Abstract
Food adulteration is a major concern due to the increased awareness among consumers with regard to the safety of foods and the need to verify labeling statements. In this study, we have developed a PCR assay for detecting porcine DNA in raw meat and meat-based processed food products. The PCR assay also included an in-house, simple DNA extraction method. Positive results were obtained from genomic DNA extracted from raw meats and meat-based food products tested. The sensitivity of the assay was found to be 1 pg and 0.5 % adulterated (pork + bovine) meat. The assay was proven to be effective in detecting pork meat in raw and processed food.
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Ahmed, S.A., Pin, L.L., Raabe, C.A., Rozhdestvensky, T.S., Hock, T.T. (2016). A Combined Rapid DNA Extraction and Multiplex PCR for the Detection of Porcine DNA in Raw and Processed Food. In: Ab. Manan, S., Abd Rahman, F., Sahri, M. (eds) Contemporary Issues and Development in the Global Halal Industry. Springer, Singapore. https://doi.org/10.1007/978-981-10-1452-9_18
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DOI: https://doi.org/10.1007/978-981-10-1452-9_18
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