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Plant Tissue Culture: A Journey from Research to Commercialization

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Plant Tissue Culture: Propagation, Conservation and Crop Improvement

Abstract

Tissue culture was a subject of academic interest for a long time. In recent years, it has become a useful tool for agriculture and medicine. It has therefore been a popular area of biological research. Considerable amount of literature has been generated, but it is not commensurate with the results obtained. The continuous and non-organized exploitation has resulted in many medicinal plants becoming rare, and a good number have even become extinct. Therefore, tissue culture has emerged as a science with a vast potential for human welfare ranging from large-scale plant production in horticulture and forestry, human health, plant protection as well as environmental protection. In vitro rejuvenation holds remarkable potentials for the production and superior plant-based medicine. There are mainly four approaches for in vitro germplasm preservation, which may lead to development of a tissue bank; cryopreservation, normally growing and multiplying shoot culture, slow-growth culture and regenerative long-term excised root culture. The main parameter for evaluating the worth of these approaches includes practicability, prolonged retention of regenerative potentiality and the least chances of genetic instability.

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Abbreviations

BA:

6-benzyl adenine

2iP:

2-isopentenyl adenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

Kn:

6-furfurylaminopurine

PGRs:

Plant growth regulators

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthalene acetic acid

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Correspondence to Mohammad Anis .

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Anis, M., Ahmad, N. (2016). Plant Tissue Culture: A Journey from Research to Commercialization. In: Anis, M., Ahmad, N. (eds) Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer, Singapore. https://doi.org/10.1007/978-981-10-1917-3_1

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