The Identification and Detection Technology of Research in Microorganisms Including Living or Dead Bacteria

  • Wentao Xu


Both dead and living bacteria can affect food quality. In recent years, the development of microbiological testing has undergone a series of progressions from traditional methods—including microbial cultivation, physiological and biochemical testing, instrument analysis, and immunology—to molecular biological detection. Reviewing its developments, it can be seen that the detection is becoming more rapid, sensitive, precise, integrated, and low cost. Of course, when all methods of microbial detection are compared, only living bacteria that detected by traditional methods could yield false negative results, especially if there are dormant bacteria. But with molecular methods, all microorganisms can be detected. With the development of technology, a lot of focus has been given to identification and detection technology for dead and living bacteria, which has massive potential and important significance in the area of microorganisms. There exists prospects of novel detection techniques for discrimination of viable and nonviable microorganisms, especially some more sensitive, precise, and comprehensive ones.


Viable Nonviable Bacteria Nucleic acid analysis Amplification 



This work is supported by the Ministry of Science and Technology of Beijing (XX2014B069). Many thanks to Yuancong Xu, for her kindly help in manuscript conception and preparation.


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Copyright information

© Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • Wentao Xu
    • 1
    • 2
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional EngineeringChina Agricultural UniversityBeijingChina

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