Soft X-ray Radiation Applied in the Analysis of Intact Viruses and Antibodies by Means of Nano Electrospray Differential Mobility Analysis
The analysis of an intact infectious virus and a therapeutic monoclonal antibody with a nano electrospray differential mobility analyzer, a kind of ion mobility device, (nES-DMA aka nES GEMMA, macroIMS or SMPS) incorporating a commercial soft X-ray (SXR) source for charge reduction was successfully demonstrated representing a clear alternative to a 210Po-based charge reducing source for bio nanoparticles. Not only bio nanoparticle detection and size determination will be shown with the SXR device but also particle number-based quantification demonstrated with an antibody. These achievements will open up new avenues in life sciences in general as well as in biomonitoring.
The authors acknowledge funding by the Austrian Science Foundation (FWF) TRP29. Furthermore we thank D. Blaas (Vienna, Austria) for supplying the virus samples and F. Foret (Brno, Czech Republic) for the help of designing the spray capillary tip grinding device.
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