Abstract
In this chapter, synthesized copolymer compositions are processed in order to fabricate coated biochips by using spin-coating technique. Developed biochips have subsequently been used in sandwich ELISA experiment via three different techniques: physical immobilization; covalent immobilization; and immobilization through amine-bearing spacers. In this chapter, principles behind each of the mentioned methods are described in a great detail. The major emphasis of the chapter is on the characterization of the developed biochips. In that path, morphology and topography of the coated platforms have thoroughly been investigated. Moreover, wettability of the polymer coated chips before and after treatment with different techniques were also investigated. Proposed platforms from both categories of treated and untreated, then, were analyzed by XPS analysis to study surface chemistry of the fabricated bioreceptors. This chapter has also discussed the correlation of obtained data from each characterization technique to another in a very comprehensive manner.
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Hosseini, S., Ibrahim, F. (2016). Biochips Fabrication and Surface Characterization. In: Novel Polymeric Biochips for Enhanced Detection of Infectious Diseases. SpringerBriefs in Applied Sciences and Technology(). Springer, Singapore. https://doi.org/10.1007/978-981-10-0107-9_3
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DOI: https://doi.org/10.1007/978-981-10-0107-9_3
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