Abstract
Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Alberttson, P.A.: Partition of Cell Particles and Macromolecules. Separation and Purification of Biomolecules, Cell Organelles, Membranes and Cells in Aqueous Polymer Two-phase Systems and Their Use in Biochemical Analysis and Biotechnology. Wiley-Interscience, New York (1986)
Asenjo, J.A., Andrews, B.A.: Aqueous two-phase systems for protein separation: a perspective. J. Chromatogr. A 1218, 8826–8835 (2011)
Azevedo, A.M., Sommerfeld, S., Mutter, A., Aires-Barros, M.R., et al.: Application of aqueous two-phase systems to antibody purification: a multi stage approach. J. Biotechnol. 139, 306–313 (2009)
Brooks, E., Sharp, K.A., Fischer, D.: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology. Academic Press, New York (1985)
Cardoso, G., Mourao, T., Pereira, F., Freire, M., Fricks, A., Soares, C., Lima, A.: Aqueous two-phase systems based on acetonitrile and carbohydrates and their application to the extraction of vanillin. Sep. Purif. Technol. 104, 106–113 (2013)
Freire, M.G., Louros, C.L.S., Rebelo, L.P.N., Coutinho, J.A.P.: Aqueous biphasic systems composed of a water-stable ionic liquid + carbohydrates and their applications. Green Chem. 13, 1536–1545 (2011)
Gai, Q., Qu, F., Zhang, T., Zhang, Y.: Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins. Talanta 85, 304–309 (2011)
Grolbmann, C., Tintinger, R., Zhu, J., Maurer, G.: Aqueous two-phase systems of poly(ethylene glycol) and dextran—experimental results and modeling of thermodynamic properties. Fluid Phase Equilib. 106, 111–138 (1995)
Guo, W., Ma, J., Wang, Y., Han, J., Li, Y., Song, S.: Liquid–liquid equilibrium of aqueous two-phase systems composed of hydrophilic alcohols (ethanol/2-propanol/1-propanol) and MgSO4/ZnSO4 at (303.15 and 313.15) K and correlation. Thermochim. Acta. 546, 8–15 (2012)
Han, J., Wu, Y., Xiang, Y., Wang, Y., Ma, J., Hu, Y.: Liquid–liquid equilibria of hydrophilic alcohol + sodium hydroxide + water systems: Experimental and correlation. Thermochim. Acta 566, 261–267 (2013)
Kalaivani, S., Srikanth, C.K., Regupathi, I.: Densities and viscosities of binary and ternary mixtures and aqueous two-phase system of poly ethylene glycol 2000 + diammonium hydrogen citrate + water at different temperatures. J. Chem. Eng. Data 57, 2528–2534 (2012)
Liu, Y., Wu, Z., Zhang, Y., Yuan, H.: Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant. Biochem. Eng. J. 69, 93–99 (2012)
Luechau, F., Ling, T.C., Lyddiatt, A.: Primary capture of high molecular weight nucleic acids using aqueous two-phase systems. Sep. Purif. Technol. 66, 202–207 (2009)
Mehrnoush, A., Mustafa, S., Sarker, M.I., Yazi, A.M.M.: Optimization of serine protease purification from mango (Mangifera Indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system. Int. J. Mol. Sci. 13, 3636–3649 (2012) (Rosa PAJ)
Neves, C.M.S.S., Ventura, S.P.M., Freire, M.G., MarruchoI, M., Coutinho, J.A.P.: Evaluation of cation influence on the formation and extraction capability of ionic liquid-based aqueous biphasic systems. J. Phys. Chem. B 113, 5194–5199 (2009)
Pereira, J.F.B., Ventura, S.P.M., Silva, F.A., Shahriari, S., Freire, M.G., Coutinho, J.A.P.: Aqueous biphasic systems composed of ionic liquids and polymers: a platform for the purification of biomolecules. Sep. Purif. Technol. 113, 83–89 (2013)
Pollak, P., Romeder, G., Hagedorn, F., Gelbke, H.P.: Ullmann’s Encyclopedia of Industrial Chemistry. Wiley Online Library, New York (2000)
Ratanapongleka, K.: Recovery of biological products in aqueous two phase systems. Int. J. Chem. Eng. Appl. 1, 191–198 (2010)
Reis, I.A.O., Santos, S.B., Santos, L.A., Oliveira, N., Freire, M.G., Pereira, J.F.B., Ventura, S.P.M., Coutinho, J.A.P., Soares, C.M.F., Lima, A.S.: Increased significance of food wastes: selective recovery of added-value compounds. Food Chem. 135, 2453–2461 (2012)
Rosa, P.A.J., Azevedo, A.M., Sommerfeld, S., Mutter, M., Bäcker, W., et al.: Continuous aqueous two-phase extraction of human antibodies using a packed column. J. Chromatogr. B 880, 148–156 (2012)
Takamuku, T., Tabata, M., Yamaguchi, A., Nishimoto, J., Kumamoto, M., Wakita, H., Yamaguchi, T.: Liquid structure of acetonitrile-water by X-ray diffraction and infrared spectroscopy. J. Phys. Chem. B 102, 8880–8888 (1998)
Taha, M., Teng, H.L., Lee, M.J.: Phase diagrams of acetonitrile or (acetone + water + EPPS) buffer phase separation systems at 298.15 K and quantum chemical modeling. J. Chem. Thermodyn. 54, 134–141 (2012)
Wang, B., Ezejias, T., Feng, H., Blaschek, H.: Sugaring-out: a novel phase separation on extraction system. Chem. Eng. Sci. 63, 25958–26000 (2008)
Wang, Y., Hu, S., Yan, Y., Guan, W.: Liquid-liquid equilibrium of potassium/sodium carbonate + 2-propanol/ethanol + water aqueous two-phase systems and correlation at 298.15 K. CALPHAD: Comput. Coupling Phase Diagrams Thermochem. 33, 726–731 (2009)
Zafarani-Moattar, M., Hosseinpour-Hashemi, V., Banisaeid, S., Beirami, M.: The study of phase behavior of aqueous 1-propanol/2-propanol/2-butanol/2-methyl-2-propanol systems in the presence of disodium tartrate or disodium succinate at T = 298.15 K. Fluid Phase Equilib. 338, 37–45 (2013)
Zhao, X., Xie, X., Yan, Y.: Liquid–liquid equilibrium of aqueous two-phase systems containing poly (propylene glycol) and salt ((NH4)2SO4, MgSO4, KCl, and KAc): experiment and correlation. Thermochim. Acta 516, 46–51 (2011)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media Singapore
About this chapter
Cite this chapter
Dilip, B.S., Iyyaswami, R., Nainegali, B.S., Rashmi, B.S. (2016). Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies. In: B. D., P., Gummadi, S., Vadlani, P. (eds) Biotechnology and Biochemical Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-1920-3_11
Download citation
DOI: https://doi.org/10.1007/978-981-10-1920-3_11
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-1919-7
Online ISBN: 978-981-10-1920-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)