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Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review

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Functional Nucleic Acids Detection in Food Safety

Abstract

Detection methods used in food safety can be classified into two categories: amplification and hybridization. Their purpose is to accumulate or to recognize target sequence specifically using a proper reaction buffer in vitro. Polymerase chain reaction (PCR) is a valuable tool for monitoring gene expression, for quantifying foodborne pathogens, and for use in clinical diagnostics. Hybridization methods offer a new frontier in the identification of targets in a simple and high-throughput way. This review mainly describes and compares the basic rules of PCR and hybridization, including their formatting source, characteristics, and applications.

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Acknowledgments

This work is supported by the Ministry of Science and Technology of Beijing (XX2014B069). Many thanks to Chenguang Wang, for his kind help in manuscript conception and preparation.

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  1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China

    Wentao Xu

  2. Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, China

    Wentao Xu

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Xu, W. (2016). Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review. In: Functional Nucleic Acids Detection in Food Safety. Springer, Singapore. https://doi.org/10.1007/978-981-10-1618-9_3

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