and surface modification of macroporous poly (L-lactic acid) and poly (L-lactic-co-glycolic acid)(70/30) cell scaffolds for human skin fibroblast cell culture. J Biomed Mater Res. 2002;62:438–46.PubMedCrossRefGoogle Scholar
  34. 34.
    Gautam S, Chou CF, et al. Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Mater Sci Eng C Mater Biol Appl. 2014;34C(1):402–9.CrossRefGoogle Scholar
  35. 35.
    Zhou Y, Yang D, Chen X, et al. Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration. Biomacromolecules. 2008;9(1):349–54.PubMedCrossRefGoogle Scholar
  36. 36.
    Wang HM, Chou YT, Wen ZH, et al. Novel biodegradable porous scaffold applied to skin regeneration. Plos One. 2013;8(6):e56330.PubMedPubMedCentralCrossRefGoogle Scholar
  37. 37.
    Lu H, Oh HH, Kawazoe N, et al. PLLA-collagen and PLLA-gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering. Sci Technol Adv Mater. 2012;13(6):64210–8.CrossRefGoogle Scholar
  38. 38.
    Shi Y, Ma L, Zhou J, et al. Collagen/chitosan-silicone membrane bilayer scaffold as a dermal equivalent. Polym Adv Technol. 2005;16(11–12):789–94.CrossRefGoogle Scholar
  39. 39.
    Rui G, Xu S, Ma L, et al. Enhanced angiogenesis of gene-activated dermal equivalent for treatment of full thickness incisional wounds in a porcine model. Biomaterials. 2010;31(28):7308–20.CrossRefGoogle Scholar
  40. 40.
    Liu X, Liang J, Zhang B, et al. RNAi functionalized collagen-chitosan/silicone membrane bilayer dermal equivalent for full-thickness skin regeneration with inhibited scarring. Biomaterials. 2013;34(8):2038–48.PubMedCrossRefGoogle Scholar
  41. 41.
    Vlierberghe SV, Dubruel P, Schacht E. Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review. Biomacromolecules. 2011;12(5):1387–408.PubMedCrossRefGoogle Scholar
  42. 42.
    Yanchun Liu MD, Cai S, Xiao ZS, et al. Release of basic fibroblast growth factor from a crosslinked glycosaminoglycan hydrogel promotes wound healing. Wound Repair Regen. 2007;15(2):245–51.PubMedCrossRefGoogle Scholar
  43. 43.
    Shepherd J, Sarker P, Rimmer S, et al. Hyperbranched poly(NIPAM) polymers modified with antibiotics for the reduction of bacterial burden in infected human tissue engineered skin. Biomaterials. 2011;32(32):258–67.PubMedCrossRefGoogle Scholar
  44. 44.
    Peattie RA, Nayate AP, Firpo MA, et al. Stimulation of in vivo angiogenesis by cytokine-loaded hyaluronic acid hydrogel implants. Biomaterials. 2004;25(14):2789–98.PubMedCrossRefGoogle Scholar
  45. 45.
    Lee PY, Cobain E, Huard J, et al. Thermosensitive hydrogel PEG-PLGA-PEG enhances engraftment of muscle-derived stem cells and promotes healing in diabetic wound. Mol Ther J Am Soc Gene Ther. 2007;15(6):1189–94.Google Scholar
  46. 46.
    Miguel SP, Ribeiro MP, Brancal H, et al. Thermoresponsive chitosan-agarose hydrogel for skin regeneration. Carbohydr Polym. 2014;111(20):366–73.PubMedCrossRefGoogle Scholar
  47. 47.
    Boucard N, Viton C, Agay D, et al. The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. 2007;28(24):3478–88.PubMedCrossRefGoogle Scholar
  48. 48.
    Murakami K, Aoki H, Nakamura S, et al. Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials. 2010;31(1):83–90.PubMedCrossRefGoogle Scholar
  49. 49.
    Wong VW, Rustad KC, Galvez MG, et al. Engineered pullulan-collagen composite dermal hydrogels improve early cutaneous wound healing. Tissue Eng Part A. 2011;17(5–6):631–44.PubMedCrossRefGoogle Scholar
  50. 50.
    Ribeiro MP, Morgado PI, Miguel SP, et al. Dextran-based hydrogel containing chitosan microparticles loaded with growth factors to be used in wound healing. Mater Sci Eng C. 2013;33(5):2958–66.CrossRefGoogle Scholar
  51. 51.
    Sun G, Zhang X, Shen YI, et al. Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing. Proc Natl Acad Sci U S A. 2011;108(52):20976–81.PubMedPubMedCentralCrossRefGoogle Scholar
  52. 52.
    Richardson TP, Peters MC, Ennett AB, et al. Polymeric system for dual growth factor delivery. Nat Biotechnol. 2001;19(11):1029–34.PubMedCrossRefGoogle Scholar
  53. 53.
