Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review

  • Wentao Xu


Detection methods used in food safety can be classified into two categories: amplification and hybridization. Their purpose is to accumulate or to recognize target sequence specifically using a proper reaction buffer in vitro. Polymerase chain reaction (PCR) is a valuable tool for monitoring gene expression, for quantifying foodborne pathogens, and for use in clinical diagnostics. Hybridization methods offer a new frontier in the identification of targets in a simple and high-throughput way. This review mainly describes and compares the basic rules of PCR and hybridization, including their formatting source, characteristics, and applications.


Principle Base pairing Recognition PCR HCR 



This work is supported by the Ministry of Science and Technology of Beijing (XX2014B069). Many thanks to Chenguang Wang, for his kind help in manuscript conception and preparation.


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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • Wentao Xu
    • 1
    • 2
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina

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