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Adaptable Methods to Extract Nucleic Acid Targets and Evaluate Quality

  • Wentao Xu
Chapter

Abstract

Extraction of nucleic acid targets (DNA and RNA) is a basic method that is used in molecular biology. In recent years, nucleic acid analysis has become an important analytical tool in many fields, demanding high-quality DNA or RNA. Nucleic acid targets can be extracted from various materials for subsequent processes, for example, PCR test. To date, many different ways have been developed to extract nucleic acid, such as column-based and solution-based methods. For the success of subsequent tests, extracting protocols should result in high purity, yield, and reproducibility of the nucleic acid targets, and the accuracy, speed, and reliability of the protocols should be maximal, while the contamination should be minimized.

Keywords

Nucleic acid extraction Sample preparation Cell lysis Purification DNA quantification 

Notes

Acknowledgments

This work is supported by the National Science and Technology Major Project (2016ZX08012-004). Many thanks to Wenying Tian and Xingtian Cui for their kind help in manuscript conception and preparation.

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© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • Wentao Xu
    • 1
    • 2
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina

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