    Perets A, Baruch Y, Weisbuch F, et al. Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres. J Biomed Mater Res A. 2003;65a(65):489–97.CrossRefGoogle Scholar
  54. 54.
    Ozeki M, Tabata Y. In vivo promoted growth of mice hair follicles by the controlled release of growth factors. Biomaterials. 2003;24(13):2387–94.PubMedCrossRefGoogle Scholar
  55. 55.
    Mao Z, Ma L, Zhou J, et al. Bioactive thin film of acidic fibroblast growth factor fabricated by layer-by-layer assembly. Bioconjug Chem. 2005;16(5):1316–22.PubMedCrossRefGoogle Scholar
  56. 56.
    Uijtdewilligen PJE, Versteeg EMM, Gilissen C, et al. Towards embryonic-like scaffolds for skin tissue engineering: identification of effector molecules and construction of scaffolds. J Tissue Eng Regen Med. 2013;10(1):E34–44.PubMedCrossRefGoogle Scholar
  57. 57.
    Shea LD, Smiley E, Bonadio J, et al. DNA delivery from polymer matrices for tissue engineering. Nat Biotechnol. 1999;17(6):551–4.PubMedCrossRefGoogle Scholar
  58. 58.
    Hijjawi J, Mogford JE, Chandler LA, et al. Platelet-derived growth factor B, but not fibroblast growth factor 2, plasmid DNA improves survival of ischemic myocutaneous flaps. Arch Surg. 2004;139(2):142–7.PubMedCrossRefGoogle Scholar
  59. 59.
    Putnam D, Doody A. RNA-interference effectors and their delivery. Crit Rev Ther Drug Carrier Syst. 2006;23(2):137–64.PubMedCrossRefGoogle Scholar
  60. 60.
    Laporte LD, Rea JC, Shea LD. Design of modular non-viral gene therapy vectors. Biomaterials. 2006;27(7):947–54.PubMedCrossRefGoogle Scholar
  61. 61.
    Vanden BergFoels WS. In situ tissue regeneration: chemoattractants for endogenous stem cell recruitment. Tissue Eng Part B Rev. 2014;20(1):28–39.CrossRefGoogle Scholar
  62. 62.
    Tang A, Gilchrest BA. Regulation of keratinocyte growth factor gene expression in human skin fibroblasts. J Dermatol Sci. 1996;11(1):41–50.PubMedCrossRefGoogle Scholar
  63. 63.
    Lin ZQ, Kondo T, Ishida Y, et al. Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice. J Leukoc Biol. 2003;73(6):713–21.PubMedCrossRefGoogle Scholar
  64. 64.
    Barrientos S, Stojadinovic O, Golinko MS, et al. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008;16(5):333.CrossRefGoogle Scholar
  65. 65.
    Zhou SB, Wang J, Chiang CA, et al. Mechanical stretch upregulates SDF-1α in skin tissue and induces migration of circulating bone marrow-derived stem cells into the expanded skin. Stem Cells. 2013;31(12):2703–13.PubMedCrossRefGoogle Scholar
  66. 66.
    Nakamura Y, Ishikawa H, Kawai K, et al. Enhanced wound healing by topical administration of mesenchymal stem cells transfected with stromal cell-derived factor-1. Biomaterials. 2013;34(37):9393–400.PubMedCrossRefGoogle Scholar
  67. 67.
    Zhang B, Liu X, Wang C, et al. Chapter 52 – bioengineering skin constructs. In: Stem cell biology and tissue engineering in dental sciences. London: Academic; 2015. p. 703–19.CrossRefGoogle Scholar
  68. 68.
    Black AF, Berthod F, L’Heureux N, et al. In vitro reconstruction of a human capillary-like network in a tissue-engineered skin equivalent. Faseb J. 1998;12(13):1331–40.PubMedGoogle Scholar
  69. 69.
    O’Ceallaigh S, Herrick SE, Bluff JE, et al. Quantification of total and perfused blood vessels in murine skin autografts using a fluorescent double-labeling technique. Plast Reconstruct Surg. 2006;117(1):140–51.CrossRefGoogle Scholar
  70. 70.
    O’Brien FJ, Harley BA, Yannas IV, et al. The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials. 2005;26(4):433–41.PubMedCrossRefGoogle Scholar
  71. 71.
    Pruitt Jr B, Levine NS. Characteristics and uses of biologic dressings and skin substitutes. Arch Surg. 1984;119(3):312–22.PubMedCrossRefGoogle Scholar
  72. 72.
    Böttcher-Haberzeth S, Biedermann T, Klar AS, et al. Tissue engineering of skin: human tonsil-derived mesenchymal cells can function as dermal fibroblasts. Pediatr Surg Int. 2014;30(2):213–22.PubMedCrossRefGoogle Scholar
  73. 73.
    Pieper JS, Wachem PBV, Luyn MJAV, et al. Attachment of glycosaminoglycans to collagenous matrices modulates the tissue response in rats. Biomaterials. 2000;21(16):1689–99.PubMedCrossRefGoogle Scholar
  74. 74.
    Pandit AS, Feldman DS, Caulfield J. In vivo wound healing response to a modified degradable fibrin scaffold. J Biomater Appl. 1998;12(3):222–36.PubMedGoogle Scholar
  75. 75.
    Wissink MJB, Beernink R, Poot AA, et al. Improved endothelialization of vascular grafts by local release of growth factor from heparinized collagen matrices. J Control Release. 2000;64(1–3):103–14.PubMedCrossRefGoogle Scholar
  76. 76.
    Mao Z, Shi H, Rui G, et al. Enhanced angiogenesis of porous collagen scaffolds by incorporation of TMC/DNA complexes encoding vascular endothelial growth factor. Acta Biomater. 2009;5(8):2983–94.PubMedCrossRefGoogle Scholar
  77. 77.
    Guo R, Xu S, Ma L, et al. The healing of full-thickness burns treated by using plasmid DNA encoding VEGF-165 activated collagen-chitosan dermal equivalents. Biomaterials. 2011;32(4):1019–31.PubMedCrossRefGoogle Scholar
  78. 78.
    Costa AMA, Desmoulire A. Mechanisms and factors involved in development of hypertrophic scars. Eur J Plast Surg. 1998;21(1):19–23.CrossRefGoogle Scholar
  79. 79.
    Lappert PW. Scarless fetal skin repair: “unborn patients” and “fetal material”. Plast Reconstr Surg. 1996;98(6):1125.PubMedCrossRefGoogle Scholar
  80. 80.
    Chalmers RL. The evidence for the role of transforming growth factor-beta in the formation of abnormal scarring. Int Wound J. 2011;8(3):218–23.PubMedCrossRefGoogle Scholar
  81. 81.
    Samuels P, Tan AK. Fetal scarless wound healing. J Otolaryngol. 1999;28(5):296–302.PubMedGoogle Scholar
  82. 82.
    Liu W, Chua C, Wu X, et al. Inhibiting scar formation in rat wounds by adenovirus-mediated overexpression of truncated TGF-beta receptor II. Plast Reconstr Surg. 2005;115(3):860–70.PubMedCrossRefGoogle Scholar
  83. 83.
    Monaghan M, Pandit A. RNA interference therapy via functionalized scaffolds. Adv Drug Deliv Rev. 2011;63:197–208.PubMedCrossRefGoogle Scholar
  84. 84.
    Zhong SP, Zhang YZ, Lim CT. Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010;2(5):510–25.PubMedCrossRefGoogle Scholar
  85. 85.
    Yoo BY. Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication. J Dermatol Sci. 2010;60(2):74–83.PubMedCrossRefGoogle Scholar
  86. 86.
    Jin SE, Sung JH. Hair regeneration using adipose-derived stem cells. Histol Histopathol. 2015;31:249–56.PubMedGoogle Scholar
  87. 87.
    Huang S, Xu Y, Wu C, et al. In vitro, constitution and in vivo, implantation of engineered skin constructs with sweat glands. Biomaterials. 2010;31(21):5520–5.PubMedCrossRefGoogle Scholar
  88. 88.
    Huang S, Yao B, Xie J, et al. 3D bioprinted extracellular matrix mimics facilitate directed differentiation of epithelial progenitors for sweat gland regeneration. Acta Biomater. 2015;32:170–7.PubMedCrossRefGoogle Scholar
  89. 89.
    Horsley V, O’Carroll D, Tooze R, et al. Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland. Cell. 2006;126(3):597–609.PubMedPubMedCentralCrossRefGoogle Scholar
  90. 90.
    Chen P, Tao J, Zhu S, et al. Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing. Biomaterials. 2015;39:114–23.PubMedCrossRefGoogle Scholar
  91. 91.
    Ho CJ, Mook LS, In YY, et al. Microenvironmental interaction between hypoxia and endothelial cells controls the migration ability of placenta-derived mesenchymal stem cells via alpha4 integrin and rho signaling. J Cell Biochem. 2015;117(5):1145–57.Google Scholar
  92. 92.
    Shao Z, Zhang X, Pi Y, et al. Polycaprolactone electrospun mesh conjugated with an MSC affinity peptide for MSC homing in vivo. Biomaterials. 2012;33(12):3375–87.PubMedCrossRefGoogle Scholar
  93. 93.
    Wang H, Yan X, Shen L, et al. Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs. Burns Trauma. 2014;2(4):181–6.PubMedPubMedCentralCrossRefGoogle Scholar
  94. 94.
    Man Z, Yin L, Shao Z, et al. The effects of co-delivery of BMSC-affinity peptide and rhTGF-β1 from coaxial electrospun scaffolds on chondrogenic differentiation. Biomaterials. 2014;35:5250–60.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and EngineeringZhejiang UniversityHangzhouChina

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